FigsharePub Date : 2019-04-17DOI: 10.1021/acsbiomaterials.9b00423.s001
Lidya Abune (4218034), Nan Zhao (351674), Jinping Lai (1596097), Benjamin Peterson (5895257), Spencer Szczesny (6632087), Yong Wang (12837)
{"title":"Macroporous Hydrogels for Stable Sequestration andnSustained Release of Vascular Endothelial Growth Factor and BasicnFibroblast Growth Factor Using Nucleic Acid Aptamers","authors":"Lidya Abune (4218034), Nan Zhao (351674), Jinping Lai (1596097), Benjamin Peterson (5895257), Spencer Szczesny (6632087), Yong Wang (12837)","doi":"10.1021/acsbiomaterials.9b00423.s001","DOIUrl":"https://doi.org/10.1021/acsbiomaterials.9b00423.s001","url":null,"abstract":"Macroporousnhydrogels have been widely studied for biological andnbiomedical applications such as drug delivery and tissue engineering.nHowever, these hydrogels cannot stably sequester molecules of interestndue to their high permeability. The purpose of this work was to studynthe feasibility of using two aptamers to sequester two protein drugs,nquantify the apparent diffusivity of the protein drugs in aptamer-functionalizednmacroporous hydrogels, and evaluate the function of aptamer-functionalizednmacroporous hydrogels in controlling protein release for angiogenesis.nVascular endothelial growth factor (VEGF) and basic fibroblast growthnfactor (bFGF) were used as the model proteins. The data show thatnanti-VEGF and anti-bFGF aptamers could be uniformly incorporated intonmacroporous hydrogels for stable and specific sequestration of VEGFnand bFGF. The aptamers could reduce the apparent diffusivity of VEGFnand bFGF in the macroporous hydrogels by approximately 3 orders ofnmagnitude. Moreover, as the aptamers could prolong the release ofnthese growth factors, dual aptamer-functionalized macroporous hydrogelsncould stimulate synergistic angiogenesis. Therefore, this work hasnsuccessfully demonstrated that aptamer-functionalized macroporousnhydrogels hold great potential of stably sequestering multiple moleculesnof interest for various biological and biomedical applications.","PeriodicalId":522893,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}