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{"title":"Osteogenic Differentiation of Bone Marrow-DerivednMesenchymal Stem Cells in Electrospun Silica Nonwoven Fabrics","authors":"Kazutoshi Iijima (1990999), Shohei Ishikawa (4583068), Kohei Sasaki (707904), Mineo Hashizume (2496820), Masaaki Kawabe (5703857), Hidenori Otsuka (2502940)","doi":"10.1021/acsomega.8b01139.s001","DOIUrl":"https://doi.org/10.1021/acsomega.8b01139.s001","url":null,"abstract":"Silicannonwoven fabrics (SNFs) with enough mechanical strengthnare candidates as implantable scaffolds. Culture of cells thereinnis expected to affect the proliferation and differentiation of thencells through cell–cell and cell–SNF interactions. Innthis study, we examined three-dimensional (3D) SNFs as a scaffoldnof mesenchymal stem cells (MSCs) for bone tissue engineering applications.nThe interconnected highly porous microstructure of 3D SNFs is expectednto allow omnidirectional cell–cell interactions, and the morphologicalnsimilarity of a silica nanofiber to that of a fibrous extracellularnmatrix can contribute to the promotion of cell functions. 3D SNFsnwere prepared by the sol–gel process, and their mechanicalnproperties were characterized by the compression test and rheologicalnanalysis. In the compression test, SNFs showed a compressive elasticnmodulus of over 1 MPa and a compressive strength of about 200 kPa.nThese values are higher than those of porous polystyrene disks usednfor in vitro 3D cell culture. In rheological analysis, the elasticnmodulus and fracture stress were 3.27 ± 0.54 kPa and 25.9 ±n8.3 Pa, respectively. Then, human bone marrow-derived MSCs were culturednon SNFs, and the effects on proliferation and osteogenic differentiationnwere evaluated. The MSCs seeded on SNF proliferated, and the thicknessnof the cell layer became over 80 μm after 14 days of culture.nThe osteogenic differentiation of MSCs on SNFs was induced by thenculture in the commercial osteogenic differentiation medium. The alkalinenphosphatase activity of MSCs on SNFs increased rapidly and remainednhigh up to 14 days and was much higher than that on two-dimensionalntissue culture-treated polystyrene. The high expression of <i>RUNX2</i> and intense staining by alizarin red s after differentiationnsupported that SNFs enhanced the osteogenic differentiation of MSCs.nFurthermore, permeation analysis of SNFs using fluorescein isothiocyanate-dextrannsuggested a sufficient permeability of SNFs for oxygen, minerals,nnutrients, and secretions, which is important for maintaining thencell viability and vitality. These results suggested that SNFs arenpromising scaffolds for the regeneration of bone defects using MSCs,noriginated from highly porous and elastic SNF characters.","PeriodicalId":522029,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}