{"title":"Synergynof Nanotopography and Electrical Conductivitynof PEDOT/PSS for Enhanced Neuronal Development","authors":"Michele Bianchi (2129686), Sonia Guzzo (7379435), Alice Lunghi (17628512), Pierpaolo Greco (2407132), Alessandra Pisciotta (117491), Mauro Murgia (1567390), Gianluca Carnevale (117495), Luciano Fadiga (41646), Fabio Biscarini (1302546)","doi":"10.1021/acsami.3c15278.s003","DOIUrl":"https://doi.org/10.1021/acsami.3c15278.s003","url":null,"abstract":"Biomaterials ablento promote neuronal development and neurite outgrowthnare highly desired in neural tissue engineering for the repair ofndamaged or disrupted neural tissue and restoring the axonal connection.nFor this purpose, the use of either electroactive or micro- and nanostructurednmaterials has been separately investigated. Here, the use of a nanomodulatednconductive poly(3,4-ethylendioxithiophene) poly(styrenesulfonate)n(PEDOT/PSS) substrate that exhibits instructive topographical andnelectrical cues at the same time was investigated for the first time.nIn particular, thin films featuring grooves with sizes comparablenwith those of neuronal neurites (NanoPEDOT) were fabricated by electrochemicalnpolymerization of PEDOT/PSS on a nanomodulated polycarbonate template.nThe ability of NanoPEDOT to support neuronal development and directnneurite outgrowth was demonstrated by assessing cell viability andnproliferation, expression of neuronal markers, average neurite length,nand direction of neuroblastoma N2A cells induced to differentiatenon this novel support. In addition to the beneficial effect of thennanogrooved topography, a 30% increase was shown in the average lengthnof neurites when differentiating cells were subjected to an electricalnstimulation of a few microamperes for 6 h. The results reported herensuggest a favorable effect on the neuronal development of the synergisticncombination of nanotopography and electrical stimulation, supportingnthe use of NanoPEDOT in neural tissue engineering to promote physicalnand functional reconnection of impaired neural networks.","PeriodicalId":522982,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}