Cryoletters最新文献

{"title":"Role of vitamin E and selenium in enhancing semen freezability and fertility in subfertile bucks","authors":"Chetna Gangwar, M Arivazhagan, B. Rai, N. Ramachandran, Shriprakash Singh, Anshuman Kumar, Anil Kumar Mishra, R. Pourouchottamane, Pradeep Kumar, Omprakash Jajriya","doi":"10.54680/fr25610110712","DOIUrl":"https://doi.org/10.54680/fr25610110712","url":null,"abstract":"BACKGROUND: In the quest for sustainable nutritional security, small ruminants play a crucial role, yet many producers face a common stumbling block: insufficient fertile bucks in their herds. This often results in disappointing production outcomes. A simple yet powerful solution lies in the supplementation with minerals and vitamins, thereby enhancing fertility and ensuring that small ruminant herds thrive and contribute to a more secure and productive agriculture. OBJECTIVE: To determine the effects of vitamin E and Se on semen quality and freezability, antioxidant activity, testosterone level, and fertility of Jakhrana bucks. MATERIALS AND METHODS: Eighteen Jakhrana bucks were classified as subfertile bucks based on their semen characters and fertility test. The bucks were randomly allocated into three equal groups and subjected to the following treatments: G1, control; G2, injected with Vit E 250IU/head + Se 0.1 mg/kg body weight.; G3, injected with Vit E 500 IU/head + Se 0.2 mg/kg body weight once weekly for 8 weeks. Semen and blood samples were collected twice weekly for all bucks to assess semen quality and semen was frozen and the post-thaw semen quality parameters (such as progressive motility, viability, abnormality and acrosomal integrity) were evaluated. Serum testosterone level and antioxidant capacity were also measured. At the 8th week of the study, 60 multiparous estrus does (20 does for each group) were exposed for mating to assess the fertility of buck after treatment. RESULTS: Fresh and post-thaw semen quality parameters were significantly (p <0.05) higher in G3 in comparison with G2 and G1, respectively. Also, testosterone level was significantly (p <0.05) increased at the 3rd week (W3) in the G2 and G3 groups in comparison with control group. Fertility and kidding rates were significantly (p <0.05) higher in does who were mated with bucks of G3. CONCLUSION: Injection of vitamin E and selenium improved the semen quality parameters and most importantly the fertility of bucks.","PeriodicalId":511263,"journal":{"name":"Cryoletters","volume":"46 6","pages":"382-392"},"PeriodicalIF":0.0,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147919593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viscoelastic properties of decellularized and freeze-dried human dermis between −90°C and 40°C","authors":"Q. Jia, Senli Huang, Tang, Wendell Q. Sun","doi":"10.54680/fr24110110612","DOIUrl":"https://doi.org/10.54680/fr24110110612","url":null,"abstract":"BACKGROUND: Human donor skin is processed to make the acellular dermis matrix (ADM) for tissue repair and regeneration. There is no data on the viscoelastic properties of ADM at room and subzero temperatures. OBJECTIVE: The work evaluated the temperature dependence of viscoelastic properties\u0000 of freeze-dried ADM. MATERIALS AND METHODS: Donor skin was de-epidermized, de-cellularized and freeze-dried with trehalose as the lyo-protectant. Glass transition of freeze-dried ADM was measured by differential scanning calorimeter (DSC), and viscoelastic properties were examined by dynamic\u0000 mechanical analyzer (DMA). RESULTS: At the low moisture range (1.4 ± 0.5%), the glass transition temperature (Tg) of freeze-dried ADM was 90°C to 100°C. As the moisture content increased, the Tg decreased steadily. At the high moisture range (10.8 ± 2.9%),\u0000 the Tg was 40°C to 60°C. There were large donor-to-donor variations in viscoelastic properties of freeze-dried ADM as demonstrated by the changes in storage modulus (G’), loss modulus (G”) and damping factor tan δ(G”/G’). However, the trends of the\u0000 temperature dependence for G’, G” and tan δ were similar among all 8 donors. For each donor, changes in G’ and G” were relatively small between −90°C and 40°C, and G’ was at least one order of magnitude greater than G”. Two viscoelastic\u0000 relaxations were observed in freeze-dried ADM, one at −20°C and the other at −60°C respectively. CONCLUSION: Freeze-dried ADM was protected in the glassy carbohydrate matrix. DMA observed two viscoelastic relaxations (i.e., α process at −20°C and β\u0000 process at −60°C). Overall changes in G’ and G” of freeze-dried ADM were relatively small within one order of magnitude between −90°C and 40°C.","PeriodicalId":511263,"journal":{"name":"Cryoletters","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140524903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}