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[PLANT GENETIC TRANSFORMATION USING CARBON NANOTUBES FOR DNA DELIVERY]. [利用碳纳米管传递 DNA 的植物基因转化]。
TSitologiia i genetika Pub Date : 2015-11-01
O M Burlaka, Ya V Pirko, A I Yemets, Ya B Blume
{"title":"[PLANT GENETIC TRANSFORMATION USING CARBON NANOTUBES FOR DNA DELIVERY].","authors":"O M Burlaka, Ya V Pirko, A I Yemets, Ya B Blume","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The possibility of exploiting carbon nanotubes (CNTs)-based nanocarriers to deliver genes into protoplasts, callus and mesophyll explants of plants was examined. Using single-walled CNTs (SWCNTs) at the concentration of 20 μg/ml and multi-walled CNTs (MWCNTs) at the concentration of 15 μg/ml genetic transformation of Nicotiana tabacum L. mesophyll protoplasts with plasmid pGreen 0029 was carried out and transient expression of reporter yfp gene in the protoplasts was observed. Using SWCNTs at the concentration of 40 μg/ml and MWCNTs at the concentration of 30 μg/ml genetic transformation of N. tabacum callus and leaf explants with nptII gene as a part of plasmid pGreen 0029 was carried out. As a result plant regeneration on selective medium containing 50 mg/lkanamycin was shown. SWCNTs-based nanocarriers de-onstrated their appli-ability to transform protoplasts as well as walled plant cells. Whereas, MWCNTs-based nano-arriers were suitable only for transformation of proto-lasts due to the limiting role of cellulose walls in cell penetration.</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"49 6","pages":"3-12"},"PeriodicalIF":0.0,"publicationDate":"2015-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
OBTAINING OF THE TRANSGENIC HELIANTHUS TUBEROSUS L. PLANTS, CALLUS AND "HAIRY" ROOT CULTURES ABLE TO EXPRESS THE RECOMBINANT HUMAN INTERFERON ALPHA-2b GENE. 获得能够表达重组人类干扰素 ALPHA-2b 基因的转基因 HELIANTHUS TUBEROSUS L. 植物、胼胝体和 "毛状 "根培养物。
TSitologiia i genetika Pub Date : 2015-09-01
O M Maistrenko, Yu S Luchakivska, N M Zholobak, M Ya Spivak, M V Kuchuk
{"title":"OBTAINING OF THE TRANSGENIC HELIANTHUS TUBEROSUS L. PLANTS, CALLUS AND \"HAIRY\" ROOT CULTURES ABLE TO EXPRESS THE RECOMBINANT HUMAN INTERFERON ALPHA-2b GENE.","authors":"O M Maistrenko, Yu S Luchakivska, N M Zholobak, M Ya Spivak, M V Kuchuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This work is the first to our knowledge to describe the successful attempt of Agrobacterium rhizogenes-mediated transformation of topinambour in order to obtain the transgenic H. tuberosus plants, callus and \"hairy\" root cultures. The plasmid vectors contained the sequence of interferon gene fused with Nicotiana plumbagenifolia L. calreticulin apoplast targeting signal driven by 35S CaMV promoter or root-specific Mll promoter. Nearly 75% isolated Ri-root lines and callus cultures were proved (by PCR analysis) to contain HuINFa-2b transgene. We also managed to obtain H. tuberosus transgenic plants through somatic embryogenesis on the transgenic \"hairy\" root culture. The obtained transgenic H. tuberosus cultures exhibited high-level antiviral activity that ranged from 2000 to 54500 IU/g FW that makes this crop considered a promising source of recombinant interferon alpha 2b protein.</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"49 5","pages":"38-44"},"PeriodicalIF":0.0,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Peculiarities of the influence of chemical and physical factors on cytogenetic indices of root meristem in Pisum sativum L]. [化学和物理因素对豌豆根分生组织细胞遗传指数影响的特殊性]。
TSitologiia i genetika Pub Date : 2002-05-01
S S Rudenko, T V Morozova, S S Kostyshyn, Ie D Strel'chenko
{"title":"[Peculiarities of the influence of chemical and physical factors on cytogenetic indices of root meristem in Pisum sativum L].","authors":"S S Rudenko, T V Morozova, S S Kostyshyn, Ie D Strel'chenko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The results of comparative analysis of cytogenetic effects of various chemical and physical factors on meristemic cells of field pea (Pisum sativum L.) are presented. Cytogenetic effects of aluminum, cadmium and selenium compounds as well as X-ray irradiation were studied. Cytogenetic indices were investigated for different doses of the above factors and peculiarities of their influence on the ratio of mitotic phases were analyzed. The increasing in the proportion of cells at the specific mitotic stage was revealed. There were anaphase stage for aluminum chloride, prophase stage for cadmium chloride, meta- and anaphase stages for sodium selenate, and telophase stage for X-rays. Investigated chemical elements may be ranged according to their ability to induce the aberrant anaphase frequency in the row: Se (from 3.75 x 10(-6) M) > A1 (from 3.86 x 10(-5) M) > Cd (from 8.44 x 10(-5) M). Maximal experimental dose of X-ray irradiation (9.03 x 10(-3) C/kg) induces the frequency of chromosome aberration similar to those induced by maximal experimental doses of aluminum and selenium (3.86 x 10(-4) M and 8.34 x 10(-6) M).</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"36 3","pages":"22-7"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Molecular and genetic polymorphism of cultivated soybean detected by AP-PCR, SSRP and SSR]. [利用 AP-PCR、SSRP 和 SSR 检测栽培大豆的分子和遗传多态性]。
TSitologiia i genetika Pub Date : 2001-09-01
A F Brik, Iu M Sivolap
{"title":"[Molecular and genetic polymorphism of cultivated soybean detected by AP-PCR, SSRP and SSR].","authors":"A F Brik, Iu M Sivolap","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Molecular-genetic polymorphism of cultivated soybean from different geographic regions was analysed using three PCR methods (AP-PCR, SSRP and ISSR). The dendrogram of phenogenetic relationship was constructed on the base of obtained data. The allocation of varieties are in a good agreement with pedigree information. Main parameters such as polymorphism level, expected heterozygosity, and marker index were calculated. It was shown that ISSR is the method of choice for the differentiation of soybean varieties.</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"35 5","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2001-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Variation of ultrastructural organization of the mitochondria of pea cells in vitro]. [体外豌豆细胞线粒体超微结构组织的变化]。
TSitologiia i genetika Pub Date : 2001-03-01
D O Klimchuk
{"title":"[Variation of ultrastructural organization of the mitochondria of pea cells in vitro].","authors":"D O Klimchuk","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Unusual huge organelles together with mitochondria of typical organization were found in a part of population of callus cells of pea. They like mitochondria are surrounded by two biologic membranes, the inner one has invaginations. Inclusions in the matrix are a specific character of structural organization of these unusual mitochondria. These inclusions look as electronically dense bars that extend parallel to longitudinal axis of organelle and consist of fibril-like elements. Organization of inclusions and cristae as well as a shape of organelles are changing during cell development. Possible nature of the unusual mitochondria is discussed.</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"35 2","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2001-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[T-DNA induced anomalies of flowers and male sterility in transgenic tobacco plants: morphometric and cytological analysis]. [T-DNA诱导的转基因烟草植物花朵异常和雄性不育：形态计量学和细胞学分析]。
TSitologiia i genetika Pub Date : 2000-11-01
Iu V Sidorchuk, A A Zagorskaia, E V Deĭneko, N V Shamina, V K Shumnyĭ
{"title":"[T-DNA induced anomalies of flowers and male sterility in transgenic tobacco plants: morphometric and cytological analysis].","authors":"Iu V Sidorchuk, A A Zagorskaia, E V Deĭneko, N V Shamina, V K Shumnyĭ","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Morphometric and cytological analysis have been carried out in transgenic tobacco (Nicotiana tabacum L.) plants possessing male sterility and abnormalities of flowers. Mutation affected flower structure and conducted to long pistil and male sterility. It was established that development of microspores was blockaded on different stages of gametogenesis but the first injuries were detected for tetrad stage.</p>","PeriodicalId":502127,"journal":{"name":"TSitologiia i genetika","volume":"34 6","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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