Journal of Advanced Biomedical Sciences最新文献

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Cytotoxicity of Ethanolic and Methanolic Extracts of Medicago sativa L. (Alfalfa) on K562 Myeloid Cancer Cell Lines 紫花苜蓿乙醇和甲醇提取物对K562骨髓癌细胞的细胞毒性研究

Journal of Advanced Biomedical Sciences Pub Date : 2024-08-26 DOI: 10.18502/jabs.v14i3.16360

Mahdieh Raeeszadeh, Afsoon Afshari, Samaneh Kazemiafshar, Hajar Kazemiafshar, Ali Akbar Amiri

{"title":"Cytotoxicity of Ethanolic and Methanolic Extracts of Medicago sativa L. (Alfalfa) on K562 Myeloid Cancer Cell Lines","authors":"Mahdieh Raeeszadeh, Afsoon Afshari, Samaneh Kazemiafshar, Hajar Kazemiafshar, Ali Akbar Amiri","doi":"10.18502/jabs.v14i3.16360","DOIUrl":"https://doi.org/10.18502/jabs.v14i3.16360","url":null,"abstract":"Background & Objectives: For centuries, the alfalfa (Medicago sativa L.) plant has been recognized for its versatile and active role in treating various diseases. Not only has it been utilized as a therapeutic agent, but it has also been served as a dietary component for both animals and humans. Given the distinctive attributes of this plant in ethnopharmacology, this study aimed to investigate the effects of ethanolic and methanolic extracts of M. sativa L. on the K562 myeloid cell line under in vitro conditions. Materials & Methods: The phytochemical composition of M. sativa L. was determined through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Cell viability was assessed using the MTT assay, wherein K562 cells were subjected to varying concentrations (50–100 μg/mL) of methanolic and ethanolic extracts over 24, 48, and 72- hour intervals to determine the IC50. Subsequently, the most promising IC50 result was employed in flow cytometry (Flow Jo Software) analysis. Results: Active constituents identified included phytol, phenol, linolenic acid, and glycine. Statistical analysis revealed a time-dependent but not dose-dependent effect. It was noteworthy that the IC50 for the methanolic extract after 72 hours was 9.45 μg/mL, whereas it was 19.3 μg/mL for the ethanolic extract. Flow cytometry analysis indicated that the methanolic extract caused 49.16% and the ethanolic extract caused 15.42% of cell death. Conclusion: The results demonstrated that the ethanolic extract of alfalfa is more effective than the methanolic extract on the K562 cell line. Therefore, M. sativa L. potential application in myeloid cancer therapy can be investigated in more details","PeriodicalId":511515,"journal":{"name":"Journal of Advanced Biomedical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://publish.kne-publishing.com/index.php/JABS/article/download/16360/15673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Lavender Aqueous Extract on the Levels of Oxidative Stress Markers in the Sera and Tissues from Male Sprague–Dawley Rats with Chronic Mild Stress Induced Depression 薰衣草水提物对慢性轻度应激性抑郁症雄性Sprague-Dawley大鼠血清和组织中氧化应激标志物水平的影响

Journal of Advanced Biomedical Sciences Pub Date : 2023-10-17 DOI: 10.18502/jabs.v13i4.13898

Aghazadeh Ghadim Mir Behrad, Mahmoodzadeh Amir, Neisy Asma, Khoshde Zahra

{"title":"Effects of Lavender Aqueous Extract on the Levels of Oxidative Stress Markers in the Sera and Tissues from Male Sprague–Dawley Rats with Chronic Mild Stress Induced Depression","authors":"Aghazadeh Ghadim Mir Behrad, Mahmoodzadeh Amir, Neisy Asma, Khoshde Zahra","doi":"10.18502/jabs.v13i4.13898","DOIUrl":"https://doi.org/10.18502/jabs.v13i4.13898","url":null,"abstract":"Background & Objective: Oxidative stress is associated with the pathology of various neuropsychiatric disorders, such as depression and anxiety. The aim of the study was to examine the impacts of the lavender aqueous extract (LAE) on the levels of oxidative stress markers in an animal model with chronic mild stress induced depression. Materials & Methods: 36 adult male Sprague–Dawley rats were haphazardly split into 6 groups (n=6). Rats in the test groups were put through five weeks of chronic mild stress. The sucrose preference test (SPT) confirmed depressive-like behaviors in the rats. Rats in both unstressed and stressed control groups received LAE (200 or 400 mg / kg) and distilled water respectively. The specific activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (Gpx), malondialdehyde (MDA) and as well as the total content of glutathione (GSH) were examined in the serum, liver and prefrontal cortex (PFC) using colorimetric assays. Results: The stress group experienced a significantly decreased level of GSH, SOD, GR, Gpx and CAT, and a statistically considerable augment in MDA level in in the serum, PFC and liver p<0.05. LAE at both doses significantly reversed the depression-caused oxidative stress markers in all the studied tissues. No significant difference was observed between the two used dosages of LAE. Conclusion: LAE is able to ameliorate depression-induced pathology possibly through anti-oxidant activity.","PeriodicalId":511515,"journal":{"name":"Journal of Advanced Biomedical Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://publish.kne-publishing.com/index.php/JABS/article/download/13898/13831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147905933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2