{"title":"Nanoassembliesnof Chitosan-Based Polyelectrolyte Complexesnas Nucleic Acid Delivery Systems","authors":"HusseinH. Genedy (19170679), Thierry Delair (1944982), Pierre Alcouffe (1532404), Agnès Crépet (9375740), Elodie Chatre (19170682), Khair Alhareth (13362869), Alexandra Montembault (1944979)","doi":"10.1021/acs.biomac.4c00054.s001","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c00054.s001","url":null,"abstract":"Nucleic acid delivery requires vectorization for protectionnfromnnucleases, preventing clearance by the reticuloendothelial system,nand targeting to allow cellular uptake. Nanovectors meeting the abovenspecifications should be safe for the patient, simple to manufacture,nand display long-term stability. Our nanovectors were obtained vianthe green process of polyelectrolyte complexation, carried out atn25 °C in water at a low shear rate using chitosan (a polycationicnbiocompatible polysaccharide of specific molar mass and acetylationndegree) and dextran sulfate as a polyanionic biocompatible polysaccharide.nThese complexes formed nanoassemblies of primary nanoparticles (20–35nnm) and maintained their colloidal stability for over 1 year at 25n°C. They could be steam sterilized, and a model nucleic acidncould be either encapsulated or surface adsorbed. A targeting agentnwas finally bound to their surface. This work serves as a proof ofnconcept of the suitability of chitosan-based polyelectrolyte complexesnas nanovectors by sequential multilayered adsorption of various biomacromolecules.","PeriodicalId":522041,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}