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Leoligin, the Major Lignan from Edelweiss (Leontopodium nivale subsp. alpinum), Promotes Cholesterol Efflux from THP‑1 Macrophages Leoligin，来自雪绒花的主要木脂素（Leontopodium nivale subsp.）alpinum)，促进THP‑1巨噬细胞的胆固醇外排

Figshare Pub Date : 2016-06-20 DOI: 10.1021/acs.jnatprod.6b00227.s001

Limei Wang (77943), Angela Ladurner (604954), Simone Latkolik (2803192), Stefan Schwaiger (388587), Thomas Linder (2259202), Jan Hošek (1569946), Veronika Palme (2803195), Nicole Schilcher (2803201), Ondřej Polanský (2803198), Elke H. Heiss (404988), Herbert Stangl (595982), Marko D. Mihovilovic (343636), Hermann Stuppner (388594), Verena M. Dirsch (404989), Atanas G. Atanasov (27419)

{"title":"Leoligin, the Major Lignan from Edelweiss (Leontopodium nivale subsp. alpinum), Promotes Cholesterol Efflux from THP‑1 Macrophages","authors":"Limei Wang (77943), Angela Ladurner (604954), Simone Latkolik (2803192), Stefan Schwaiger (388587), Thomas Linder (2259202), Jan Hošek (1569946), Veronika Palme (2803195), Nicole Schilcher (2803201), Ondřej Polanský (2803198), Elke H. Heiss (404988), Herbert Stangl (595982), Marko D. Mihovilovic (343636), Hermann Stuppner (388594), Verena M. Dirsch (404989), Atanas G. Atanasov (27419)","doi":"10.1021/acs.jnatprod.6b00227.s001","DOIUrl":"https://doi.org/10.1021/acs.jnatprod.6b00227.s001","url":null,"abstract":"Leoligin is a natural lignan foundnin Edelweiss (Leontopodium nivale ssp. alpinum). The aim of this study was to examine its influence on cholesterolnefflux and to address the underlying mechanism of action. Leoliginnincreases apo A1- as well as 1% human plasma-mediated cholesterolnefflux in THP-1 macrophages without affecting cell viability as determinednby resazurin conversion. Western blot analysis revealed that the proteinnlevels of the cholesterol efflux transporters ABCA1 and ABCG1 werenupregulated, whereas the SR-B1 protein level remained unchanged uponntreatment with leoligin (10 μM, 24 h). Quantitative reversentranscription PCR further uncovered that leoligin also increased ABCA1nand ABCG1 mRNA levels without affecting the half-life of the two mRNAsnin the presence of actinomycin D, a transcription inhibitor. Proteomenanalysis revealed the modulation of protein expression fingerprintnin the presence of leoligin. Taken together, these results suggestnthat leoligin induces cholesterol efflux in THP-1-derived macrophagesnby upregulating ABCA1 and ABCG1 expression. This novel activity suggestsnleoligin as a promising candidate for further studies addressing anpossible preventive or therapeutic application in the context of atherosclerosis.","PeriodicalId":523357,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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