Journal of the Professional Association for Cactus Development最新文献

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Optimization of DNA Extraction for ITS/U4U3 analysis of Rhipsalis baccifera 用于 ITS/U4U3 分析的红腹角雉 DNA 提取方法的优化

Journal of the Professional Association for Cactus Development Pub Date : 2024-07-03 DOI: 10.56890/jpacd.v26i.563

Tomás Rivas-García, David Córdova-Pérez, Roberto Carlos Arredondo-Espinoza, Bernardo Murillo-Amador, R. González-Estrada, J. Reyes-Pérez, J. Torres-Rodríguez, Rubí A. Martínez-Camacho, J. Alejandre-Rosas

{"title":"Optimization of DNA Extraction for ITS/U4U3 analysis of Rhipsalis baccifera","authors":"Tomás Rivas-García, David Córdova-Pérez, Roberto Carlos Arredondo-Espinoza, Bernardo Murillo-Amador, R. González-Estrada, J. Reyes-Pérez, J. Torres-Rodríguez, Rubí A. Martínez-Camacho, J. Alejandre-Rosas","doi":"10.56890/jpacd.v26i.563","DOIUrl":"https://doi.org/10.56890/jpacd.v26i.563","url":null,"abstract":"Genetic analysis of plants relies on high yields of pure DNA. For Rhipsalis baccifera this represents a great challenge since its plant tissue can accumulate large amounts of mucilage, polysaccharides, polyphenols and secondary metabolites, which co-purify with amplifiable DNA. These contaminating compounds lead to a poor yield and prevent acces to PCR-based analysis. These contaminating compounds lead to a poor DNA yield and prevent access to PCR-based analysis. A number of factors, including choice of plant tissue, tissue preparation, and modifications of the extraction buffer, can impact on DNA extraction process. In this study, four different DNA extraction procedures were tested aiming to develop a simple protocol based on CTAB buffer. The obtained results showed that the use of the outer cuticle of old lyophilized tissue allowed reliable results in R. baccifera plants with a good purity ranging from 1.6 to 1.8 and high DNA yield yield ? 500 ng ?L-1.","PeriodicalId":505067,"journal":{"name":"Journal of the Professional Association for Cactus Development","volume":" 28","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141680756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agronanotechnology in the arid zones of northern Mexico: Research, challenges, and new trends 墨西哥北部干旱地区的农用技术：研究、挑战和新趋势

Journal of the Professional Association for Cactus Development Pub Date : 2024-01-19 DOI: 10.56890/jpacd.v26i.534

Mónica Anzaldo-Montoya, Yoscelina I. Hernandez-Garcia, Luis Guillermo Hernández-Montiel, Luis Hernández Adame

{"title":"Agronanotechnology in the arid zones of northern Mexico: Research, challenges, and new trends","authors":"Mónica Anzaldo-Montoya, Yoscelina I. Hernandez-Garcia, Luis Guillermo Hernández-Montiel, Luis Hernández Adame","doi":"10.56890/jpacd.v26i.534","DOIUrl":"https://doi.org/10.56890/jpacd.v26i.534","url":null,"abstract":"The agri-food systems in arid zones face challenges related to climate change, desertification, and soil contamination from agrochemicals' actions, among others. The agronanotechnology can generate alternatives to these problems, but it is necessary to monitor its development to have elements to apply policies that are oriented to the attention of these regions with a social perspective. This scientific paper aims to present provide a study of the situation of agronanotechnology in the arid zones of northern Mexico. Using bibliometric tools, the academic output in the field and map the public research agenda, focusing on providing the set of crops to which the local research is directed was analyzed. Furthermore, the literature associated with the risks to human health and the environment derived from some nanomaterials of agri-food interest and clarify the areas of opportunity and trends was reviewed. The results show a total of 224 research articles authored by 1029 scholars in 161 journals published from 2004 to 2022; that research on this topic reached its highest value in 2018 and then levels off at a value around of ~37%. The findings show a research agenda related to crops of economic interest for the export market like tequila, tomato, avocado, chili, watermelon. In México, agronanotechnology is an emerging field that deals with some crops of economic interest, but its development needs to be intertwined with the needs of rural producers.","PeriodicalId":505067,"journal":{"name":"Journal of the Professional Association for Cactus Development","volume":"15 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139525486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0