FigsharePub Date : 2018-02-16DOI: 10.1021/acs.analchem.7b03587.s001
Yunlong Chen (1814557), Huipu Liu (4859941), Lin Ding (211686), Huangxian Ju (392237)
{"title":"Functional Dual-Color Indicator To Achieve in SitunVisualization of Intracellular Glycosylation","authors":"Yunlong Chen (1814557), Huipu Liu (4859941), Lin Ding (211686), Huangxian Ju (392237)","doi":"10.1021/acs.analchem.7b03587.s001","DOIUrl":"https://doi.org/10.1021/acs.analchem.7b03587.s001","url":null,"abstract":"A functional dual-color indicatornis designed for in situ visualizationnof intracellular glycosylation. Using O-GlcNAcylation as model, thenindicator is constructed on a poly-GlcNAc-coated gold nanoparticlen(AuNP) by assembling dye labeled lectin (FSWGA) and then another dye-labelednGlcNAc (FGlcNAc) through the two opposite subunits of FSWGA. Thesendyes possess negligible overlapping emission and can be quenched bynAuNP. In the presence of intracellular dissociated GlcNAc residuenand O-GlcNAcylated proteins, the assembled FGlcNAc and the conjugatenof FSWGA with FGlcNAc are released from AuNP through the dynamic competitivenconjugation, which lights up the fluorescence of two dyes, respectively,nand provides a simple technique for simultaneously monitoring thenlevel of O-GlcNAcylated proteins and the total amount of GlcNAc groupsnin living cells. The practicality of the protocol for visually monitoringnthe biological pathway between intracellular O-GlcNAcylation and cellnsurface differentiation-related proteins demonstrates a convenientnand powerful tool for research of glycosylation equilibrium and relatednbiological processes.","PeriodicalId":522765,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}