Glycoscience & therapy最新文献

{"title":"Synthesis of and characterization of differentially sulfated disaccharide repeating units of keratan sulfate.","authors":"Anupama Das, Gustavo A Kashiwagi, Qi Gan, Melanie L Shadrick, Adriana M Montaño, Alexei V Demchenko","doi":"10.1016/j.glycos.2025.100023","DOIUrl":"10.1016/j.glycos.2025.100023","url":null,"abstract":"<p><p>Keratan sulfate (KS) is a member of a broad family of sulfated polysaccharides known as glycosaminoglycans. Synthetic KS derivatives can lead to the advancement in early detection of mucopolysaccharidoses disorders using mass spectrometry. Reported herein is the synthesis of all four differentially sulfated keratan sulfate disaccharide repeat units. The efficient synthetic strategies to obtain non-sulfated, two different monosulfated, and disulfated derivatives has been developed. In accordance with our retrosynthetic analysis, all backbone disaccharide intermediates were synthesized from unique glycosyl donors and the universal glycosyl acceptor. Final purification of target compounds was accomplished by size exclusion chromatography, and all compounds were thoroughly characterized.</p>","PeriodicalId":521240,"journal":{"name":"Glycoscience & therapy","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147680264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycan Sequencing, A Brief Primer.","authors":"Xu Wang, David Mikhail","doi":"10.1016/j.glycos.2025.100022","DOIUrl":"10.1016/j.glycos.2025.100022","url":null,"abstract":"<p><p>Polysaccharides are among the most important biopolymers in nature. Besides playing an important structural role, polysaccharides in all organisms are vital modulators of cellular processes and control many physiological phenomena, including growth and proliferation, microbial infection, and inflammation. As a result, understanding the structure-function relationships of glycans is crucial. However, glycan sequencing technologies have fallen behind those of other biopolymers. This review discusses existing glycan sequencing methods for small- and medium-sized glycans, such as <i>N</i>-glycans, but will focus on upcoming methods for sequencing large glycans.</p>","PeriodicalId":521240,"journal":{"name":"Glycoscience & therapy","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12885369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146160102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A supramolecular self-assembly with dual functions of GSH-synthesis inhibition and -consumption for efficient enhancing chemodynamic therapy","authors":"Xiao Duan, Xihua Li, Caiping Liu, Xinping Liu, Yang Bai","doi":"10.1016/j.glycos.2025.100011","DOIUrl":"https://doi.org/10.1016/j.glycos.2025.100011","url":null,"abstract":"The excessive reduced GSH obviously restrict the chemodynamic therapy (CDT) efficacy through the consumption of ·OH. Therefore, the design of nano-systems to consume GSH is one effective method to enhance CDT efficacy. In this paper, β-cyclodextrin grafted hyaluronic acid (HA-CD) is utilized as host moieties to include ferrocene-arylboronic acid conjugate (Fc-BA) through host-guest inclusion, further binding curcumin to form supramolecular self-assemblies with active targeting ability and tumor micro-environment stimuli-responsive behavior. Fc could catalyze H 2 O 2 to generate ·OH through Fenton reaction, and curcumin could be released under acidic pH to realize the consumption of GSH and inhibition of thioredoxin reductase, reducing the biosynthesis of GSH. Based on this strategy, the over-expressed GSH levels in tumor can be effectively consumed to achieve satisfied CDT outcome. In vitro and in vivo experiments suggested that this GSH-synthesis inhibition and -consumption strategy can significantly amplify cellular oxidative stress for excellent CDT efficacy.","PeriodicalId":521240,"journal":{"name":"Glycoscience & therapy","volume":"1 1","pages":"100011-100011"},"PeriodicalIF":0.0,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endothelial neuraminidase 1 inhibition attenuates LPS-induced acute lung injury through the CXCR4 pathway","authors":"Yujie Wu, Si-Jia Jin, Qianqian Chen, Lian‐Wen Qi, Lei Zhang","doi":"10.1016/j.glycos.2025.100008","DOIUrl":"https://doi.org/10.1016/j.glycos.2025.100008","url":null,"abstract":"Neuraminidase 1 (NEU1), a key sialidase in human pulmonary microvascular endothelial cells, plays a central role in idiopathic pulmonary fibrosis. However, its contribution to endothelial dysfunction in acute lung injury (ALI) remains unclear. Notably, although natural NEU1 inhibitors have been initially identified, their specific molecular mechanisms and potential therapeutic effects in ALI remain to be elucidated- a critical research gap hindering the translation of NEU1-targeted therapies into clinical practice for ALI. We established a mouse lung infection model and a cultured endothelial cell model stimulated by LPS. NEU1 knockdown, overexpression, and pharmacological inhibition were employed to assess its impact on endothelial inflammation and dysfunction. The interaction between NEU1 and its target proteins, as well as the downstream signaling pathways, were analyzed using surface plasmon resonance (SPR), co-immunoprecipitation (Co-IP), Western blotting, and signaling pathway analysis. Based on previous research, baicalin was screened as potential inhibitors of NEU1, and their effects and potential mechanisms were analyzed through in vitro and in vivo studies. This study demonstrates that NEU1 expression is elevated in LPS-induced endothelial cells and lung tissue in ALI mice, suggesting that NEU1 may serve as a key pathogenic factor in ALI. NEU1 knockdown or pharmacological inhibition alleviates endothelial inflammation and dysfunction, while its overexpression promotes inflammatory gene transcription in human aortic endothelial cells. Mechanistically, NEU1 directly interacts with CXCR4 to enhance its protein stability, which in turn activates the JNK signaling pathway and contributes to endothelial dysfunction. Endothelial-specific NEU1 knockdown reduces CXCR4 expression and alleviates LPS-induced ALI. Baicalin, a flavonoid from Scutellaria baicalensis Georgi, binds to NEU1, promoting its lysosome-dependent degradation and inhibiting the NEU1/CXCR4 axis. In vivo experiments further confirmed that baicalin significantly attenuates lung injury and improves pulmonary function in ALI. Additionally, Huang-Lian-Jie-Du decoction (HLJDD), a traditional Chinese medicine containing baicalin, demonstrates therapeutic efficacy against ALI by targeting NEU1/CXCR4 and reducing inflammatory responses, providing new evidence supporting the clinical application of traditional Chinese medicine for ALI treatment. This study establishes NEU1 as a critical driver of ALI and identifies baicalin and HLJDD as promising therapeutic interventions, providing a new theoretical basis and potential therapeutic targets for the clinical treatment of ALI.","PeriodicalId":521240,"journal":{"name":"Glycoscience & therapy","volume":"1 1","pages":"100008-100008"},"PeriodicalIF":0.0,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}