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Mycosome-Coated GoldnNanoparticles for Plasmonic Detectionnof Tuberculosis-Associated Antibodies 用于结核相关抗体等离子体检测的金纳米粒包被真菌体

Figshare Pub Date : 2024-03-15 DOI: 10.1021/acsanm.4c00706.s001

ArmandoH. Becerra (18166645), Mariana G. Flores (18166648), José P. Palma-Nicolas (18166651), Gonzalo Ramírez-García (6243362), Luz M. López-Marín (18166654)

{"title":"Mycosome-Coated GoldnNanoparticles for Plasmonic Detectionnof Tuberculosis-Associated Antibodies","authors":"ArmandoH. Becerra (18166645), Mariana G. Flores (18166648), José P. Palma-Nicolas (18166651), Gonzalo Ramírez-García (6243362), Luz M. López-Marín (18166654)","doi":"10.1021/acsanm.4c00706.s001","DOIUrl":"https://doi.org/10.1021/acsanm.4c00706.s001","url":null,"abstract":"Considering that more than 10 million people developntuberculosisndisease (TB) each year and that nearly 3 million of them are neverndiagnosed, label-free screening methods constitute one of the topnpublic health priorities worldwide. Herein we report the fabricationnof a plasmonic system for the detection of TB-associated antibodies.nGold nanoparticles (AuNPs) were synthesized at controlled sizes andnmodified to display antigen lipid glycans from mycobacteria, the groupnof organisms comprising the TB causal agent. Facile self-assemblynprocedures were employed to form mycobacterial phosphatidylinositolnmannoside (PIM)-containing unilamellar liposomes (mycosomes) and tonattach them onto gold surfaces using thiol chemistry. Using enzyme-linkednimmunoassays with rabbit hyperimmune sera, the constructs were foundnto specifically capture antilipid antibodies, presenting a dose-dependentnresponse. Also, the functional hybrid nanoparticles showed localizednsurface plasmon resonance (LSPR) shifts of up to 2 nm upon their interactionnwith specific antibodies, indicating their suitability for the opticalntransduction of antigen–antibody reactions. Finally, the applicabilitynof the system for real-world detection of TB-associated antibodiesnwas demonstrated through analysis of 12 samples of human individuals,nincluding 7 cases with pulmonary TB. To the best of our knowledge,nthis is the first biomimetic nanogold–lipid system for thenimplementation of lipids as immunosensing reagents for the plasmonicndetection and diagnosis of TB.","PeriodicalId":522229,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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