FigsharePub Date : 1753-01-01DOI: 10.1021/acs.jafc.4c04882.s001
Kuaitian Wang (16428978), Mengqi Jian (17906567), Yuhang Chen (584479), Mengdi Du (16428981), Zhenhua Wang (426041), Bo Xu (233514), Ning Tang (138218), Yongqiang Cheng (1428478), Jing Gan (7154414)
{"title":"Soy Peptide AmelioratenTGF-β1-Mediated OsteoblastnDifferentiation through Smad and MAPK Signaling Pathways","authors":"Kuaitian Wang (16428978), Mengqi Jian (17906567), Yuhang Chen (584479), Mengdi Du (16428981), Zhenhua Wang (426041), Bo Xu (233514), Ning Tang (138218), Yongqiang Cheng (1428478), Jing Gan (7154414)","doi":"10.1021/acs.jafc.4c04882.s001","DOIUrl":"https://doi.org/10.1021/acs.jafc.4c04882.s001","url":null,"abstract":"The aim of this study was to determine the osteogenicnactivitynand mechanism of soybean peptide VVELLKAFEEKF (SOP) and the potentialnrelationship between SOP and transforming growth factor-β1 (TGF-β1).nThe results show that SOP promotes MC3T3-E1 cell proliferation bynaltering cell progression. SOP induced cell differentiation and mineralizationnin a dose-dependent manner at 0.7–7 μM. Moreover, SOPnstimulates osteoblast differentiation, which may be achieved throughnthe activation of p38-MAPK and Smad2/3 signaling pathways. Furthermore,ntreatment with a TβRI inhibitor (SB525334) inhibited the phosphorylationnlevels of p38 and Smad2/3, which indicates the involvement of TβRInin the process of osteoblast differentiation caused by SOP. Besides,nin non-FBS-cultured MC3T3-E1 cells, SOP and TGF-β1 promotednthe phosphorylation of Smad2/3 and alkaline phosphatase (ALP) activity,nbut the effect was lost when SOP was incubated separately, indicatingnthat SOP stimulated osteoblast differentiation by promoting TGF-β1nactivity. <i>In vivo</i>, SOP significantly restores bonenmineral density loss and behavioral deficits in a model of glucocorticoid-inducednosteoporosis (GIOP) in zebrafish. These results suggest that SOP maynhave the function of promoting bone remodeling and may be used asna potential active factor for functional food development to preventnosteoporosis.","PeriodicalId":523217,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1753-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://figshare.com/articles/journal_contribution/Soy_Peptide_Ameliorate_TGF-_1-Mediated_Osteoblast_Differentiation_through_Smad_and_MAPK_Signaling_Pathways/27210576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147911817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}