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LncRSFD Alternative Splicing Modulates the Proliferation and Differentiation of Adipocytes Through Sponging miRNAs LncRSFD选择性剪接通过海绵mirna调节脂肪细胞的增殖和分化

Animal Research and One Health Pub Date : 2025-11-18 DOI: 10.1002/aro2.70040

Jinpeng Wang, Qiuyang Li, Zhiying Wang, Shan Zhang, Kassahun Bekana, Qingfeng Zhang, Juanshan Zheng, Hongwei Xu, Xianyong Lan, Chuanying Pan

{"title":"LncRSFD Alternative Splicing Modulates the Proliferation and Differentiation of Adipocytes Through Sponging miRNAs","authors":"Jinpeng Wang, Qiuyang Li, Zhiying Wang, Shan Zhang, Kassahun Bekana, Qingfeng Zhang, Juanshan Zheng, Hongwei Xu, Xianyong Lan, Chuanying Pan","doi":"10.1002/aro2.70040","DOIUrl":"https://doi.org/10.1002/aro2.70040","url":null,"abstract":"ABSTRACT Adipose deposition critically determines meat quality and metabolic health in livestock, yet its molecular regulation needs further investigation. Recently, long noncoding RNA (lncRNA) alternative splicing events have been found to play a role in adipose tissue accumulation and are abundant in animals. Nonetheless, lncRNA alternative splicing, an underexplored regulatory layer in adipogenesis, may drive functional diversification in fat metabolism. Here, we investigate the functional divergence of two splicing isoforms (lncRSFD‐L and lncRSFD‐S) derived from the lncRNA related to sheep fatty deposits (lncRSFD). Through gain‐of‐function experiments in adipocytes, we demonstrate that lncRSFD‐L/S can significantly promote the proliferation, differentiation, and lipid metabolism of adipocytes. Mechanistically, lncRSFD‐L directly targets three miRNAs (let‐7b, miR‐3959‐3p, and miR‐323a‐5p), while lncRSFD‐S specifically binds to let‐7b and miR‐323a‐5p. It is worth noting that these miRNAs display dual regulatory effects: they suppress proliferation and viability of adipocytes but simultaneously promote differentiation and lipid metabolism. This research advances our understanding of alternative splicing mechanisms, and provides valuable insights for molecular breeding strategies.","PeriodicalId":511814,"journal":{"name":"Animal Research and One Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/aro2.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147885689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ursolic Acid Alleviates Orchitis via Modulating Ferroptosis in Peritubular Myoid Cells of Testis 熊果酸通过调节睾丸小管周围肌样细胞的铁下垂减轻睾丸炎

Animal Research and One Health Pub Date : 2025-11-12 DOI: 10.1002/aro2.70041

Yang Wang, Tianqi Jin, Zexin Chen, Ye Gong, Qimin Liu, Zifang Wu, Heran Cao, Yan Li, Yuyan Liu, Tianhao Yang, Zhi‐Hong Jiang, Wuzi Dong

{"title":"Ursolic Acid Alleviates Orchitis via Modulating Ferroptosis in Peritubular Myoid Cells of Testis","authors":"Yang Wang, Tianqi Jin, Zexin Chen, Ye Gong, Qimin Liu, Zifang Wu, Heran Cao, Yan Li, Yuyan Liu, Tianhao Yang, Zhi‐Hong Jiang, Wuzi Dong","doi":"10.1002/aro2.70041","DOIUrl":"https://doi.org/10.1002/aro2.70041","url":null,"abstract":"ABSTRACT Chronic stress adversely affects reproductive health, increasing susceptibility to pathologies such as orchitis. Additionally, the maintenance of iron homeostasis is essential for preserving the function of the testicles, and iron overload leads to lipid peroxidation and iron accumulation, which induces testicular cell ferroptosis. Ursolic acid (UA), a pentacyclic triterpenoid compound naturally occurring in various plants, exhibits therapeutic potential as an agent for inflammatory and chronic degenerative diseases. However, its involvement in orchitis and ferroptosis remains unclear. The aim of this research was to elucidate the impact of UA on ferroptosis in testicular inflammation. The histopathological data revealed inflammatory infiltration in the testicular tissues from accidently deceased adult forest musk deer (FMD) under chronic stress. In vitro, UA effectively attenuated tumor necrosis factor‐α (TNF‐α)‐induced inflammation. Additionally, UA suppressed ferroptosis through the regulation of the breakdown of excessive lipid peroxides, enhancement in the activity of antioxidant enzymes, and activation of intracellular iron metabolism. Taken together, the current study elucidates multi‐target and multi‐pathway therapeutic effects of UA on reproductive homeostasis. These findings highlight that UA may serve as a potential functional component in the prevention of chronic stress‐induced diseases.","PeriodicalId":511814,"journal":{"name":"Animal Research and One Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/aro2.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0