FigsharePub Date : 2021-04-02DOI: 10.1021/acs.langmuir.1c00063.s005
Kyriacos Yiannacou (10292766), Veikko Sariola (6574847)
{"title":"Controlled Manipulation and Active Sorting of ParticlesnInside Microfluidic Chips Using Bulk Acoustic Waves and Machine Learning","authors":"Kyriacos Yiannacou (10292766), Veikko Sariola (6574847)","doi":"10.1021/acs.langmuir.1c00063.s005","DOIUrl":"https://doi.org/10.1021/acs.langmuir.1c00063.s005","url":null,"abstract":"Manipulation of cells,ndroplets, and particles via ultrasound withinnmicrofluidic chips is a rapidly growing field, with applications inncell and particle sorting, blood fractionation, droplet transport,nand enrichment of rare or cancerous cells, among others. However,ncurrent methods with a single ultrasonic transducer offer limitedncontrol of the position of single particles. In this paper, we demonstratenclosed-loop two-dimensional manipulation of particles inside closed-channelnmicrofluidic chips, by controlling the frequency of a single ultrasoundntransducer, based on machine-vision-measured positions of the particles.nFor the control task, we propose using algorithms derived from thenfamily of multi-armed bandit algorithms. We show that these algorithmsncan achieve controlled manipulation with no prior information on thenacoustic field shapes. The method learns as it goes: there is no neednto restart the experiment at any point. Starting with no knowledgenof the field shapes, the algorithms can (eventually) move a particlenfrom one position inside the chamber to another. This makes the methodnvery robust to changes in chip and particle properties. We demonstratenthat the method can be used to manipulate a single particle, threenparticles simultaneously, and also a single particle in the presencenof a bubble in the chip. Finally, we demonstrate the practical applicationsnof this method in active sorting of particles, by guiding each particlento exit the chip through one of three different outlets at will. Becausenthe method requires no model or calibration, the work paves the wayntoward the acoustic manipulation of microparticles inside unstructurednenvironments.","PeriodicalId":522100,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147891510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}