发布求助
文献互助 智能选刊 最新文献

Journal of Biotechnology Research最新文献

筛选
英文 中文
Detection and Analysis of Quality Traits of Different Black Rice Varieties in the Southern Henan Rice Region 豫南稻区不同黑米品种品质性状的检测与分析
Journal of Biotechnology Research Pub Date : 2024-05-18 DOI: 10.32861/jbr.103.30.40
Wan-Ru Zhang, Qiang Zhao, Hao-Jia He, et. al.
{"title":"Detection and Analysis of Quality Traits of Different Black Rice Varieties in the Southern Henan Rice Region","authors":"Wan-Ru Zhang, Qiang Zhao, Hao-Jia He, et. al.","doi":"10.32861/jbr.103.30.40","DOIUrl":"https://doi.org/10.32861/jbr.103.30.40","url":null,"abstract":"Using 14 black rice varieties imported from Southern Henan as experimental materials, the appearance quality, grinding quality, nutritional quality, cooking and eating quality of grain in Southern Henan were tested and analyzed by means of rice huller, rice mopping machine and near-infrared grain analyzer, combined with biochemical and physical analysis techniques. The results showed that there were abundant variation types in grain length, grain width, length-to-width ratio, brown rice rate, milled rice rate, head rice rate, soluble protein content, starch content, gelatinization temperature, gel consistency and viscosity of black rice. Particularly, grain type, starch content, gelatinization temperature, viscosity and other important quality traits in black rice materials have a large variation range. Therefore, our results provide important clues for developing new high-quality varieties of black rice.","PeriodicalId":511904,"journal":{"name":"Journal of Biotechnology Research","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141125649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Determination of Resistance Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin and Wound 从皮肤和伤口分离的耐甲氧西林金黄色葡萄球菌（MRSA）耐药基因的分子测定
Journal of Biotechnology Research Pub Date : 2024-03-14 DOI: 10.32861/jbr.102.26.29
Diso S.U., Mukhtar M. D., Dutsinma U. A.
{"title":"Molecular Determination of Resistance Gene in Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Skin and Wound","authors":"Diso S.U., Mukhtar M. D., Dutsinma U. A.","doi":"10.32861/jbr.102.26.29","DOIUrl":"https://doi.org/10.32861/jbr.102.26.29","url":null,"abstract":"The wide spread use of antibiotic resulted in the development of resistance to antibiotics through acquisition of the mobile cassette chromosome carrying the Methicillin-resistant gene mecA. The study was aimed to characterize the resistance gene in Methicillin resistant Staphylococcus aureus (MRSA) isolated from skin and wound samples in Kano Metropolis, Northwestern, Nigeria. A total of 235 S. aureus isolates were identified and subjected to MRSA screening. MRSA were phenotypically identified by antibiotic susceptibility testing using agar disc diffusion method. The suspected MRSA were subjected to polymerase chain reaction (PCR). The PCR products were subjected to gel electrophoresis and a DNA ladder were loaded into the gel wells. The gel was examined for the presence of specific amplicons of the expected size for mecA, which is 192bp. Out of a total of 235 isolates of Staphylococcus aureus, only 11 (4.7%) strains were found to be Methicillin resistant Staphylococcus aureus (MRSA). Five out of the 11 isolates show the presence of DNA bands of the expected size for MecA gene which indicated the presence of the resistance genes in the bacterial isolates. It is concluded that MecA gene is one of the gene responsible for methicillin resistance in MRSA.","PeriodicalId":511904,"journal":{"name":"Journal of Biotechnology Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140244390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection 用于检测犬细小病毒感染的三明治酶联免疫吸附试验的开发和标准化
Journal of Biotechnology Research Pub Date : 2024-02-10 DOI: 10.32861/jbr.102.20.25
Karman Kour, Gurpreet Kaur, M. Chandra, D. Narang
{"title":"Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection","authors":"Karman Kour, Gurpreet Kaur, M. Chandra, D. Narang","doi":"10.32861/jbr.102.20.25","DOIUrl":"https://doi.org/10.32861/jbr.102.20.25","url":null,"abstract":"Canine parvovirus (CPV) is a highly contagious viral pathogen affecting dogs aged 6-21 weeks primarily targeting rapidly dividing cells of GI tract, which leads to haemorrhagic gastroenteritis and symptoms such as fever, vomiting, in appetence and abdominal pain. Several variants include CPV-2, CPV-2b and CPV-2c. CPV can be prevented by timely and complete vaccination and can be treated effectively if timely diagnosis is done. It can be diagnosed by effective methods such as PCR, fecal ELISA, HA and HI, the most effective being ELISA and PCR. Therefore, the present research was aimed at developing and standardizing a Double Antibody Sandwich ELISA for the detection of CPV by using rabbit hyperimmune sera as primary antibody and mice hyperimmune sera as secondary antibody.","PeriodicalId":511904,"journal":{"name":"Journal of Biotechnology Research","volume":"11 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139786484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection 用于检测犬细小病毒感染的三明治酶联免疫吸附试验的开发和标准化
Journal of Biotechnology Research Pub Date : 2024-02-10 DOI: 10.32861/jbr.102.20.25
Karman Kour, Gurpreet Kaur, M. Chandra, D. Narang
{"title":"Development and Standardization of Sandwich ELISA for the Detection of Canine Parvovirus Infection","authors":"Karman Kour, Gurpreet Kaur, M. Chandra, D. Narang","doi":"10.32861/jbr.102.20.25","DOIUrl":"https://doi.org/10.32861/jbr.102.20.25","url":null,"abstract":"Canine parvovirus (CPV) is a highly contagious viral pathogen affecting dogs aged 6-21 weeks primarily targeting rapidly dividing cells of GI tract, which leads to haemorrhagic gastroenteritis and symptoms such as fever, vomiting, in appetence and abdominal pain. Several variants include CPV-2, CPV-2b and CPV-2c. CPV can be prevented by timely and complete vaccination and can be treated effectively if timely diagnosis is done. It can be diagnosed by effective methods such as PCR, fecal ELISA, HA and HI, the most effective being ELISA and PCR. Therefore, the present research was aimed at developing and standardizing a Double Antibody Sandwich ELISA for the detection of CPV by using rabbit hyperimmune sera as primary antibody and mice hyperimmune sera as secondary antibody.","PeriodicalId":511904,"journal":{"name":"Journal of Biotechnology Research","volume":"13 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139846314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信
小红书