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Ultrafast Screening of a Novel, Moderately Hydrophilic Angiotensin-Converting-Enzyme-InhibitorynPeptide, RYL, from Silkworm Pupa Using an Fe-Doped-Silkworm-Excrement-DerivednBiocarbon: Waste Conversion by Waste 利用铁掺杂的蚕粪衍生生物炭从蚕蛹中超快速筛选一种新型的中等亲水性血管紧张素转化酶抑制肽RYL

Figshare Pub Date : 2017-12-15 DOI: 10.1021/acs.jafc.7b04442.s001

Long Liu (390523), Yanan Wei (3768307), Qing Chang (93814), Huaju Sun (4449529), Kungang Chai (4581787), Zuqiang Huang (1846783), Zhenxia Zhao (2569945), Zhongxing Zhao (2569948)

{"title":"Ultrafast Screening of a Novel, Moderately Hydrophilic Angiotensin-Converting-Enzyme-InhibitorynPeptide, RYL, from Silkworm Pupa Using an Fe-Doped-Silkworm-Excrement-DerivednBiocarbon: Waste Conversion by Waste","authors":"Long Liu (390523), Yanan Wei (3768307), Qing Chang (93814), Huaju Sun (4449529), Kungang Chai (4581787), Zuqiang Huang (1846783), Zhenxia Zhao (2569945), Zhongxing Zhao (2569948)","doi":"10.1021/acs.jafc.7b04442.s001","DOIUrl":"https://doi.org/10.1021/acs.jafc.7b04442.s001","url":null,"abstract":"A novel, moderately hydrophilic peptiden(RYL) with high ACE-inhibitorynactivity was screened ultrafast via a concept of waste conversionnusing waste. This novel peptide was screened from silkworm pupa usingnan Fe-doped porous biocarbon (FL/Z-SE) derived from silkworm excrement.nFL/Z-SE possessed magnetic properties and specific selection for peptidesndue to Fe’s dual functions. The selected RYL, which has moderatenhydrophilicity (Log<i>P</i> = −0.22), exhibited ancomparatively high ACE-inhibitory activity (IC<sub>50</sub> = 3.31n± 0.11 μM). The inhibitory kinetics and docking-simulationnresults show that, as a competitive ACE inhibitor, RYL formed fivenhydrogen bonds with the ACE residues in the S1 and S2 pockets. Innthis work, both the screening carbon material and the selected ACE-inhibitorynpeptide were derived from agricultural waste (silkworm excrement andnpupa), which offers a new way of thinking about the development ofnadvanced uses of the silkworm byproducts and wastes.","PeriodicalId":522158,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147892645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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