FigsharePub Date : 2021-08-09DOI: 10.1021/acschemneuro.1c00059.s001
Bei Sun (309806), Hao Ou (6005645), Fei Ren (172060), Yujiao Guan (9363861), Ye Huan (6005648), Hongwei Cai (495979)
{"title":"Propofol Protects against Cerebral Ischemia/ReperfusionnInjury by Down-Regulating Long Noncoding RNA SNHG14","authors":"Bei Sun (309806), Hao Ou (6005645), Fei Ren (172060), Yujiao Guan (9363861), Ye Huan (6005648), Hongwei Cai (495979)","doi":"10.1021/acschemneuro.1c00059.s001","DOIUrl":"https://doi.org/10.1021/acschemneuro.1c00059.s001","url":null,"abstract":"Cerebralnischemia-reperfusion (CI/R) injury is a serious centralnnervous system disease. Propofol (PPF) exerts a neuroprotective effectnin CI/R injury; the underlying cause is still unclear. Here, we culturednmouse hippocampal neuron (HT22 cells) in oxygen-glucose deprivation/reoxygenationn(OGD/R) conditions to mimic CI/R injury <i>in vitro</i>.nPPF treatment promoted cell viability and reduced apoptotic cellsnin the OGD/R-treated HT22 cells, which was effectively abrogated bynSNHG14 overexpression. Moreover, we constructed a CI/R injury mousenmodel on C57BL/6J mice by middle cerebral artery occlusion/reperfusionn(MCAO/R), followed by administration of PPF. PPF reduced neuronalndamage and loss, enhanced glial cell hyperplasia, and amelioratedncerebral cortex tissue damage and brain infarct in MCAO/R-inducednmice. SNHG14 overexpression aggravated MCAO/R-induced CI/R injurynin mice. Furthermore, SNHG14 promoted the expression of Atg5 and Beclinn1 via competitively binding miR-30b-5p, which contributed to activatenautophagy and apoptosis in HT22 cells. In addition, the levels ofnp-p38 and p-SP1 were reduced in the OGD/R-treated HT22 cells in thenpresence of PPF. SP1 interacted with the promoter of SNHG14 and elevatednthe expression of SNHG14. PPF treatment inhibited the SP1-mediatednup-regulation of SNHG14. In conclusion, this work demonstrates thatnPPF inhibits SNHG14 expression though the p38 MAPK signaling pathway.nSNHG14 promotes Atg5 and Beclin 1 expression by sponging miR-30b-5pnand thus activates autophagy and aggravates CI/R injury.","PeriodicalId":522555,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://figshare.com/articles/journal_contribution/Propofol_Protects_against_Cerebral_Ischemia_Reperfusion_Injury_by_Down-Regulating_Long_Noncoding_RNA_SNHG14/15134870","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}