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Linoleate Isomerase Complex Contributes to Metabolismnand Remission of DSS-Induced Colitis in Mice of Lactobacillusnplantarum ZS2058 亚油酸异构酶复合物在植物乳杆菌ZS2058诱导的小鼠结肠炎的代谢和缓解中的作用

Figshare Pub Date : 2021-07-20 DOI: 10.1021/acs.jafc.1c02944.s001

Bo Yang (104813), He Gao (86117), Hui Qi (460415), Yang Chen (9737), R. Paul Ross (152753), Catherine Stanton (223943), Jianxin Zhao (640885), Hao Zhang (15339), Haiqin Chen (193169), Wei Chen (23863)

{"title":"Linoleate Isomerase Complex Contributes to Metabolismnand Remission of DSS-Induced Colitis in Mice of Lactobacillusnplantarum ZS2058","authors":"Bo Yang (104813), He Gao (86117), Hui Qi (460415), Yang Chen (9737), R. Paul Ross (152753), Catherine Stanton (223943), Jianxin Zhao (640885), Hao Zhang (15339), Haiqin Chen (193169), Wei Chen (23863)","doi":"10.1021/acs.jafc.1c02944.s001","DOIUrl":"https://doi.org/10.1021/acs.jafc.1c02944.s001","url":null,"abstract":"A linoleate isomerase complex includingnmyosin-cross-reactive antigen,nshort-chain dehydrogenase/oxidoreductase, and acetoacetate decarboxylasenhas been confirmed as the pivotal factor for conjugated linoleic acidn(CLA) production in Lactobacillus plantarum. However, its role in the metabolism and health-associated benefitsnof Lactobacillus remain unclear. Innthe current study, the mild type, knockout, and complemented mutantsnof the linoleate isomerase complex of L. plantarum ZS2058 were used to investigate those putative effects. The metabonomicnresults showed that a linoleate isomerase complex could significantlyninfluence the glycol-metabolism, lipid metabolism, and antioxidantncompounds. Especially, with the stress of linoleic acid, linoleatenisomerase complex knockout mutants induced the increase of severalnantioxidant compounds, such as glutamic acid, glycine, l-cysteine,nglycerol, and l-sorbosone. Moreover, the linoleate isomerasencomplex played a pivotal role in ameliorating DSS-induced colitis.nThe knockout mutants showed effects similar to those in the DSS group,nwhereas complementation of the corresponding gene in the knockoutnmutants could restore the anti-inflammatory activity, wherein thenintegrity of a mucus layer was repaired, the level of pro-inflammatoryncytokines decreased, and the amount of anti-inflammatory cytokinesnincreased significantly. All the results indicated that the linoleatenisomerase complex plays a key role in CLA production and metabolismnas well as the health-associated benefits of L. plantarum ZS2058. These results are conducive to promote clinical trials andnproduct development of probiotics for colitis.","PeriodicalId":522776,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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