FigsharePub Date : 2024-06-26DOI: 10.1021/acssynbio.4c00178.s001
Sara M. Eslami (18889074), Chandrashekhar Padhi (18347453), Imran R. Rahman (14685930), Wilfred A. van der Donk (7471073)
{"title":"Expression andnSubcellular Localization of Lanthipeptidesnin Human Cells","authors":"Sara M. Eslami (18889074), Chandrashekhar Padhi (18347453), Imran R. Rahman (14685930), Wilfred A. van der Donk (7471073)","doi":"10.1021/acssynbio.4c00178.s001","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00178.s001","url":null,"abstract":"Cyclic peptides,nsuch as most ribosomally synthesized and post-translationallynmodified peptides (RiPPs), represent a burgeoning area of interestnin therapeutic and biotechnological research because of their conformationalnconstraints and reduced susceptibility to proteolytic degradationncompared to their linear counterparts. Herein, an expression systemnis reported that enables the production of structurally diverse lanthipeptidesnand derivatives in mammalian cells. Successful targeting of lanthipeptidesnto the nucleus, the endoplasmic reticulum, and the plasma membranenis demonstrated. In vivo expression and targeting of such peptidesnin mammalian cells may allow for screening of lanthipeptide-basedncyclic peptide inhibitors of native, organelle-specific protein–proteinninteractions in mammalian systems.","PeriodicalId":523403,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}