Acta Virologica最新文献

Nutlin-3 promotes HBV replication by activating mTOR-mediated autophagy Nutlin-3通过激活mtor介导的自噬来促进HBV复制

Acta Virologica Pub Date : 2024-11-22 DOI: 10.3389/av.2024.12585

Shaowei Han, Yuanyuan Li, Junnan Wang, Yong Zhang

{"title":"Nutlin-3 promotes HBV replication by activating mTOR-mediated autophagy","authors":"Shaowei Han, Yuanyuan Li, Junnan Wang, Yong Zhang","doi":"10.3389/av.2024.12585","DOIUrl":"https://doi.org/10.3389/av.2024.12585","url":null,"abstract":"Background Currently, there are approximately 240 million individuals worldwide suffering from chronic hepatitis B infection, yet a definitive cure remains elusive. In the context of pharmaceutical interventions for this patient population, the potential issue of Hepatitis B virus (HBV) reactivation must be taken into consideration. Nutlin-3 is a small molecule of imidazoline analog, which inhibits tumor growth by suppressing the interaction between human double minute 2 (HDM2) and p53. However, whether it affects HBV replication is not clear. Methods HepG2.2.15 cells were used to assess the impact of Nutlin-3 on HBV replication. The expression of Hepatitis B surface antigen (HBsAg) and Hepatitis B e antigen (HBeAg) was detected by ELISA. The expression of pregenomic RNA (pgRNA) and core-DNA was detected by RT-qPCR. The activation of relevant signaling pathways was assessed through Western blotting and confocal microscopy detection of the expression of associated proteins. Results We found that Nutlin-3 significantly enhances the expression of HBsAg and HBeAg, and it also promotes the expression of pgRNA and Core-DNA. Nutlin-3 promotes HBV replication independent of the HDM2. Instead, it activates the autophagy pathway through p53-mediated mTOR inhibition to promote HBV replication. Conclusion When using Nutlin-3 for cancer therapy, it’s important to be aware of the potential side effect of promoting HBV replication in individuals with chronic hepatitis B infection.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://public-pages-files-2025.frontierspartnerships.org/journals/acta-virologica/articles/10.3389/av.2024.12585/pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147914188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duck enteritis virus UL7 is a late gene and the UL7-encoded protein co-localizes with pUL51 鸭肠炎病毒UL7是一种晚期基因，UL7编码的蛋白与pUL51共定位

Acta Virologica Pub Date : 2024-08-16 DOI: 10.3389/av.2024.12023

Jie Huang, Mingshu Wang, Anchun Cheng, Bin Tian, Xuming Ou, Ying Wu, Qiao Yang, Di Sun, Shaqiu Zhang, Sai Mao, Xinxin Zhao, Juan Huang, Qun Gao, Dekang Zhu, Renyong Jia, Shun Chen, Mafeng Liu

引用次数: 0

A novel morbillivirus and a novel betaherpesvirus infecting the Wood Mouse in the UK 感染英国木鼠的一种新型变形病毒和一种新型β疱疹病毒

Acta Virologica Pub Date : 2024-08-08 DOI: 10.3389/av.2024.13177

Joseph A. Jackson

引用次数: 0

A new bird calicivirus detected in feces of cormorants 在鸬鹚粪便中检测到一种新的鸟类卡里科病毒

Acta Virologica Pub Date : 2024-02-29 DOI: 10.3389/av.2024.12515

Yan Wu, Chunying Lu, Ran Zhao, Yuyun He, Jingjing Hou, Yijie Sun, Shixing Yang, Zhaoying Qin, Likai Ji, Yan Wang, Wen Zhang

{"title":"A new bird calicivirus detected in feces of cormorants","authors":"Yan Wu, Chunying Lu, Ran Zhao, Yuyun He, Jingjing Hou, Yijie Sun, Shixing Yang, Zhaoying Qin, Likai Ji, Yan Wang, Wen Zhang","doi":"10.3389/av.2024.12515","DOIUrl":"https://doi.org/10.3389/av.2024.12515","url":null,"abstract":"The incidence of reporting caliciviruses in wild birds is less common than in other animals, and the majority of cases remain unclassified. A strain of calicivirus was discovered in this study in the feces of cormorants collected at Xiamen Horticulture Expo Garden in 2021 and was named Cormcali01. The genome of Cormcali01 was 8,561 bp in length which contained characteristic motifs present in other caliciviruses. Furthermore, it demonstrated a significant deviation from all existing calicivirus nucleotide sequences, exhibiting the highest amino acid identity (47.34%) to the unclassified Ruddy turnstone calicivirus A. A pairwise comparison of the VP1 protein showed that Cormcali01 had the highest amino acid identity of 43.90% with the unassigned Ruddy turnstone calicivirus A. Phylogenetic analysis demonstrated that VP1 of Cormcali01 clustered with unassigned caliciviruses. Therefore, based on phylogenetic analysis and pairwise comparison, Cormcali01 should be affiliated with the unassigned calicivirus, which were suggested to comprise a new calicivirus genus, the Sanovirus genus. After investigating the prevalence of Cormcali01, we discovered that 22.22% of fecal samples (10/45) were tested positive. These findings expand our understanding of the genetic variation of caliciviruses and provide valuable epidemiological information regarding a potential outbreak of calicivirus disease in birds.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"1 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140410178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico design of a new multi-epitope vaccine candidate against SARS-CoV-2 针对 SARS-CoV-2 的新型多表位候选疫苗的硅内设计

Acta Virologica Pub Date : 2024-01-04 DOI: 10.3389/av.2023.12481

Abbas Alibakhshi, Armina Alagheband Bahrami, Elmira Mohammadi, Shahrzad Ahangarzadeh, Meysam Mobasheri

引用次数: 0

TaqMan probe-based one-step multiplex real-time RT-PCR assay for the diagnosis of foot-and-mouth disease 基于 TaqMan 探针的一步法多重实时 RT-PCR 分析法用于口蹄疫诊断

Acta Virologica Pub Date : 2023-11-21 DOI: 10.3389/av.2023.12075

J. Biswal, J. Mohapatra, Rajeev Ranjan, M. Rout, Shyam Singh Dahiya, Rabindra Prasad Singh

{"title":"TaqMan probe-based one-step multiplex real-time RT-PCR assay for the diagnosis of foot-and-mouth disease","authors":"J. Biswal, J. Mohapatra, Rajeev Ranjan, M. Rout, Shyam Singh Dahiya, Rabindra Prasad Singh","doi":"10.3389/av.2023.12075","DOIUrl":"https://doi.org/10.3389/av.2023.12075","url":null,"abstract":"Effective control and monitoring the spread of foot-and-mouth disease (FMD) relies upon rapid and accurate laboratory detection of FMD virus (FMDV). Therefore, in this report, a multiplex TaqMan probe-based one-step RT-qPCR assay simultaneously targeting FMDV 5′UTR and 3Dpol regions, and 18S rRNA housekeeping gene (as an internal control) in a single reaction tube was developed and evaluated. The multiplex one-step RT-qPCR assay specifically detected viral genome in both FMDV-infected cell culture suspensions and clinical samples collected from known-FMD infected animals. The assay could detect FMDV RNA in the archived FMDV cell culture isolates (n = 120) collected during the last two decades in India. In addition, the new assay could also detect viral RNA in the FMD suspected clinical samples (n = 740) collected from various field outbreaks. At a cut-off Ct-value of <38, the assay could detect at least 20 and 10 copies of FMDV 3Dpol and 5′UTR genes, respectively. Further, the multiplex RT-qPCR assay proved to be robust, showing an inter-assay co-efficient of variations ranging from 1.3% to 3.03% for FMDV-3Dpol gene target, and from 1.44% to 4.69% for 5′UTR gene target. In addition, it was found that the new assay could be used to detect viral genome in a variety of samples (epithelium, saliva, OPF, milk and blood) without any significance difference in the detection limit of the assay. Hence, the multiplex one-step RT-qPCR assay could be considered a valuable tool for the detection of FMDV in India.","PeriodicalId":502607,"journal":{"name":"Acta Virologica","volume":"49 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0