Acta VirologicaPub Date : 2024-08-08DOI: 10.3389/av.2024.13177
Joseph A. Jackson
{"title":"A novel morbillivirus and a novel betaherpesvirus infecting the Wood Mouse in the UK","authors":"Joseph A. Jackson","doi":"10.3389/av.2024.13177","DOIUrl":"https://doi.org/10.3389/av.2024.13177","url":null,"abstract":"A novel morbillivirus and a novel betaherpesvirus are reported in the Wood Mouse (Apodemus sylvaticus) in the western United Kingdom (UK). The two viruses were found coinfecting an underweight host with abnormalities of the liver and were detected via deep sequencing of lung RNA and de novo assembly of substantial genome fragments. The phylogenetic affinities of the novel viruses are characterised based on their relationships to existing database sequences.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"43 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141929665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta VirologicaPub Date : 2024-02-29DOI: 10.3389/av.2024.12515
Yan Wu, Chunying Lu, Ran Zhao, Yuyun He, Jingjing Hou, Yijie Sun, Shixing Yang, Zhaoying Qin, Likai Ji, Yan Wang, Wen Zhang
{"title":"A new bird calicivirus detected in feces of cormorants","authors":"Yan Wu, Chunying Lu, Ran Zhao, Yuyun He, Jingjing Hou, Yijie Sun, Shixing Yang, Zhaoying Qin, Likai Ji, Yan Wang, Wen Zhang","doi":"10.3389/av.2024.12515","DOIUrl":"https://doi.org/10.3389/av.2024.12515","url":null,"abstract":"The incidence of reporting caliciviruses in wild birds is less common than in other animals, and the majority of cases remain unclassified. A strain of calicivirus was discovered in this study in the feces of cormorants collected at Xiamen Horticulture Expo Garden in 2021 and was named Cormcali01. The genome of Cormcali01 was 8,561 bp in length which contained characteristic motifs present in other caliciviruses. Furthermore, it demonstrated a significant deviation from all existing calicivirus nucleotide sequences, exhibiting the highest amino acid identity (47.34%) to the unclassified Ruddy turnstone calicivirus A. A pairwise comparison of the VP1 protein showed that Cormcali01 had the highest amino acid identity of 43.90% with the unassigned Ruddy turnstone calicivirus A. Phylogenetic analysis demonstrated that VP1 of Cormcali01 clustered with unassigned caliciviruses. Therefore, based on phylogenetic analysis and pairwise comparison, Cormcali01 should be affiliated with the unassigned calicivirus, which were suggested to comprise a new calicivirus genus, the Sanovirus genus. After investigating the prevalence of Cormcali01, we discovered that 22.22% of fecal samples (10/45) were tested positive. These findings expand our understanding of the genetic variation of caliciviruses and provide valuable epidemiological information regarding a potential outbreak of calicivirus disease in birds.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"1 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140410178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In-silico design of a new multi-epitope vaccine candidate against SARS-CoV-2","authors":"Abbas Alibakhshi, Armina Alagheband Bahrami, Elmira Mohammadi, Shahrzad Ahangarzadeh, Meysam Mobasheri","doi":"10.3389/av.2023.12481","DOIUrl":"https://doi.org/10.3389/av.2023.12481","url":null,"abstract":"Frequent, and sometimes more dangerous, mutations in SARS-CoV-2 indicate that a stronger strategy is needed to produce an effective vaccine—a vaccine that contains a wider range of virus factors and remains effective if one or more mutations have occurred in a part of the genome. In this study, four important virus proteins were used to make a multi-epitope protein vaccine. For this purpose, antigenic determinant of 4 proteins were selected and a protein structure was designed using 4 domains containing epitopes. After examining its antigenic potential, its three-dimensional structure was designed and then docked with immune system receptors. Finally, using the dynamic molecular (MD) simulation, complexes and interactions were investigated and their interaction energies were measured. The results of the study showed that the designed structure has good relative stability and interacts well with its receptors and can be used as a vaccine candidate for further studies.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"56 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139386693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta VirologicaPub Date : 2023-11-21DOI: 10.3389/av.2023.12075
J. Biswal, J. Mohapatra, Rajeev Ranjan, M. Rout, Shyam Singh Dahiya, Rabindra Prasad Singh
{"title":"TaqMan probe-based one-step multiplex real-time RT-PCR assay for the diagnosis of foot-and-mouth disease","authors":"J. Biswal, J. Mohapatra, Rajeev Ranjan, M. Rout, Shyam Singh Dahiya, Rabindra Prasad Singh","doi":"10.3389/av.2023.12075","DOIUrl":"https://doi.org/10.3389/av.2023.12075","url":null,"abstract":"Effective control and monitoring the spread of foot-and-mouth disease (FMD) relies upon rapid and accurate laboratory detection of FMD virus (FMDV). Therefore, in this report, a multiplex TaqMan probe-based one-step RT-qPCR assay simultaneously targeting FMDV 5′UTR and 3Dpol regions, and 18S rRNA housekeeping gene (as an internal control) in a single reaction tube was developed and evaluated. The multiplex one-step RT-qPCR assay specifically detected viral genome in both FMDV-infected cell culture suspensions and clinical samples collected from known-FMD infected animals. The assay could detect FMDV RNA in the archived FMDV cell culture isolates (n = 120) collected during the last two decades in India. In addition, the new assay could also detect viral RNA in the FMD suspected clinical samples (n = 740) collected from various field outbreaks. At a cut-off Ct-value of <38, the assay could detect at least 20 and 10 copies of FMDV 3Dpol and 5′UTR genes, respectively. Further, the multiplex RT-qPCR assay proved to be robust, showing an inter-assay co-efficient of variations ranging from 1.3% to 3.03% for FMDV-3Dpol gene target, and from 1.44% to 4.69% for 5′UTR gene target. In addition, it was found that the new assay could be used to detect viral genome in a variety of samples (epithelium, saliva, OPF, milk and blood) without any significance difference in the detection limit of the assay. Hence, the multiplex one-step RT-qPCR assay could be considered a valuable tool for the detection of FMDV in India.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"49 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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