FigsharePub Date : 2019-01-03DOI: 10.1021/acs.analchem.8b04638.s001
Wei Liu (20030), Anyi Chen (1583968), Shengkai Li (657419), Kanfu Peng (6172433), Yaqin Chai (1405669), Ruo Yuan (1405666)
{"title":"Perylene Derivative/LuminolnNanocomposite as a StrongnElectrochemiluminescence Emitter for Construction of an UltrasensitivenMicroRNA Biosensor","authors":"Wei Liu (20030), Anyi Chen (1583968), Shengkai Li (657419), Kanfu Peng (6172433), Yaqin Chai (1405669), Ruo Yuan (1405666)","doi":"10.1021/acs.analchem.8b04638.s001","DOIUrl":"https://doi.org/10.1021/acs.analchem.8b04638.s001","url":null,"abstract":"Innthis work, an electrochemiluminescent (ECL) biosensor was constructednon the basis of amino-modified 3,4,9,10-perylenetetracarboxylic dianhydride/luminoln(PTC-NH<sub>2</sub>/Lu) nanocomposite as emitter and bipedal DNA walkernsignal amplification strategy for ultrasensitive detection of microRNA-21n(miRNA-21). The PTC-NH<sub>2</sub>/Lu nanocomposite was prepared asnsignal tag via π–π stacking molecular assembly,nin which amino-modified 3,4,9,10-perylenetetracarboxylic dianhydriden(PTC-NH<sub>2</sub>) as a novel coreaction accelerator significantlynenhanced the ECL emission of luminol-H<sub>2</sub>O<sub>2</sub> system.nMoreover, target miRNA-21 triggered bipedal DNA walker was powerednby toehold-mediated strand displacement reaction (TSDR) for signalnamplification. Consequently, the proposed ECL biosensor achieved ultrasensitivendetection of miRNA-21 with a linear range from 100 aM to 100 pM andna limit of detection of 33 aM. Simultaneously, the biosensor was alsonsuccessfully applied to detect target miRNA-21 in lysates from humanncancer cells. As a result, this work constructed a new signal amplificationnplatform, exhibiting great application potential in biomedical analysisnand early clinical diagnostics.","PeriodicalId":522014,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}