Figshare最新文献

筛选

英文中文

Tuning the Color Palette of Fluorescent Copper Sensorsnthrough Systematic Heteroatom Substitution at Rhodol Cores 通过Rhodol核的系统杂原子取代调整荧光铜传感器的调色板

Figshare Pub Date : 2017-11-07 DOI: 10.1021/acschembio.7b00748.s001

Shang Jia (1958947), Karla M. Ramos-Torres (4576273), Safacan Kolemen (1789222), Cheri M. Ackerman (4576276), Christopher J. Chang (151940)

{"title":"Tuning the Color Palette of Fluorescent Copper Sensorsnthrough Systematic Heteroatom Substitution at Rhodol Cores","authors":"Shang Jia (1958947), Karla M. Ramos-Torres (4576273), Safacan Kolemen (1789222), Cheri M. Ackerman (4576276), Christopher J. Chang (151940)","doi":"10.1021/acschembio.7b00748.s001","DOIUrl":"https://doi.org/10.1021/acschembio.7b00748.s001","url":null,"abstract":"Coppernis an essential nutrient for sustaining life, and emergingndata have expanded the roles of this metal in biology from its canonicalnfunctions as a static enzyme cofactor to dynamic functions as a transitionnmetal signal. At the same time, loosely bound, labile copper poolsncan trigger oxidative stress and damaging events that are detrimentalnif misregulated. The signal/stress dichotomy of copper motivates thendevelopment of new chemical tools to study its spatial and temporalndistributions in native biological contexts such as living cells.nHere, we report a family of fluorescent copper sensors built uponncarbon-, silicon-, and phosphorus-substituted rhodol dyes that enablensystematic tuning of excitation/emission colors from orange to near-infrared.nThese probes can detect changes in labile copper levels in livingncells upon copper supplementation and/or depletion. We demonstratenthe ability of the carbon–rhodol based congener, Copper CarbonFluor 1 (CCF1), to identify elevations in labile copper pools in thenAtp7a<sup>–/–</sup> fibroblast cell model of the geneticncopper disorder Menkes disease. Moreover, we showcase the utilitynof the red-emitting phosphorus-rhodol based dye Copper PhosphorusnFluor 1 (CPF1) in dual-color, dual-analyte imaging experiments withnthe green-emitting calcium indicator Calcium Green-1 to enable simultaneousndetection of fluctuations in copper and calcium pools in living cells.nThe results provide a starting point for advancing tools to studynthe contributions of copper to health and disease and for exploitingnthe rapidly growing palette of heteroatom-substituted xanthene dyesnto rationally tune the optical properties of fluorescent indicatorsnfor other biologically important analytes.","PeriodicalId":522160,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0