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Nuciferine AmelioratesnNonesterified Fatty Acid-InducednBovine Mammary Epithelial Cell Lipid Accumulation, Apoptosis, andnImpaired Migration via Activating LKB1/AMPK Signaling Pathway 荷叶碱通过激活LKB1/AMPK信号通路改善非酯化脂肪酸诱导的牛乳腺上皮细胞脂质积累、凋亡和迁移受损

Figshare Pub Date : 1753-01-01 DOI: 10.1021/acs.jafc.2c06133.s001

Jinxia Li (1873816), Chenchen Zhao (7624115), Menglin Liu (2848241), Linfang Chen (13244629), Yiwei Zhu (1419013), Wenwen Gao (2792551), Xiliang Du (11531908), Yuxiang Song (5181290), Xinwei Li (430518), Guowen Liu (1635484), Lin Lei (400470), Haihua Feng (198813)

{"title":"Nuciferine AmelioratesnNonesterified Fatty Acid-InducednBovine Mammary Epithelial Cell Lipid Accumulation, Apoptosis, andnImpaired Migration via Activating LKB1/AMPK Signaling Pathway","authors":"Jinxia Li (1873816), Chenchen Zhao (7624115), Menglin Liu (2848241), Linfang Chen (13244629), Yiwei Zhu (1419013), Wenwen Gao (2792551), Xiliang Du (11531908), Yuxiang Song (5181290), Xinwei Li (430518), Guowen Liu (1635484), Lin Lei (400470), Haihua Feng (198813)","doi":"10.1021/acs.jafc.2c06133.s001","DOIUrl":"https://doi.org/10.1021/acs.jafc.2c06133.s001","url":null,"abstract":"High blood concentrations of nonesterified fatty acidsn(NEFAs)nprovoke various metabolic disorders and are associated with mammaryntissue injury and decreased milk production in dairy cows. Nuciferine,nan alkaloid found in Nelumbo nucifera leaves, has great potential for correcting lipid metabolism derangementsnand lipotoxicity. In this study, we evaluated the lipotoxicity inducednby excessive NEFA in bovine mammary epithelial cells (bMECs) and investigatednwhether nuciferine alleviates NEFA-induced lipotoxicity and the underlyingnmolecular mechanisms. We found that excessive NEFA (1.2 and 2.4 mM)ninduced lipid accumulation, apoptosis, and migration ability impairmentnin bMECs, whereas nuciferine could ameliorate these disarrangements,nas indicated by decreasing triglyceride content, protein abundancenof SREBP-1c, cytoplasmic cytochrome <i>c</i>, and cleavedncaspase-3 and increasing protein abundance of PPARα and migrationnability. Moreover, nuciferine could reverse NEFA-induced LKB1/AMPKnsignaling inhibition, and the protective effect of nuciferine on lipotoxicityncaused by NEFA was abrogated by AMPK inhibitor dorsomorphin. Furthermore,ntransfection with LKB1 siRNA (si-LKB1) largely abolished the activationneffect of nuciferine on AMPK. Overall, nuciferine can protect bMECsnfrom excessive NEFA-induced lipid accumulation, apoptosis, and impairednmigration by activating LKB1/AMPK signaling pathway.","PeriodicalId":522525,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1753-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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