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TowardnBrain-on-a-Chip: Human Induced PluripotentnStem Cell-Derived Guided Neuronal Networks in Tailor-Made 3D NanoprintednMicroscaffolds 迈向芯片上的大脑：人类诱导的多能干细胞衍生的神经网络在定制的纳米3D打印纳米微支架中

Figshare Pub Date : 2020-10-15 DOI: 10.1021/acsnano.0c04640.s003

Jann Harberts (4762368), Cornelius Fendler (9526049), Jeremy Teuber (9526052), Malte Siegmund (9526055), Aaron Silva (9526058), Niklas Rieck (9526061), Merle Wolpert (9526064), Robert Zierold (1612243), Robert H. Blick (1949341)

{"title":"TowardnBrain-on-a-Chip: Human Induced PluripotentnStem Cell-Derived Guided Neuronal Networks in Tailor-Made 3D NanoprintednMicroscaffolds","authors":"Jann Harberts (4762368), Cornelius Fendler (9526049), Jeremy Teuber (9526052), Malte Siegmund (9526055), Aaron Silva (9526058), Niklas Rieck (9526061), Merle Wolpert (9526064), Robert Zierold (1612243), Robert H. Blick (1949341)","doi":"10.1021/acsnano.0c04640.s003","DOIUrl":"https://doi.org/10.1021/acsnano.0c04640.s003","url":null,"abstract":"Brain-on-a-chipn(BoC) concepts should consider three-dimensionaln(3D) scaffolds to mimic the 3D nature of the human brain not accessiblenby conventional planar cell culturing. Furthermore, the essentialnkey to adequately address drug development for human pathophysiologicalndiseases of the nervous system, such as Parkinson’s or Alzheimer’s,nis to employ human induced pluripotent stem cell (iPSC)-derived neuronsninstead of neurons from animal models. To address both issues, wenpresent electrophysiologically mature human iPSC-derived neurons culturednin BoC applicable microscaffolds prepared by direct laser writing.n3D nanoprinted tailor-made elevated cavities interconnected by freestandingnmicrochannels were used to create defined neuronal networksasna proof of conceptwith two-dimensional topology. The neuronalnoutgrowth in these nonplanar structures was investigated, among others,nin terms of neurite length, size of continuous networks, and branchingnbehavior using <i>z</i>-stacks prepared by confocal microscopynand cross-sectional scanning electron microscopy images prepared bynfocused ion beam milling. Functionality of the human iPSC-derivednneurons was demonstrated with patch clamp measurements in both current-nand voltage-clamp mode. Action potentials and spontaneous excitatorynpostsynaptic currentsfundamental prerequisites for propernnetwork signalingprove full integrity of these artificialnneuronal networks. Considering the network formation occurring withinnonly a few days and the versatile nature of direct laser writing toncreate even more complex scaffolds for 3D network topologies, we believenthat our study offers additional approaches in human disease researchnto mimic the complex interconnectivity of the human brain in BoC studies.","PeriodicalId":522195,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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