FigsharePub Date : 2019-01-22DOI: 10.1021/acsnano.8b09462.s001
Duc H.T. Le (1972795), Ulrich Commandeur (2326699), Nicole F. Steinmetz (1311336)
{"title":"Presentation and Delivery of Tumor Necrosis Factor-RelatednApoptosis-Inducing Ligand <i>via</i> Elongated Plant ViralnNanoparticle Enhances Antitumor Efficacy","authors":"Duc H.T. Le (1972795), Ulrich Commandeur (2326699), Nicole F. Steinmetz (1311336)","doi":"10.1021/acsnano.8b09462.s001","DOIUrl":"https://doi.org/10.1021/acsnano.8b09462.s001","url":null,"abstract":"Potatonvirus X (PVX) is a flexuous plant virus-based nanotechnologynwith promise in cancer therapy. As a high aspect ratio biologic (13n× 515 nm), PVX has excellent spatial control in structures andnfunctions, offering high-precision nanoengineering for multivalentndisplay of functional moieties. Herein, we demonstrate the preparationnof the PVX-based nanocarrier for delivery of tumor necrosis factor-relatednapoptosis-inducing ligand (TRAIL), a promising protein drug that inducesnapoptosis in cancer cells but not healthy cells. TRAIL bound to PVXnby coordination bonds between nickel-coordinated nitrilotriaceticnacid on PVX and His-tag on the protein could mimic the bioactive “membrane-bound”nstate in native TRAIL, resulting in an elongated nanoparticle displayingnup 490 therapeutic protein molecules. Our data show that PVX-deliverednTRAIL activates caspase-mediated apoptosis more efficiently comparednto soluble TRAIL; also <i>in vivo</i> the therapeutic nanoparticlenoutperforms in delaying tumor growth in an athymic nude mouse modelnbearing human triple-negative breast cancer xenografts. This proof-of-conceptnwork highlights the potential of filamentous plant virus nanotechnologies,nparticularly for targeting protein drug delivery for cancer therapy.","PeriodicalId":522227,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}