Jingwei Zhao, Yongjun Kim, Momoka Sugimura, Kenneth Marcelino, Rafael Romero, Brooke Liang, Michelle Khan, Eric Yang, Dongkyun Kang
{"title":"Compact scattering-based light sheet microscopy probe using a custom miniature objective lens.","authors":"Jingwei Zhao, Yongjun Kim, Momoka Sugimura, Kenneth Marcelino, Rafael Romero, Brooke Liang, Michelle Khan, Eric Yang, Dongkyun Kang","doi":"10.1117/1.jom.4.3.034501","DOIUrl":"10.1117/1.jom.4.3.034501","url":null,"abstract":"<p><p>We have developed a compact scattering-based light sheet microscopy (sLSM) probe capable of imaging unstained tissues with cellular resolution. In the compact sLSM probe, a custom miniature objective lens was developed to achieve a high lateral resolution, large field of view (FOV), and small field curvature. The measured resolution of the custom objective was 1.65-1.97 μm across the FOV of ± 1.0 mm. The compact probe had dimensions of 4 cm in width and height, and 10 cm in length. The compact sLSM probe achieved an axial resolution better than 5.6 μm over a depth range of 206.2 μm, and a lateral resolution of 1.9 μm. Preliminary results showed that the compact sLSM probe could visualize cellular details of fixed human anal epithelial tissues in a similar manner to a bench LSM device using off-the-shelf objective lenses.</p>","PeriodicalId":521084,"journal":{"name":"Journal of optical microsystems","volume":"4 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12490782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145234902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}