International journal of high speed electronics最新文献

{"title":"FIBER DIAMETER-DRIVEN MODULATION OF CELL BEHAVIOR IN DECM-ENRICHED ELECTROSPUN SCAFFOLDS FOR BONE TISSUE ENGINEERING.","authors":"Sai Sadhananth Srinivasana, Suranji Wijekoona, Allen Zennifera, Sama Abdulmalika, Sangamesh G Kumbara","doi":"10.1142/S0129156426400124","DOIUrl":"10.1142/S0129156426400124","url":null,"abstract":"<p><p>Effective regeneration of large bone defects remains a major clinical challenge, as current bone graft substitutes often result in peripheral mineralization without uniform tissue regeneration across the defect. While mechanical requirements can be addressed through appropriate material selection and scaffold design, many grafts lack the bioactivity needed for consistent and robust bone formation. This study presents a strategy to enhance graft bioactivity using cell-laden extracellular matrix (ECM) as a bioactive component. Our objective is to culture human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on a nanofiber matrix, then isolate the cell-laden extracellular matrix (ECM) and decellularize it to obtain osteogenic dECM. This study investigates how PCL fiber diameter affects hMSC attachment, proliferation, and osteogenic differentiation for use in dECM collection. PCL fibers in the 200-400 nm range promoted hMSCs proliferation, alkaline phosphatase (ALP) activity, and mineralization under osteoinduction. Due to the hydrophobic nature of PCL, cell sheets detached easily, indicating poor cell-material interaction. To address this, cellulose acetate (CA) was blended with PCL at an 80:20 wt.% ratio to form nanofibers (NF) with optimal diameters. These blended NFs were used to culture hMSCs, HUVECs, or both under osteoinductive conditions for 21 days to harvest cell-laden ECM. After decellularization, three ECM-coated groups were generated: NF+H (HUVECs), NF+M (hMSCs), and NF+Hyb (co-culture). The bioactivity of the cell-laden dECM matrices, particularly NF+Hyb, significantly enhanced the osteogenic activity of cultured hMSCs compared to other groups. This strategy offers a promising approach to boost bone graft bioactivity without relying on exogenous growth factors.</p>","PeriodicalId":520499,"journal":{"name":"International journal of high speed electronics","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13089772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-Nanostructured Polymeric Scaffolds for Bone Tissue Engineering.","authors":"Sama Abdulmalik, Suranji Wijekoon, Khadija Basiru Danazumi, Sai Sadhananth Srinivasan, Laxmi Vobbineni, Elifho Obopilwe, Sangamesh G Kumbar","doi":"10.1142/s0129156424400755","DOIUrl":"10.1142/s0129156424400755","url":null,"abstract":"<p><p>While bone tissue allograft and autograft are commonly used in bone healing, their application is limited by factors such as availability, donor site morbidity, and immune response to the grafted tissue. Tissue-engineered implants, such as acellular or cellular polymeric structures, offer a promising alternative, and are a current trend in tissue engineering. Leveraging recent advancements in bone tissue engineering (BTE), we utilize 3D printing to develop biodegradable scaffolds that combine mechanical strength and bioactivity to facilitate bone repair and regeneration. This study focuses on the design and fabrication of mechanically competent 3D printed poly (L-lactic acid) (PLLA) micro-structured scaffolds. These scaffolds are enhanced with collagen type I nanofibrils to create bioactive scaffolds that promote tissue regeneration. The performance of these mechanically competent, micro-nanostructured polymeric matrices, in combination with bone marrow stromal cells (BMSCs), is evaluated in PLLA and PLLA-collagen scaffolds. The resulting micro-nanostructured PLLA-Collagen scaffolds mimic trabecular bone architecture, mechanical strength, and the extracellular matrix environment found in native bone tissue. The composite PLLA-collagen scaffolds exhibit mechanical properties in the mid-range of human trabecular bone. Both PLLA and PLLA-Collagen scaffolds support human BMSCs adhesion, proliferation, and osteogenic differentiation. A significantly higher number of implanted host cells are distributed in the PLLA-Collagen scaffolds with greater bone density, more uniform cell distribution, and attachment compared to the PLLA microstructure. Additionally, the biomimetic collagen nanostructure potently induces osteogenic transcription evidenced by increased alkaline phosphatase activity and upregulation of bone markers such as sialoprotein and collagen type I, ultimately guiding stem cell-mediated formation of a mature, mineralized bone matrix throughout the interconnected scaffold pores. This study underscores the benefits of micro-nanostructured scaffolds in successfully generating the inductive microenvironment of native bone extracellular matrix, triggering the cascade of cellular events required for functional bone regeneration, repairing critical-sized bone defects, and ultimately serving as an alternative material platform for bone regeneration, thereby instilling confidence in the potential of our research.</p>","PeriodicalId":520499,"journal":{"name":"International journal of high speed electronics","volume":"33 2-3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}