Frontiers in imaging (Lausanne, Switzerland)最新文献

{"title":"Novel imaging approach for simultaneous tracking of cell dynamics in distinct tissue layers reveals cells involved in colonic peristalsis.","authors":"Salah A Baker, Peter J Blair, Sharif Amit Kamran, Kenton M Sanders","doi":"10.3389/fimag.2025.1538533","DOIUrl":"10.3389/fimag.2025.1538533","url":null,"abstract":"<p><p>We have developed a novel approach for high-resolution confocal imaging across multiple tissue planes simultaneously. By combining confocal microscopy, piezo actuators, and optogenetic sensors, we can simultaneously capture images of dynamic fluorescence signals from various cell populations in different tissue layers (Z planes). This enables the decoding of cell-to-cell communication through complex tissues, offering a significant advancement in understanding how cells in distinct layers of tissue communicate and coordinate their functions and produce integrated behaviors. For example, our technique sheds light on myogenic coordination underlying colonic motility. Examining various cell types, such as interstitial cells of Cajal (ICC) and smooth muscle cells (SMC), distributed through the thickness of muscle layers, we demonstrate distinct Ca²⁺ signaling patterns and organization that underlie complex colonic motor activities.</p>","PeriodicalId":521217,"journal":{"name":"Frontiers in imaging (Lausanne, Switzerland)","volume":"4 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12834056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146069810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Independent Morphometric Machine Learning Workflow for Single-Cell Antimicrobial Susceptibility Testing of <i>Klebsiella pneumoniae</i> to Meropenem.","authors":"Kristel C Tjandra, Nikhil Ram-Mohan, Manuel Roshardt, Elizabeth Zudock, Zhaonan Qu, Kathleen E Mach, Okyaz Eminaga, Joseph C Liao, Samuel Yang, Pak Kin Wong","doi":"10.3389/fimag.2024.1418669","DOIUrl":"10.3389/fimag.2024.1418669","url":null,"abstract":"<p><p>Multidrug-resistant <i>Enterobacteriaceae</i> are among the most urgent global public health threats associated with various life-threatening infections. In the absence of a rapid method to identify antimicrobial susceptibility, empirical use of broad-spectrum antimicrobials such as carbapenem monotherapy has led to the spread of resistant organisms. Rapid determination of antimicrobial resistance is urgently needed to overcome this issue. By capturing dynamic single-cell morphological features, including growth-independent, antibiotic-induced changes, of cells from nineteen strains of <i>Klebsiella pneumoniae</i>, we evaluated strategies based on time and concentration differentials for classifying its susceptibility to a commonly used carbapenem, meropenem, and predicting their minimum inhibitory concentrations (MIC). We report morphometric antimicrobial susceptibility testing (MorphoAST), a growth independent, computer vision-based machine learning workflow, for rapid determination of antimicrobial susceptibility by single-cell morphological analysis within sub-doubling time of <i>K. pneumoniae</i>. We demonstrated the technological feasibility of predicting MIC/antimicrobial susceptibility in a fraction of the bacterial doubling time (<50 min.). The classifiers achieved as high as 97% accuracy in 20 minutes (two-fifths of the doubling time) and reached over 99% accuracy within 50 minutes (one doubling time) in predicting the antimicrobial response of the validation dataset. A regression model based on the concentration differential of individual cells from nineteen strains predicted the MIC with 100% categorical agreement and essential agreement for seven unseen strains, including two clinical samples from patients with urinary tract infections with different responsiveness to meropenem, within 50 minutes of treatment. The expansion of this innovation to other drug-bug combinations could have significant implications for the future development of rapid antimicrobial susceptibility testing.</p>","PeriodicalId":521217,"journal":{"name":"Frontiers in imaging (Lausanne, Switzerland)","volume":"3 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13618438/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149077519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}