The Ukrainian Biochemical Journal最新文献

{"title":"Iodide n,π-chelate complexes of platinum(II) based on N-allyl substituted thioureas and their effect on the activity of hepatobiliary system enzymes in comparison with chloride analogs","authors":"V. Orysyk, L. Garmanchuk, S. Orysyk, Yu. L. Zborovskii, S. Shishkina, I. Stupak, P. Novikova, D. Ostapchenko, N. Khranovska, V. Pekhnyo, M. Vovk","doi":"10.15407/ubj96.03.075","DOIUrl":"https://doi.org/10.15407/ubj96.03.075","url":null,"abstract":"The search for new effective drugs in the treatment of neoplasm remains relevant even today, since the adaptation of transformed cells to the action of classical drugs contributes to the emergence of drug resistance­. This applies to a number of classic chemotherapy drugs of the platinum series, in particular cisplatin. In this work, we describe the effect of novel analogs of cisplatin on HepG2 cells and on the key enzyme of antioxidant protection system gammaglutamyltranspeptidase, which plays an important role in the acquisition of drug resistance to anticancer drugs by tumor cells. New mononuclear iodide n,π-chelate complexes of Pt(II) with substituted thioureas N-allylmorpholine-4-carbothioamide or 3-allyl-1,1-diethylthiourea were obtained as analogs of cisplatin. All compounds were investigated by UV-Vis, IR, and 1H/13С NMR spectra. Complex I was described by single-crystal X-ray diffraction study. Also, the effect of these analogs on alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, which are marker enzymes of liver cells, release of which into the blood indicates liver pathologies, was investigated. All studies were carried out in comparison with chloride n,π-chelate complexes of platinum obtained earlier (however, the effect of these chloride analogs of platinum on enzymes of the hepatobiliary system was investigated for the first time in this work). The results have shown that the studied compounds are better cytostatics/cytotoxics than cisplatin both according to IC50 and apoptosis level of HepG2 cells. It is established that, for the most part, effect of the studied complexes is reduced to a decrease in the degree of malignancy of cells of hepatocyte lines and the activity of LDH and GHT, as well as a decrease in consumed glucose. Keywords: alanine aminotransferase, aspartate aminotransferase, gammaglutamyltranspeptidase, lactate dehydrogenase, NMR spectroscopy, n‚π-chelates, thioureas","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"74 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alpha-L-fucosidase as a putative prognostic biomarker in breast cancer","authors":"Z. M. A. A. Hamodat, H. H. Abdulwahhab, A. R. M. T. Hamodat","doi":"10.15407/ubj96.03.057","DOIUrl":"https://doi.org/10.15407/ubj96.03.057","url":null,"abstract":"Search for reliable biomarkers for predicting progression of breast cancer is essential in managing the disease. So, we are trying to provide new insights into the potential role of alpha-fucosidase (AFU) as a putative prognostic biomarker in breast cancer as compared to classic markers. The study included 56 women with breast cancer; 25 had early breast cancer, and the rest (31) had metastatic breast cancer. Thirty healthy women were considered a control group. Early breast cancer patients had a significantly increased (P ≤ 0.0001) AFU level compared with the control group. A non-significant difference in the De-ritis ratio appeared for early breast cancer compared with control. Metastatic breast cancer had a significantly (P ≤ 0.0001) increased AFU and De-ritis ratio compared with early breast cancer and the control group. A positive significant (P = 0.01) correlation exists between AFU level, age factor (r = 0.295), and the De-ritis ratio in breast cancer patients. We can conclude that it is possible to consider alpha-L-fucosidase (AFU) as a putative prognostic biomarker in breast cancer more potent than the ratio of De-Ritis. Moreover, the coincidence of elevated AFU and De-ritis levels in metastatic breast cancer gives us an idea of the stage of the disease. Keywords: alpha-L-fucosidase, early breast cancer, metastasis, the De-ritis ratio","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"1 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141341854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of The Ukrainian Biochemical Journal thanks to Collaboration with RECOOP HST Association","authors":"M. Grigorieva, V. Chernyshenko, S. Vari","doi":"10.15407/ubj96.03.143","DOIUrl":"https://doi.org/10.15407/ubj96.03.143","url":null,"abstract":"The article examines the impact of cooperation between The Ukrainian Biochemical Journal (UBJ) and the international association RECOOP HST on the journal’s development in 2019-2023. During this period, 324 articles were published in the journal. Most of them are written by Ukrainian authors (79.9%). Foreign authors (20.1%) are representatives of 15 countries. An online manuscript handling and reviewing system were implemented on the journal’s website, which increased the number of articles received by the editorial office and allowed for a more careful selection of manuscripts at the initial evaluation stage. As a result, the percentage of rejected manuscripts increased from 36.5 to 67.7%. RECOOP’s support has also played a key role in improving the quality of UBJ’s publications through the renewal of its editorial board and expanding its international reviewer base. In collaboration with RECOOP, eight special issues of UBJ were produced separately, including four themed issues. In total, 56 articles (110 citations) were published in these issues. The number of visitors to UBJ’s website almost tripled during the mentioned period. Currently, UBJ’s Scimago Scientific Journal Rank (SJR) for 2022/2023 is 0.169, the h-index is 20, and the Impact Score is 0.63. A conclusion is made that the cooperation with RECOOP contributed to the publication of better scientific articles, the involvement of highly qualified experts in the peer review process, and stricter adherence by the journal to the fundamental provisions and ethical standards, thereby leading to an elevation in UBJ’s rating and recognition in the global scientific arena. Keywords: COMET, increasing UBJ’s global visibility, project implementation, RECOOP, thematic issue, UBJ","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"22 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141343018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic regularities and a possible mechanism of ATP non-enzymatic hydrolysis induced by calix[4]arene С-107","authors":"S. O. Kosterin, Т. О. Veklich, O. І. Kalchenko, A. I. Vovk, R. Rodik, О. А. Shkrabak","doi":"10.15407/ubj96.03.108","DOIUrl":"https://doi.org/10.15407/ubj96.03.108","url":null,"abstract":"The kinetic model of calix[4]arene-induced ATP hydrolysis was elaborated. It is assumed that calix[4]­arene С-107 molecules form a complex with nucleoside triphosphate, ensuring the release of inorganic phosphate Рі, and then switch into an inactive state. Inactive calix[4]arene molecules are no longer able to form a complex with ATP and, accordingly, to provide hydrolysis of nucleoside triphosphate. In the author’s experimental studies, it was possible to explain the kinetic properties of the reaction, namely: the insignificant output of the reaction end product Pi; the quantitative regularities of the plateau (time-wise) accumulation of the reaction product when the concentration of calix[4]arene C-107 or ATP changes; the reciprocal dependence of the ATP half conversion on its concentration; the correspondence of the initial reaction rate dependence on the calix[4]arene and ATP concentration to the Michaelis-Menten equation. The final decision regarding the molecular mechanism of calix[4]arene-induced ATP hydrolysis requires further experimental and theoretical studies. Keywords: calixarenes, empirical kinetic analysis, hydrolysis of ATP","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"73 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenformin attenuates the oxidative-nitrosative stress in the liver of rats under long-term ethanol administration","authors":"A. Mykytenko, O. Akimov, G. Yeroshenko, K. Neporada","doi":"10.15407/ubj96.03.022","DOIUrl":"https://doi.org/10.15407/ubj96.03.022","url":null,"abstract":"Modulation of the AMP-activated protein kinase (AMPK) pathway activity is considered to be a promi­sing option in the development of approaches to chronic alcoholic hepatitis treatment. Phenformin, which is a biguanide, has been reported to increase AMPK activity. The aim of this work was to estimate the effect of phenformin as AMPK activator on the development of oxidative-nitrosative stress in the liver of rats under conditions of long-term ethanol administration. The experiments were performed on 24 male Wistar rats, divided into 4 groups: control; animals, which received phenformin hydrochloride orally at a dose of 10 mg/kg daily for 63 days; animals with a forced intermittent alcoholization for 5 days by intraperitoneal administration of 16.5% ethanol solution in 5% glucose at the rate of 4 ml/kg b.w. and subsequent transfer to 10% ethanol as the only source of drinking; animals with chronic alcohol hepatitis simulation and phenformin administration. Superoxide dismutase, catalase, NO synthase isoforms activity, superoxide anion radical production, concentration of malonic dialdehyde, peroxynitrite, nitrites, nitrosothiols concentration and oxidative modification of proteins (OMP) were estimated in liver homogenates. The increased production of oxygen and nitrogen active forms and OMP intensification in the liver of rats under long-term administration of ethanol was detected. Phenformin introduction under long-term ethanol administration was shown to limit the excess peroxynitrite formation and to prevent oxidative damage to rat liver proteins. Keywords: AMP-activated protein kinase., chronic alcoholic hepatitis, liver, oxidative and nitrosative stress, phenformin","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"58 51","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141338883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embelin mitigates hepatotoxicity induced by isoniazid and rifampicin in rats","authors":"O. F. Mosa","doi":"10.15407/ubj96.03.048","DOIUrl":"https://doi.org/10.15407/ubj96.03.048","url":null,"abstract":"Isoniazid and rifampicin are reliable drugs against tuberculosis, but while effective, their use is associated with the risk of drug-induced liver damage. Embelin, a natural parabenzoquinone derived from the Embelia ribes plant, has gained attention for its potential therapeutic properties, antioxidant and organ-protective effects. The study aimed to assess the hepatoprotective properties of embelin against liver dama­ge induced by isoniazid and rifampicin in rats. Wistar rats were used, and liver damage was induced by administration of isoniazid (100 mg/kg) and rifampicin (100 mg/kg). Embelin was given at doses of 50, 75, and 100 mg/kg for 21 days. All the drugs were given orally. Serum levels of the oxidative stress markers, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) activity measured by enzymatic assay kits (Elabscience, China), and the levels of tumour necrosis factor-α (TNF-α), interleukins IL-1β and IL-6 measured by ELISA kits (Randox, UK) were estimated. Embelin administration at varying doses effectively restored AST, ALT, ALP, SOD and catalase activity and notably decreased MDA and nitric oxide concentration as well as expression of inflammatory cytokines TNF-α, IL-1β and IL-6 in the serum of animals with drug-induced liver damage. These findings underscore embelin’s hepatoprotective effects, likely attributed to its radical scavenging properties and ability to suppress cytokine production. Keywords: antioxidant effect, cytokine suppression, embelin, hepatoprotection, isoniazid, rifampicin","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"21 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141342034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional chitosan-based hydrogels: characterization and evaluation of biocompatibility and biodegradability in vitro","authors":"N. Manko, M. Lootsik, V. Antonyuk, I. Ivasechko, N. Skorokhyd, H. Kosiakova, O. Mehed’, T. Horid’ko, N. Hula, O. Klyuchivska, R. Panchuk, N. Pokhodylo, О. Barabash, T. Dumych, R. Stoika","doi":"10.15407/ubj96.01.080","DOIUrl":"https://doi.org/10.15407/ubj96.01.080","url":null,"abstract":"Creation of novel remedies efficient in supporting wound healing remains an actual task in pharmacology. Hydrogels showed high efficiency in wound healing and tissue regeneration due to viscosity, elasticity and fluidity that provide them with functional characteristics similar to that in extracellular matrix. The aim of the study was to create chitosan-based hydrogels functionalized with different components (chondroitin-6-sulfate, hyaluronic acid, N-stearoylethanolamine) and to estimate their biocompatibility and biodegradabili­ty in vitro. For the first time, a lipid substance N-stearoylethanolamine (NSE) known as suppressor of pro-inflammatory cytokines expression was used as hydrogel component (1.95 mg/g). FTIR analysis confirmed the complexation of chitosan molecule with hyaluronate, chondroitin-6-sulfate, NSE. MTT-test and Trypan blue exclusion test were used to study hydrogels cytotoxicity towards human cells of different tissue origin. Biodegradability of hydrogels was evaluated using direct hydrogel contact with cells and cell-independent degradation. It was shown that chondroitin-6-sulfate (<2 mg/ml), hyaluronic acid (<2 mg/ml) and NSE (26 µg/ml) did not demonstrate significant toxic effects towards pseudonormal human cells of the MCF10A, HaCat, HEK293 lines and mouse cells of the Balb/3T3 line. The studied hydrogels were stable in saline solution, while in a complete culture medium containing 10% fetal bovine blood serum they underwent degradation in >24 h. The identified biodegradability of the chitosan-based hydrogels is important for the release of noncovalently immobilized NSE into biological medium. Further studies on laboratory animals with experimental wounds are expected to explore the potential of created hydrogels as anti-inflammatory and wound-healing agents. Keywords: biodegradability, chitosan hydrogels, chondroitin-6-sulfate, FTIR analysis, human pseudonormal cells, hyaluronic acid, N-stearoylethanolamine, toxicity","PeriodicalId":508485,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"38 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139957405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}