{"title":"Detection of environmental pollutants using transcription factor-based whole-cell biosensors.","authors":"Yuan Dou, Jianli Zhang, Xinyu Gong, Qi Gan, Shuo Yu, Yajun Yan","doi":"10.1186/s44314-025-00032-7","DOIUrl":"10.1186/s44314-025-00032-7","url":null,"abstract":"<p><p>The widespread presence of heavy metals and aromatic compounds in environmental systems has emerged as a critical global concern. While conventional analytical methods such as spectroscopy and chromatography provide high precision, their substantial costs and labor-intensive procedures significantly limit their practical application in routine environmental monitoring. Transcription factor-based whole-cell biosensors represent a promising alternative technology, providing real-time, highly sensitive, and cost-effective detection capabilities for environmental pollutants. This review is a comprehensive examination of the recent advances in transcription factor-based biosensors for detecting heavy metals and aromatic compounds. We also emphasize the molecular mechanisms of diverse TF families and their real-world applications in environmental monitoring. Finally, we summarize the current engineering strategies that are employed to enhance biosensor performance and discuss future directions for improving transcription factor-based biosensors for environmental detection.</p>","PeriodicalId":519986,"journal":{"name":"Biotechnology for the environment","volume":"2 1","pages":"16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12665635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Investigation of medicinal plant processing waste for electricity generation through biomethanation","authors":"Kartikeya Nayak, V. Kumargouda, Kavan Kumar","doi":"10.1186/s44314-024-00004-3","DOIUrl":"https://doi.org/10.1186/s44314-024-00004-3","url":null,"abstract":"","PeriodicalId":519986,"journal":{"name":"Biotechnology for the environment","volume":" 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141833483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engineered plastic-associated bacteria for biodegradation and bioremediation.","authors":"Arianna Schneier, Gavin Melaugh, Joanna C Sadler","doi":"10.1186/s44314-024-00007-0","DOIUrl":"10.1186/s44314-024-00007-0","url":null,"abstract":"<p><p>The global plastic waste crisis has triggered the development of novel methods for removal of recalcitrant polymers from the environment. Biotechnological approaches have received particular attention due to their potential for enabling sustainable, low-intensity bioprocesses which could also be interfaced with microbial upcycling pathways to support the emerging circular bioeconomy. However, low biodegradation efficiency of solid plastic materials remains a bottleneck, especially at mesophilic conditions required for one-pot degradation and upcycling. A promising strategy used in nature to address this is localisation of plastic-degrading microbes to the plastic surface via biofilm-mediated surface association. This review highlights progress and opportunities in leveraging these naturally occurring mechanisms of biofilm formation and other cell-surface adhesion biotechnologies to co-localise engineered cells to plastic surfaces. We further discuss examples of combining these approaches with extracellular expression of plastic-degrading enzymes to accelerate plastic degradation. Additionally, we review this topic in the context of nano- and microplastics bioremediation and their removal from wastewater and finally propose future research directions for this nascent field.</p>","PeriodicalId":519986,"journal":{"name":"Biotechnology for the environment","volume":"1 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11256910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141725478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}