Ziyan Wang, Kristin M Lindgren, Jennifer J Loros, Jay C Dunlap
{"title":"The auxotrophic <i>formate</i> (<i>for</i>) mutant of <i>Neurospora crassa</i> has significantly delayed growth but a normal circadian clock.","authors":"Ziyan Wang, Kristin M Lindgren, Jennifer J Loros, Jay C Dunlap","doi":"10.4148/1941-4765.2185","DOIUrl":"10.4148/1941-4765.2185","url":null,"abstract":"<p><p>Some cell biological studies of <i>Neurospora crassa</i> have been limited by the rapid rates of hyphal growth and fusion. In this study, we investigated the causative mutation in the standard C24 allele of <i>for</i> (FGSC #9) and assayed the growth and circadian phenotype of the <i>for</i> strain under different nutritional conditions. We show that the <i>for</i> strain can be maintained as metabolically active single cells for 2 days before its growth advances into branched mycelia. This culturing system offers the potential to advance subcellular dynamic research and to facilitate greater understanding of <i>N. crassa</i> in the early developmental stages.</p>","PeriodicalId":520293,"journal":{"name":"Fungal genetics reports","volume":"68 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142650356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}