FigsharePub Date : 2017-08-16DOI: 10.1021/acs.biomac.7b00677.s001
Dominic Keller (4356673), Ana Beloqui (156015), Mónica Martínez-Martínez (4356670), Manuel Ferrer (111944), Guillaume Delaittre (2084125)
{"title":"Nitrilotriacetic Amine-Functionalized Polymeric Core–ShellnNanoparticles as Enzyme Immobilization Supports","authors":"Dominic Keller (4356673), Ana Beloqui (156015), Mónica Martínez-Martínez (4356670), Manuel Ferrer (111944), Guillaume Delaittre (2084125)","doi":"10.1021/acs.biomac.7b00677.s001","DOIUrl":"https://doi.org/10.1021/acs.biomac.7b00677.s001","url":null,"abstract":"Nitrilotriaceticnamine (NTA)-functionalized nanoparticles obtainednby aqueous polymerization-induced self-assembly (PISA) are introducednas immobilization supports for polyhistidine-functionalized (His-tagged)nenzymes. A novel initiator for nitroxide-mediated polymerization basednon the nitroxide SG1 and carrying a protected NTA moiety was firstnsynthesized. Size-exclusion chromatography (SEC) and electrospraynionization mass spectrometry (ESI-MS) proved the ability of this initiatornto produce well-defined end-functional vinyl polymers. Subsequently,noligo(ethylene glycol) methacrylate-based macroinitiators were synthesizednand chain-extended to form amphiphilic block copolymer nanoparticles,neither by nanoprecipitation or by PISA. The latter method yieldednspherical nanoparticles with a higher definition, as demonstratednby dynamic light scattering (DLS). Deprotection of the NTA moietynand complexation with nickel ions were assessed by DLS and inductivelyncoupled plasma optical emission spectroscopy/mass spectrometry (ICP-OES/MS).nFinally, immobilization of His-tagged horseradish peroxidase and esternhydrolase were successfully carried out, leading to catalyticallynactive nanobiocatalysts, as shown by UV–vis measurements.","PeriodicalId":521545,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}