Current Biochemistry最新文献

{"title":"Kandungan Nutrisi dan Daya Inhibisi α-glukosidase Ekstrak Daging Buah Salak Sidempuan (Salacca sumatrana)","authors":"Mega Safithri, Aprilita Putri Defan Ritonga, Susi Indariani, Maheswari Alfira Dwicesaria","doi":"10.29244/cb.11.1.1","DOIUrl":"https://doi.org/10.29244/cb.11.1.1","url":null,"abstract":"Penelitian terhadap pemanfaatan salak khususnya salak Sidempuan sebagai inhibitor α-glukosidase belum dilakukan. Penelitian ini bertujuan mengukur kadar proksimat (air, abu, protein kasar, lemak kasar, serat kasar, dan karbohidrat), menganalisis komponen fitokimia, kandungan total fenolik dan aktivitas penghambatan α-glukosidase pada ekstrak air dan etanol daging buah salak Sidempuan. Kadar total fenol diukur menggunakan metode Folin Ciocalteu. Aktivitas penghambatan α-glukosidase diukur menggunakan substrat pNPG. Kadar karbohidrat, air, abu, lemak kasar, protein kasar, dan serat kasar berturut-turut sebesar 86.35%, 6.57%, 3.60%, 1.44%, 1.24%, dan 0.82%. Kedua ekstrak mengandung senyawa flavonoid dan saponin. Ekstrak etanol 70% daging buah salak Sidempuan memiliki kandungan total fenol sebesar 10.6 mg GAE/g ekstrak, lebih tinggi dibandingkan ekstrak air sebesar 5.55 mg GAE/g esktrak. Ekstrak etanol daging buah salak Sidempuan memiliki konsentrasi inhibisi (IC50) sebesar 13.69 mg/L. Konsentrasi ini lebih kecil dibandingkan ekstrak air yaitu 4160.56 mg/L, namun masih lebih besar dibandingkan akarbosa yaitu 1.15x10-3 mg/L sebagai kontrol positif.","PeriodicalId":519054,"journal":{"name":"Current Biochemistry","volume":" 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"INHIBISI ENZIM α-GLUKOSIDASE OLEH SENYAWA FLAVONOID DAUN KELOR (Moringa oleifera) IN SILICO DAN IN VITRO","authors":"Shobiroh Nuur' Alimah, P. Puspita, L. Ambarsari, Riksa Nur Wahyuni","doi":"10.29244/cb.10.2.3","DOIUrl":"https://doi.org/10.29244/cb.10.2.3","url":null,"abstract":"Moringa leaves flavonoid have potential to inhibit α-glucosidase but they have low bioavailability, so they aremade in nanoparticles. It is also not known which specific flavonoid compounds from Moringa leaves have thepotential to inhibit α-glucosidase. This research aimed to determine the inhibition potential of α-glucosidase bythe moringa leaves flavonoid in silico through molecular docking and determine the inhibitory activity of α-glucosidase by moringa leaves flavonoid in extracts and nanoparticles in vitro. Moringa leaves flavonoid havepotential to be a competitive inhibiton of α-glucosidase with the highest to lowest inhibitory potential arecryptochlorogenic acid, quercetin-3-O-beta-D-glucopyranoside, quercetin-3-glucoside, kaempferol-3-O-a-ramnoside, kaempferol-3-O-glucoside, epicathechins, catechins, quercetin, kaempferol, glucomoringinisothiocyanate. Cryptochlorogenic acid has the best potential with ΔG and Ki values -8.5 kcal/mol and 0.5788μM. Inhibition α-glucosidase moringa leaves flavonoid in extract and nanoparticles respectively are classified asinactive (IC 50 = 5.84x10 3 ppm) and active (IC 50 = 1.59x10 1 ppm) in vitro, so nanoparticles can increase theinhibitory activity.","PeriodicalId":519054,"journal":{"name":"Current Biochemistry","volume":"30 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140970975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Bay Leaf Decoction (Syzygium polyanthum) on Reducing Blood Glucose Levels in Paok Motong, Masbagik, East Lombok","authors":"Nadi Kurniawan, Rozikin, I Putu Bayu Agus Saputra, Sabariah, I Nyoman Bagus Aji Kresnapati","doi":"10.29244/cb.10.2.2","DOIUrl":"https://doi.org/10.29244/cb.10.2.2","url":null,"abstract":"One disease that correlates with blood glucose levels is diabetes mellitus (DM). According to Basic Health Research (RISKESDAS), in Indonesia by 2030 there will be an increase of up to 21.3 million people suffering from diabetes mellitus. However, nowadays most people are afraid of the side effects of using the drugs they consume and are turning to herbal therapy to lower blood glucose levels. Bay leaves have the benefit of treating diabetes mellitus, hypercholesterolemia, hypertension, diarrhea and gastritis. Phytochemical analysis shows that bay leaves contain essential oils, tannins, flavonoids and terpenoids. The aim of the research was to determine the effect of bay leaf boiled water on reducing blood glucose levels. The research was an experimental One Group Pretest Post-test Design, namely the research subjects had their blood glucose levels measured before (pre-test) and after being given bay leaf boiled water (post-test). 41 respondents in Paok Motong Barat village, Masbagik District, East Lombok Regency had their Blood Glucose Levels (GDS) measured before (pre-test) and after (post-test) giving 300 mL of bay leaf boiled water. Paired Test Statistical Analysis was used to determine the difference in pretest and posttest blood glucose levels given bay leaf boiled water. The results of the study showed that there was a significant decrease in blood glucose levels (p= 0.001) by 15.22 mg/dL to 179.27 mg/dL before administering bay leaf decoction amounting to 194.49 mg/dL. The conclusion is that there is a significant effect (p=0.001) of giving boiled bay leaves on reducing blood glucose levels. The suggestion for this research is to increase the number of respondents in the research as well as examination variables such as fasting blood glucose","PeriodicalId":519054,"journal":{"name":"Current Biochemistry","volume":"64 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140529185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coagulation and Antibacterial Activity of Chicken Eggshell Hydrogel and Button Fungus Ethanol Extract as a Diabetic Wound Healer","authors":"Akbar Rafiqi, Alivianisa Nabilatusyifa, Oyi Candra Santoso Putri, Ikromatul Hasanah, Sabrina Firdayani Rahmah, Dimas Andrianto","doi":"10.29244/cb.10.1.5","DOIUrl":"https://doi.org/10.29244/cb.10.1.5","url":null,"abstract":"Diabetic patients are susceptible to severe wound conditions due to high blood sugar levels and bacterial infections. This study aims to determine the formulation and effectiveness of the hydrogel plaster combination of laying hen eggshells with button mushrooms in curing diabetic wounds. The research methods consisted of making the hydrogel, coagulation test, and antibacterial tests. Hydrogels were made with 5%, 10%, and 20% eggshell concentrations. The coagulation activity test used rabbit blood, which was added with glucose. Antibacterial test was performed using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This research shows that the coagulation activity of F2 and F3 formulations significantly differed from the control, with an average blood clotting time of 64.67 minutes and 63.00 minutes. The antibacterial test results produced a clear zone for S. aureus and E. coli with diameters of 10.27 mm and 9.18 mm for F2, 6.90 mm, and 11.66 mm for F3. This research concludes that hydrogel formulation 3, with 20% eggshell and 6.4% button mushroom, had the best blood clotting effect and antibacterial activity.","PeriodicalId":519054,"journal":{"name":"Current Biochemistry","volume":"278 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140531003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efektivitas Pemberian Air Rebusan Daun Salam (Syzygium Polyanthum) Terhadap Penurunan Kadar Glukosa Darah Di Daerah Paok Motong, Kecamatan Masbagik, Lombok Timur Effectiveness of Bay Leaf Decoction (Syzygium Polyanthum) on Reducing Blood Glucose Levels i","authors":"Aji Kresnapati, Rozikin, I Putu Bayu Agus Saputra, Sabariah, I Nyoman Bagus Aji Kresnapati","doi":"10.29244/cb.10.2.1","DOIUrl":"https://doi.org/10.29244/cb.10.2.1","url":null,"abstract":"One of the diseases related to blood glucose levels is diabetes mellitus (DM). According to Riset Kesehatan Dasar (RISKESDAS) in Indonesia, in 2030 there will be an increase of up to 21.3 million people suffering from diabetes mellitus. However, nowadays most people are afraid of the side effects of using the drugs they consume and are turning to herbal therapy to lower blood sugar levels. Bay leaves have many benefits, namely they can treat diabetes mellitus, high cholesterol, hypertension, diarrhea and gastritis. Phytochemical analysis shows that bay leaves contain essential oils, tannins, flavonoids and terpenoids. The research aims to determine the effectiveness of boiled bay leaves in reducing blood glucose levels. 41 respondents in the Paok Motong Barat area, East Lombok had their blood sugar levels measured (GDS) before (pretest) and after (posttest) administration of boiled bay leaves. Paired Test statistics were used to determine the difference in pretest and posttest blood glucose levels given bay leaf decoction. There was a significant decrease in blood sugar levels (p= 0.001) after administering 15.22 mg/dL of bay leaf decoction from 194.49 mg/dL before administration and 179.27 mg/dL after administration. The conclusion is that there is significant effectiveness (p=0.001) of giving boiled bay leaves in reducing blood glucose levels. \u0000Salah satu penyakit yang terkait dengan kadar glukosa darah adalah diabetes mellitus (DM). Menurut Riset Kesehatan Dasar (RISKESDAS) di Indonesia pada tahun 2030 akan mengalami peningkatan hingga 21,3 juta orang menderita diabetes mellitus. Namun sekarang ini kebanyakan masyarakat takut akan efek samping dari penggunaan obat yang dikonsumsi dan beralih terapi herbal untuk menurunkan kadar gula darah. Daun salam memiliki banyak manfaat yaitu dapat mengobati diabetes mellitus, kolesterol tinggi, hipertensi, diare, serta gastritis. Analisis fitokimia menunjukkan bahwa di dalam daun salam terdapat kandungan minyak esensial, tannin, flavonoid, dan terpenoid. Penelitian ini bertujuan untuk mengetahui efektivitas air rebusan daun salam terhadap penurunan kadar glukosa darah. 41 responden di daerah Paok Motong Barat, Lombok Timur di ukur kadar Gula Darah Sewaktu (GDS) sebelum (pre-test) dan sesudah (post-tets) pemberian air rebusan daun salam. Analisis Statisktik Paired Test digunakan untuk mengetahui perbedaan kadar glukosa darah pretest dan postest pemberian air rebusan daun salam. Hasil penelitian terjadi penurunan kadar gula darah secara signifikan (p= 0,001) setelah pemberian rebusan daun salam 15,22 mg/dL dari sebelum pemberian 194,49 mg/dL dan sesudah  179,27 mg/dL. Kesimpulan penelitian terdapat efektivitas secara signifikan (p=0,001) pemberian rebusan daun salam terhadap penurunan kadar glukosa darah. \u0000 ","PeriodicalId":519054,"journal":{"name":"Current Biochemistry","volume":"62 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140530926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}