Gout, urate, and crystal deposition disease最新文献

筛选
英文 中文
A Novel Polarized Light Microscope for the Examination of Birefringent Crystals in Synovial Fluid. 一种用于检查滑液双折射晶体的新型偏振光显微镜。
Gout, urate, and crystal deposition disease Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI: 10.3390/gucdd2040022
John D FitzGerald, Chesca Barrios, Tairan Liu, Ann Rosenthal, Geraldine M McCarthy, Lillian Chen, Bijie Bai, Guangdong Ma, Aydogan Ozcan
{"title":"A Novel Polarized Light Microscope for the Examination of Birefringent Crystals in Synovial Fluid.","authors":"John D FitzGerald, Chesca Barrios, Tairan Liu, Ann Rosenthal, Geraldine M McCarthy, Lillian Chen, Bijie Bai, Guangdong Ma, Aydogan Ozcan","doi":"10.3390/gucdd2040022","DOIUrl":"10.3390/gucdd2040022","url":null,"abstract":"<p><strong>Background: </strong>The gold standard for crystal arthritis diagnosis relies on the identification of either monosodium urate (MSU) or calcium pyrophosphate (CPP) crystals in synovial fluid. With the goal of enhanced crystal detection, we adapted a standard compensated polarized light microscope (CPLM) with a polarized digital camera and multi-focal depth imaging capabilities to create digital images from synovial fluid mounted on microscope slides. Using this single-shot computational polarized light microscopy (SCPLM) method, we compared rates of crystal detection and raters' preference for image.</p><p><strong>Methods: </strong>Microscope slides from patients with either CPP, MSU, or no crystals in synovial fluid were acquired using CPLM and SCPLM methodologies. Detection rate, sensitivity, and specificity were evaluated by presenting expert crystal raters with (randomly sorted) CPLM and SCPLM digital images, from FOV above clinical samples. For each FOV and each method, each rater was asked to identify crystal suspects and their level of certainty for each crystal suspect and crystal type (MSU vs. CPP).</p><p><strong>Results: </strong>For the 283 crystal suspects evaluated, SCPLM resulted in higher crystal detection rates than did CPLM, for both CPP (51%. vs. 28%) and MSU (78% vs. 46%) crystals. Similarly, sensitivity was greater for SCPLM for CPP (0.63 vs. 0.35) and MSU (0.88 vs. 0.52) without giving up much specificity resulting in higher AUC.</p><p><strong>Conclusions: </strong>Subjective and objective measures of greater detection and higher certainty were observed for SCPLM over CPLM, particularly for CPP crystals. The digital data associated with these images can ultimately be incorporated into an automated crystal detection system that provides a quantitative report on crystal count, size, and morphology.</p>","PeriodicalId":520386,"journal":{"name":"Gout, urate, and crystal deposition disease","volume":"2 4","pages":"315-324"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143019984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信