Discussion of Clinical Cases最新文献

{"title":"Exploration on the protective effect of liraglutide on renal injury in diabetic nephropathy rats based on ROS-NLRP3 inflammasomes","authors":"Jun Xue, Xiuqi Ma, Xuehui Wu, Jiali He, Weiling Song, Hongju Li","doi":"10.5430/dcc.v10n3p18","DOIUrl":"https://doi.org/10.5430/dcc.v10n3p18","url":null,"abstract":"Objective: To investigate the effect of liraglutide on diabetic nephropathy in rats and its regulatory mechanism. Methods: The diabetic nephropathy rat model was constructed with high-glucose-high-fat diet in combination with STZ, and was randomly divided into normal saline group and liraglutide group. The rats in liraglutide group were given sc 200 μg/kg of liraglutide, and the rats in normal saline group were given sc 20 mg/kg of normal saline twice a day for 4 weeks. The normal control group was not treated with any treatment. The biochemical indexes such as rat body weight, 24-hour urine total protein quantification (UTP), fasting blood glucose (FPG), triglyceride (TG), cholesterol (TC), blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. HE staining, Masson staining and PAS staining were used to observe the pathologically morphological changes in renal tissues. Western-blot was used to detect the expression of NLRP3 inflammasome-related protein in renal tissues. Elisa was used to measure the serum levels of interleukin-18 (IL-18) and IL-1β. SPSS 26.0 statistical software and Graph Prism 9.0 software were used for analysis and mapping. The t-test was used for the comparison of measurement data between two groups, one-way ANOVA was used for the comparison among multiple groups, and Tukey’s test was used for the comparison in the same group. Results: Compared with the normal saline group, FBG, UTP, BUN, Scr, TC and TG in the liraglutide group were significantly decreased (p < .01), the glomerular basement membrane was slightly thickened, with the tubular lumen slightly dilated and the lesion damage alleviated; the expression levels of NLRP3, ASC and caspase-1 inflammasome-related proteins were decreased (p < .01), and the levels of IL-18 and IL-1β were decreased (p < .01).Conclusion: Liraglutide can inhibit the activation of NLRP3 inflammasome mediated by oxidative stress in renal tissues through ROS-NLRP3 inflammasome pathway, thereby inhibiting the inflammation, and finally playing an anti-diabetic nephropathy renal injury role.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"8 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141640179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress of mesenchymal stem cell exosomes in multiple organ inflammatory diseases","authors":"Biao Zhou, T. Ba, Ling-feng Wang, Yangyang Li","doi":"10.5430/dcc.v10n3p12","DOIUrl":"https://doi.org/10.5430/dcc.v10n3p12","url":null,"abstract":"Inflammation is generally considered as a pathological process, and the occurrence of multi-organ inflammatory diseases is usually accompanied by an inflammatory response. Persistent inflammation can eventually lead to organ dysfunction. In recent years, the study of mesenchymal stem cell-derived exosomes has found its unique anti-inflammatory effect, which provides a new idea for the treatment of multi-organ inflammatory diseases. This article reviews the effect of mesenchymal stem cell exosomes on multi-organ inflammatory diseases.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"41 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141345360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovation and practice in the service model of the nursing team in the 37°C comprehensive ward","authors":"Ruijuan Huang, Yuxin Zhang, Guanfeng Fu","doi":"10.5430/dcc.v10n3p6","DOIUrl":"https://doi.org/10.5430/dcc.v10n3p6","url":null,"abstract":"Objective: To establish a nursing service model of 37°C “six-center” services and study the effect of this model on improving nursing services in the course of nursing work.Methods: The semi-structured interview method and group meeting method are used to establish the 37°C nursing service model and implement the “general health” concept of “benevolence-based and central enterprise quality.” The newly established 37°C “six-center service” (enthusiastic reception, patient explanation, careful observation, sincere help, warm-hearted delivery and heart-to-heart visit) nursing model in the comprehensive ward of Baogang Hospital (Inner Mongolia) runs through the three phases of patient admission, hospitalization and discharge, providing patients with warm care services, so that patients can truly enjoy the warmth of 37°C “six-center service” nursing and friendly family flair during the peri-hospitalization period.Results: (1) The established 37°C “six-heart service” nursing service model has been treated as a nursing service path and is widely used in the hospital; (2) After the implementation of the 37°C nursing service, patients, nurses, doctors, hospitals and the society show an effectively improved satisfaction; (3) The 37°C “six-heart service” nursing service model is established as a hospital nursing service brand; (4) The 37°C “six-heart service” nursing service brand has received enthusiastic responses in Baotou, Health Management Technology Co., Ltd. and GENERTEC.Conclusions: The established 37°C “six-heart service” nursing service model can effectively improve the quality of nursing service and establish a nursing service brand, which can be promoted across the country.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"90 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141004343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis and treatment of neonatal machete syndrome","authors":"Huimin Li","doi":"10.5430/dcc.v10n2p21","DOIUrl":"https://doi.org/10.5430/dcc.v10n2p21","url":null,"abstract":"Aim: To provide suggestions for clinical diagnosis, treatment and eugenics through the retrospective analysis of a case of neonatal machete syndrome.Methods: The clinical data of a newborn with machete syndrome admitted to the pediatrics department of Baogang Hospital of Inner Mongolia were analyzed, and suggestions of eugenics were provided based on the literatures.Results: The child, male, natural conception, G2P2, gestational age 38+4 weeks, was born by cesarean section (at am 09:24 on November 13th, 2021). He was hospitalized in our department for 9 days and given NCPAP positive pressure ventilation to give oxygen and anti-infection symptomatic treatment. The dyspnea of the child was not significantly alleviated, and the heart murmurs became increasingly obvious. The child was transferred to the neonatology Department of the Seventh Medical Center of the PLA General Hospital for further treatment on 9 days after birth. After admission, piperacillin and tazobactam were treated with anti-infection, high-frequency oscillation mode of invasive ventilator, limited fluid volume and other symptomatic treatments. Fiberbronchoscopy showed atelectasis of right lung and tracheomalacia of left lung. Echocardiography showed partial anomalous pulmonary venous drainage, atrial septal defect (secondary foramina), inferior vena cava dilatation, pulmonary hypertension, initial coronary artery dilatation, and patent ductus arteriosus. CT 3D imaging of pulmonary veins showed: Congenital heart disease: partial anomalous pulmonary venous drainage (subcardial), consideration of \"machete syndrome,\" atrial septal defect, consideration of pulmonary hypertension, right pulmonary artery stenosis, right lower lung receiving abdominal aorta blood supply, right lung tissue dysplasia, and widening of inferior vena cava pulmonary veins. Therefore, the comprehensive diagnosis was \"machete syndrome.\" Considering the high surgical risk of the child, conservative symptomatic treatment was recommended, and the family requested discharge after 22 days of treatment. The patient died at home at 3 months follow-up after discharge.Conclusions: Machete syndrome is a rare congenital property of abnormal partial or complete pulmonary venous drainage from the right or left lung to the inferior vena cava. With an incidence of approximately 2 in 100,000 live births, the syndrome is commonly associated with right lung dysplasia, pulmonary sequestration, persistent left superior vena cava, and right tilt of the heart. In this case, the child had these syndromes after birth, since the primary hospital did not recognize the disease, the child was clearly diagnosed in the higher hospital. Children with machete syndrome can be treated with surgery in the early neonatal period, but the indication of surgical treatment is still controversial, and the need for surgery is evaluated according to the severity of the patient's symptoms and pulmonary circulation blood flow.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"128 34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139605256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and prognostic analysis of AEG-1, NKD-1and β-catenin in gastric cancer tissues","authors":"Ruizhen Wang, Weidong Guo","doi":"10.5430/dcc.v9n4p29","DOIUrl":"https://doi.org/10.5430/dcc.v9n4p29","url":null,"abstract":"Objective: To investigate the expressions of AEG-1, NKD-1 and β-catenin in gastric cancer (GC), and analyze their relationship with clinicopathological features and prognosis and the correlation among the three proteins.Methods: Fifty GC tissues and 50 adjacent normal tissues of patients who underwent radical gastrectomy in the Third Affiliated Hospital of Inner Mongolia Medical University during December 2015 to June 2017 were collected. The expressions of AEG-1, NKD-1 and β-catenin were detected by immunohistochemical SP method and qRT-PCR. The relationship between positive expression and clinical features and survival time of patients was analyzed, and the correlation between the three was analyzed.Results: The positive expression rates of AEG-1 and β-catenin in gastric cancer were 74% (37/50), 60% (30/50), respectively, which was significantly higher than that of paracancer tissue (18% [9/50] and 26% [13/50]). The positive expression rate of NKD1 in gastric cancer tissues was 20% (10/50), which was significantly lower than that in adjacent tissues [88% (44/50)]; and the differences were statistically significant (p < . 05). AEG-1 mRNA expression level (1.0148±0.0019) and β-catenin mRNA expression level (1.0000±0.0005) in gastric cancer tissues were significantly higher than that of AEG-1 mRNA expression level (1.0002±0.0036) and β-catenin expression level in adjacent tissues mRMA expression level (0.9984±0.0012), and the differences were statistically significant (p < .05). The expression level of NKD1 mRNA in gastric cancer tissues (1.0008±0.0073) was significantly lower than that in adjacent tissues (1.0291±0.0041), and the differences were statistically significant (p < .05). The positive expression of AEG-1, NKD1 and β-catenin in gastric cancer tissues was not related to age, sex or tumor size (p > .05), but was related to differentiation degree, TNM stage and lymph node metastasis (p < .05). In gastric cancer, there was a positive correlation between AEG-1 and β-catenin protein expression (r = .678, p < .001), and a negative correlation between AEG-1 and NKD1 protein expression (r = -.323, p = .012). β-catenin expression was negatively correlated with NKD1 expression (r = -.347, p = .007). The survival time of patients with positive expression of AEG-1 and β-catenin was significantly shortened compared with those with negative expression (p < .05), while the survival time of patients with positive expression of NKD1 was longer than that of patients with negative expression of NKD1 (p < .05).Conclusion: The abnormal expression of AEG-1, NKD-1 and β-catenin is closely related with the occurrence, development, invasion, metastasis and prognosis of  gastric cancer, which provide a therapeutic target and may become markers for prognosis evaluation.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"60 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of improved Beck oral score in oral care of burn patients with mechanical ventilation","authors":"Y. Wang, Rui Guo, Shu-jie Wang, Xiu-mei Zhou","doi":"10.5430/dcc.v10n2p15","DOIUrl":"https://doi.org/10.5430/dcc.v10n2p15","url":null,"abstract":"Objective: To explore the application effect of improved Beck oral score in oral care of burn patients with mechanical ventilation, to provide practical basis for oral care of such patients.Methods: A total of 42 patients with tracheotomy and mechanical ventilation in the Department of Burn Surgery of Baogang Hospital in Inner Mongolia from January 2021 to December 2022 were selected as the study objects, and the patients were randomly divided into observation group and control group, with 21 cases in each group. The control group was given routine oral care, and the observation group was given oral care according to improved Beck oral score. The following were compared between the two groups: Age, total burn area, third-degree burn area, acute physiological and chronic health assessment scores, as well as improved Beck oral scores; the incidence of oral odor, plaque index, mouth ulcer and ventilator-associated pneumonia (VAP) after 7 days; the detection of microorganism in oral secretions.Results: After 7 days, the oral condition of patients in observation group was effectively improved, and the oral comfort level was significantly improved. The incidence of oral odor (2.57±1.59), dental plaque index (2.62±1.47), oral ulcer evaluation (2.24±1.09), VAP incidence (9.52%) and improved Beck oral score (8.09±1.92) in observation group were all lower than those in control group, which was (3.95±1.82), (3.57±1.21), (3.14±1.24), (38.09%), and (9.90±2.21) respectively. The difference was statistically significant (p < .05). The oral normal flora in observation group (71.43%) was higher than that in control group (28.57%), and the difference was statistically significant (p < .05). The oral gram-positive bacteria (19.05%) and Gram-negative bacteria (23.81%) in observation group were lower than those in control group, which was 52.38% and 57.14% respectively, and the difference was statistically significant (p < .05).Conclusions: The application of the improved Beck oral assessment method in dynamic oral care can effectively reduce the content of oral pathogens, lower the incidence of pulmonary infection, and improve the quality of life of patients. ","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"58 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139390072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress in colon cancer surgery","authors":"Aer Sileng, Yueli Yu","doi":"10.5430/dcc.v10n3p1","DOIUrl":"https://doi.org/10.5430/dcc.v10n3p1","url":null,"abstract":"Colon cancer is one of the top three malignant tumors in the world, in recent years, the incidence and mortality of this disease are increasing, which seriously threatens human life and health. At present, surgery is still the main treatment for colon cancer, combined with chemotherapy and other measures can effectively improve the survival of patients. Different surgical methods have different prognostic effects on patients. In terms of intraoperative lymph node dissection for colon cancer, lymph node dissection during total mesocolic resection and D3 radical resection has significantly improved prognosis for colon cancer. Lymph node metastasis of colon cancer is common, with the presence of skip metastasis, lymph node dissection is important for the prognosis of patients, tumor recurrence and tumor metastasis. Therefore, it is necessary to make clear the diagnosis and lymph node metastasis before surgery, determine the surgical procedure and the scope of lymph node dissection, so as to avoid postoperative complications caused by inadequate tumor recurrence or excessive clearance.","PeriodicalId":504730,"journal":{"name":"Discussion of Clinical Cases","volume":"30 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139167082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}