{"title":"Essential Oils and Organic Acid Blends Potentially Enhance Growth and Ameliorate Enteritis and Inflammatory Response After Challenge with Clostridium perfringens in Chickens","authors":"Ghada Abd El-Dayem, Nesma Rasheed, Rania Ghanem, Ghadeer Albadrani, Mohamed Abdel-Daim, Mervat Abdel-Latif","doi":"10.21608/djvs.2023.230008.1120","DOIUrl":"https://doi.org/10.21608/djvs.2023.230008.1120","url":null,"abstract":": Single or combined essential oils (EOs), and organic acids (OAs) blends were used as continuous drinking water (DW) treatment for protection against Clostridium perfringens ( C. perfringens ) experimental infection in chickens. Random assignment of 185 one-day-old, commercial broiler chicks into 6 groups (30 chicks/group in 3 replicates) arranged as follows: a non-infected group (G1), C. perfringens infected (G2). In contrast, G3, and G4 were infected with C. perfringens and continuously supplemented with EOs, and OAs, respectively in DW from day 1. Chickens in G5 were challenged with C. perfringens and had a mixture of both products continuously in DW from day 1. At the same time, the birds of G6 were challenged with C. perfringens and treated with amoxicillin. All groups were fed a basal diet. Our results showed that combined EO and OA blends were more effective in ameliorating the devastating effects of C. perfringens in broilers than single treatments. In the infected-treated groups, the growth performance represented by body weight gain, and feed conversion ratio were improved ( p < 0.05), intestinal lesion scores, and C. perfringens count were significantly low. Also, the intestinal histopathology and biochemical profile were improved. In addition, iNOS and IL-1 β (pro-inflammatory cytokines) levels were significantly down regulated while IL-10 (anti-inflammatory cytokine) was increased ( p < 0.05). Furthermore, the phagocytic activity was significantly ( p < 0.05) enhanced. In conclusion, EO and OA blends significantly improved the broiler chickens’ performance, inflammatory, and immune response and reduced intestinal lesions. A marked difference occurred in favor of combined than single treatments compared to antibiotic treatment facing the C. perfringens ’ infection.","PeriodicalId":496466,"journal":{"name":"Damanhour Journal of Veterinary Sciences (Online)","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135788606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yousra Nosair, Moustafa Kassem, Ahmed Nomir, Mohamed El-Sherif, Ahmed El-Khamary
{"title":"The Value of Thoracolumbar Radiography for the Judgment of Horse Soundness and the Pre-purchase Requirement","authors":"Yousra Nosair, Moustafa Kassem, Ahmed Nomir, Mohamed El-Sherif, Ahmed El-Khamary","doi":"10.21608/djvs.2023.206616.1113","DOIUrl":"https://doi.org/10.21608/djvs.2023.206616.1113","url":null,"abstract":"Background: Back pain is a disorder with a high-frequency recurrence in horses. There are absolute indications to request radiography besides physical, dynamic, and lameness examinations, especially in animals with normal appearance and without lameness undergoing alteration of the thoracolumbar spines and the sudden onset of back pain. Objective: To assess the value of radiography in identifying thoracolumbar conditions in normal equine that may affect the soundness of the animal and its significance as a pre-purchase basic exam. To determine how well radiography can find thoracolumbar conditions in horses that seem healthy but could affect the animal's health and how important it is as a basic pre-purchase exam. Methods: Nine horses of both sexes, 2 of them were showing lameness, and 7 were apparently normal (lameness-free). A series of lateromedial and DM 20° VLO radiographs of the thoracolumbar column (T1-L6) were acquired with digital equipment. The images were analyzed, and radiographic findings were noted. Results: Different osseous lesions were noted among the studied horses groups. Variable degrees of bone lysis, bone cyst-like lesions and bone remodelling were observed. Kissing spines, sclerosis, and APJ ankylosis were also detected. Though the animals suffer no lameness, radiography predicts their future unsoundness. Conclusion: A radiographic examination of the thoracolumbar spine should be a basic annual exam and a fundamental pre-purchasing procedure to predict an animal's future back pain and lameness.","PeriodicalId":496466,"journal":{"name":"Damanhour Journal of Veterinary Sciences (Online)","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135081931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production and evaluation of rabbit IgG against IBDV in broiler chickens","authors":"Suzan Dabour, Eid Hussein, Madiha Ibrahim, Emad Elgendy","doi":"10.21608/djvs.2023.199166.1111","DOIUrl":"https://doi.org/10.21608/djvs.2023.199166.1111","url":null,"abstract":": Infectious bursal disease is an acute highly contagious disease affecting young chickens causing increased mortality and severe immunosuppression. Therefore, additional approaches together with vaccination are required to overcome its endemicity. Here, ten males Bosch rabbit of 3 months old (2-3 kg/ weight) were used for production of IgG against IBDV. The produced rabbit IgGs were titrated using Passive Hemagglutination (PHA) and evaluated experimentally for their protection against vvIBDV challenge in commercial broiler chickens. Daily observation of clinical signs, mortalities and postmortem changes were recorded till 10 days post-infection. The spleen and bursa were collected for histopathological examination and cloacal swabs were collected to evaluate viral shedding by real time-PCR. The results indicated the effectiveness of the anti-vvIBDV IgG in protection against vvIBDV and in the reduction of viral shedding. These results suggested that IgG produced in rabbits may help in IBDV control and decrease its commercial economic losses.","PeriodicalId":496466,"journal":{"name":"Damanhour Journal of Veterinary Sciences (Online)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135204816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular Prevalence and Risk Factors Analysis of Asymptomatic Infection with Anaplasma marginale and Theileria annulata in Egyptian Cattle","authors":"Samy Metwally, Nabil Bakeir, Yassien Badr, Mina Saad, Besheer Elshafey, Wael Goda, Rania Hamada","doi":"10.21608/djvs.2023.220121.1117","DOIUrl":"https://doi.org/10.21608/djvs.2023.220121.1117","url":null,"abstract":"Awareness of the epidemiology of Anaplasma marginale ( A. marginale ) and Theileria annulata ( T. annulata ) in the asymptomatic carriers is integral for developing efficient control in tropical and subtropical countries. The endemic status of both pathogens among asymptomatic carrier cattle in Egypt is poorly investigated. This study was conducted to detect the prevalence of A. marginale and T. annulata infections in apparently healthy cattle from six Egyptian provinces based on specific polymerase chain reaction (PCR) assays and to assess its associated risk factors. Two hundred seventy-eight blood samples were collected from cattle in 24 farms and four abattoirs in Beheira, Damietta, Cairo, Fayoum, Qena, and Luxor. DNA was extracted, and A. marginale msp4 and T. annulata tams-1 genes were amplified using Real-Time PCR assays. The overall prevalence of A. marginale and T. annulata were 39.6% and 5.8%, respectively. Meanwhile, the prevalence of co-infection was 2.5%. The prevalence of A. marginale (0.0% - 65.1%) and T. annulata (0.0% - 25.0%) greatly varied between provinces. Statistical analysis of risk factors indicated that female cows (49.7%; OR = 3.4; p = < 0.0001), Mixed breed (47.4%; OR = 3.6; p = 0.03), dairy (48.2%; OR = 3.7; p = < 0.0001), and small-size farms (60.2%; OR = 4.6; p = < 0.0001) showed a significantly higher risk for A. marginale compared to the references. Meanwhile, for T. annulata, cattle from Luxor (25.0%; OR = 10.3; p = 0.03) and ages < 2 years (16.7%; OR = 4.6; p = 0.038) had a significantly higher risk against the references. These findings highlight the endemic status of A. marginale and T. annulata in asymptomatic cattle and analyze the risks influencing their prevalence in Egyptian cattle.","PeriodicalId":496466,"journal":{"name":"Damanhour Journal of Veterinary Sciences (Online)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134997321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}