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Genome-Wide DNA Methylome and Transcriptome Analysisnof Porcine Intestinal Epithelial Cells upon Deoxynivalenol Exposure 脱氧雪腐镰刀菌醇暴露后猪肠上皮细胞全基因组DNA甲基组和转录组分析

Figshare Pub Date : 2019-04-23 DOI: 10.1021/acs.jafc.9b00613.s001

Haifei Wang (435950), Qiufang Zong (6724151), Shiqin Wang (4049413), Chengxiang Zhao (6724154), Shenglong Wu (140544), Wenbin Bao (140546)

{"title":"Genome-Wide DNA Methylome and Transcriptome Analysisnof Porcine Intestinal Epithelial Cells upon Deoxynivalenol Exposure","authors":"Haifei Wang (435950), Qiufang Zong (6724151), Shiqin Wang (4049413), Chengxiang Zhao (6724154), Shenglong Wu (140544), Wenbin Bao (140546)","doi":"10.1021/acs.jafc.9b00613.s001","DOIUrl":"https://doi.org/10.1021/acs.jafc.9b00613.s001","url":null,"abstract":"Deoxynivalenoln(DON) is a type of mycotoxin that is disruptivento intestinal and immune systems. To better understand the molecularneffects of DON exposure, we performed genome-wide comparisons of DNAnmethylation and gene expression from porcine intestinal epithelialncell IPEC-J2 upon DON exposure using reduced representation bisulfitensequencing and RNA-seq technologies. We characterized the methylationnpattern changes and found 3030 differentially methylated regions.nMoreover, 3226 genes showing differential expression were enrichednin pathways of protein and nucleic acid synthesis and ribosome biogenesis.nIntegrative analysis identified 29 genes showing inverse correlationsnbetween promoter methylation and expression. Altered DNA methylationnand expression of various genes suggested their roles and potentialnfunctional interactions upon DON exposure. Our data provided new insightsninto epigenetic and transcriptomic alterations of intestinal epithelialncells upon DON exposure and may advance the identification of biomarkersnand drug targets for predicting and controlling the toxic effectsnof this common mycotoxin.","PeriodicalId":522871,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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