Figshare最新文献

筛选
英文 中文
Nitrilotriacetic Amine-Functionalized Polymeric Core–ShellnNanoparticles as Enzyme Immobilization Supports 硝化三乙酸胺功能化聚合物核壳纳米颗粒作为酶固定化载体
Figshare Pub Date : 2017-08-16 DOI: 10.1021/acs.biomac.7b00677.s001
Dominic Keller (4356673), Ana Beloqui (156015), Mónica Martínez-Martínez (4356670), Manuel Ferrer (111944), Guillaume Delaittre (2084125)
{"title":"Nitrilotriacetic Amine-Functionalized Polymeric Core–ShellnNanoparticles as Enzyme Immobilization Supports","authors":"Dominic Keller (4356673), Ana Beloqui (156015), Mónica Martínez-Martínez (4356670), Manuel Ferrer (111944), Guillaume Delaittre (2084125)","doi":"10.1021/acs.biomac.7b00677.s001","DOIUrl":"https://doi.org/10.1021/acs.biomac.7b00677.s001","url":null,"abstract":"Nitrilotriaceticnamine (NTA)-functionalized nanoparticles obtainednby aqueous polymerization-induced self-assembly (PISA) are introducednas immobilization supports for polyhistidine-functionalized (His-tagged)nenzymes. A novel initiator for nitroxide-mediated polymerization basednon the nitroxide SG1 and carrying a protected NTA moiety was firstnsynthesized. Size-exclusion chromatography (SEC) and electrospraynionization mass spectrometry (ESI-MS) proved the ability of this initiatornto produce well-defined end-functional vinyl polymers. Subsequently,noligo­(ethylene glycol) methacrylate-based macroinitiators were synthesizednand chain-extended to form amphiphilic block copolymer nanoparticles,neither by nanoprecipitation or by PISA. The latter method yieldednspherical nanoparticles with a higher definition, as demonstratednby dynamic light scattering (DLS). Deprotection of the NTA moietynand complexation with nickel ions were assessed by DLS and inductivelyncoupled plasma optical emission spectroscopy/mass spectrometry (ICP-OES/MS).nFinally, immobilization of His-tagged horseradish peroxidase and esternhydrolase were successfully carried out, leading to catalyticallynactive nanobiocatalysts, as shown by UV–vis measurements.","PeriodicalId":521545,"journal":{"name":"Figshare","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信
小红书