Epigenetics reports最新文献

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Maternal Diet Quality in Pregnancy and Human Milk Extracellular Vesicle and Particle microRNA. 孕妇饮食质量与母乳细胞外囊泡和微粒microRNA。

Epigenetics reports Pub Date : 2025-01-01 Epub Date: 2025-05-30 DOI: 10.1080/28361512.2025.2508883

Meghan E Muse, Yuting Wang, Diane Gilbert-Diamond, David A Armstrong, Anne G Hoen, Megan E Romano, Jiang Gui, Thomas J Palys, Frederick W Kolling, Brock C Christensen, Margaret R Karagas, Caitlin G Howe

{"title":"Maternal Diet Quality in Pregnancy and Human Milk Extracellular Vesicle and Particle microRNA.","authors":"Meghan E Muse, Yuting Wang, Diane Gilbert-Diamond, David A Armstrong, Anne G Hoen, Megan E Romano, Jiang Gui, Thomas J Palys, Frederick W Kolling, Brock C Christensen, Margaret R Karagas, Caitlin G Howe","doi":"10.1080/28361512.2025.2508883","DOIUrl":"10.1080/28361512.2025.2508883","url":null,"abstract":"Extracellular vesicle and particle microRNAs (EVP miRNA) in milk have the capacity to facilitate maternal-infant communication in the postpartum period and are hypothesized to play important roles in child development. Maternal diet quality has been linked to milk macronutrient composition, microbiota profiles, as well as various child health outcomes. The aim of this study was to assess the association between maternal diet quality and milk EVP miRNA. In a pilot study of 54 participants from a larger birth cohort study, diet quality was measure by the Alternative Healthy Eating Index 2010 (AHEI-2010) during the second trimester of pregnancy and 798 EVP miRNA were profiled in mature milk samples (collected approximately six weeks postpartum) using the NanoString nCounter platform. In covariate-adjusted models, AHEI-2010 was positively associated (Q < 0.05) with levels of miR-1283, miR-520h, and mir-570-3p in milk EVPs. Predicted target genes of these diet-associated miRNA are enriched in pathways related to lactation and mammary development (PI3 kinase signaling and Wnt signaling pathways) and milk protein and fat synthesis (PI3 kinase signaling). Further research is needed to investigate whether these diet-associated miRNA influence lactation, human milk quality, and child growth and development.","PeriodicalId":520309,"journal":{"name":"Epigenetics reports","volume":"3 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144593739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterizing DNA Methylation and Hydroxymethylation in Cord Blood and Identifying Sex-Specific Differences using the Illumina EPIC Array. 使用Illumina EPIC阵列表征脐带血DNA甲基化和羟甲基化并鉴定性别特异性差异。

Epigenetics reports Pub Date : 2024-01-01 Epub Date: 2024-11-14 DOI: 10.1080/28361512.2024.2427955

Rebekah L Petroff, Dana C Dolinoy, Vasantha Padmanabhan, Jaclyn M Goodrich

{"title":"Characterizing DNA Methylation and Hydroxymethylation in Cord Blood and Identifying Sex-Specific Differences using the Illumina EPIC Array.","authors":"Rebekah L Petroff, Dana C Dolinoy, Vasantha Padmanabhan, Jaclyn M Goodrich","doi":"10.1080/28361512.2024.2427955","DOIUrl":"10.1080/28361512.2024.2427955","url":null,"abstract":"DNA methylation, an epigenetic mark, has become a common outcome in epidemiological studies with the aid of affordable and reliable technologies. Yet the most widespread technique used to assess methylation, bisulfite conversion, does not allow for the differentiation of regular DNA methylation (5-mC) and other cytosine modifications, like that of hydroxymethylation (5-hmC). As both 5-mC and 5-hmC have distinct biological roles, sometimes with opposing effects, it is crucial to understand the difference between these marks. To characterize 5-mC and 5-hmC in cord blood and expand on previously published results in smaller cohorts, 73 samples from infants in the Michigan Mother Infant Pairs cohort were paired bisulfite and oxidative bisulfite converted. 5-mC and 5-hmC were assessed on the Illumina Infinium EPIC array, using maximum likelihood methods, and sex-specific differences of these marks were analyzed. 5-mC and 5-hmC were both broadly distributed across the genome, and 5-hmC was prevalent, with proportions of 0.01-0.55. Sex-specific analysis revealed total methylation was different on 17,000 sites (q<0.05), but only different at 1,866 and 5 sites of 5-mC and 5-hmC specifically. These results add additional support to the literature and demonstrate the importance of differentiating between 5-mC and 5-hmC in epidemiological studies going forward.","PeriodicalId":520309,"journal":{"name":"Epigenetics reports","volume":"2 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142752778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0