Elidia Zotelli dos Santos, Herbert Souza Soares, Stephanie Rodrigues dos Santos, Jonas Moraes Filho, Hilda Fátima de Jesus Pena, Marcos Amaku, Solange Maria Gennari
{"title":"Toxoplasma gondii and Neospora caninum antibody seroprevalence and risk factors among dogs treated at Public Veterinary Hospitals in São Paulo, Brazil","authors":"Elidia Zotelli dos Santos, Herbert Souza Soares, Stephanie Rodrigues dos Santos, Jonas Moraes Filho, Hilda Fátima de Jesus Pena, Marcos Amaku, Solange Maria Gennari","doi":"10.1590/s1984-29612023058","DOIUrl":"https://doi.org/10.1590/s1984-29612023058","url":null,"abstract":"Abstract Dogs can be infected by Toxoplasma gondii and Neospora caninum, for which they function, respectively, as intermediate, and definitive hosts. In the present study seroprevalence against T. gondii and N. caninum antibodies, were determined by indirect fluorescent antibody test (cut off of 16 and 50, respectively), in dogs that were treated at public veterinary hospitals in the metropolitan region of São Paulo and risk factors were identified. Out of the 1,194 samples 125 (10.5%; 95% CI: 8.8-12.3%) were positive for T. gondii and 9 (0.75%, 95% CI: 0.34-1.4%) for N. caninum. For T. gondii, statistical differences were observed between the proportions of positive dogs and different zones of the municipality (p = 0.025), and age (p = 0.02), higher among older dogs. The keepers were invited to answer an epidemiological questionnaire to analyze risk factors, and 471 (39.4%) agreed to be interviewed, and among their dogs 65 (13.8%) were T. gondii seropositive. Age group above 8 years (OR = 3.63; 95% CI: 1.08-12.23) was a risk factor and having a defined breed (OR = 0.49; 95% CI: 0.25-0.96) was a protective factor for T. gondii infection. Because of the low number of dogs positive for N. caninum, risk factors for this coccidium were not determined.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135609057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Agostinho Sergio Scofano, Igor Falco Arruda, Jessica Nogueira Teixeira, Nathalie Costa da Cunha, Elmiro Rosendo do Nascimento, Maria Regina Reis Amendoeira, Patrícia Riddell Millar
{"title":"Toxoplasma gondii infection in pig intended for human consumption: seroprevalence, risk factors and influence of biosecurity measures","authors":"Agostinho Sergio Scofano, Igor Falco Arruda, Jessica Nogueira Teixeira, Nathalie Costa da Cunha, Elmiro Rosendo do Nascimento, Maria Regina Reis Amendoeira, Patrícia Riddell Millar","doi":"10.1590/s1984-29612023060","DOIUrl":"https://doi.org/10.1590/s1984-29612023060","url":null,"abstract":"Abstract A serologic and epidemiologic study was carried out in order to determinate herd and animal seroprevalence and associated factors for Toxoplasma gondii in commercial pigs from Espírito Santo state, Brazil. Blood samples were collected from 416 pigs from 55 producer farms in 27 municipalities. An indirect immunofluorescent assay (IFA) was performed to estimate the seroprevalence of T. gondii and identify the associated risk factors using a questionnaire. The T. gondii antibody prevalence rate in commercial swine herds was 15.4% (64/416) using a cutoff of 1:64. The seropositivity for T. gondii was related to the presence of cats, water origin and age of swine in the increase of seroprevalence, and the existence of internal isolation fences and use of composting chambers as protective factors. To the best of our knowledge, this is the first study to report anti- T. gondii antibodies in the serum of pigs in the state of Espírito Santo, Brazil. This finding is important to public health because seropositive pigs can harbor tissue cysts in their meat, thereby representing a zoonotic risk for consumers of raw or undercooked porcine meat or its products.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135659440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological identification of Skrjabinisakis Mozgovoi, 1951 (Nematoda: Anisakidae) in Kogia sima (Cetacea: Kogiidae) from Brazilian waters","authors":"Raul Henrique da Silva Pinheiro, Andréa Magalhães Bezerra, Elane Guerreiro Giese","doi":"10.1590/s1984-29612023064","DOIUrl":"https://doi.org/10.1590/s1984-29612023064","url":null,"abstract":"Abstract New morphological, morphometric and scanning electron microscopy data of a nematode of the family Anisakidae, recovered from a specimen of Kogia sima, a cetacean that died off the northern coast of Brazil, are presented in this paper. Morphological features such as the violin-shaped ventricle and short and equal spicules, as well as the distribution of post-cloacal papillae and specificity for the definitive host (Kogiidae cetaceans) demonstrate similarity to Skrjabinisakis paggiae. This research records Kogia sima and S. paggiae on the estuarine coast of Pará, northern Brazil.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135660008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathalia Delucinhore Lima, D. C. Raimundo, V. D. Souza, Juliana Martins Aguiar
{"title":"Occurrence of gastrointestinal parasites in dogs and cats domiciliated in Santos, SP, Brazil","authors":"Nathalia Delucinhore Lima, D. C. Raimundo, V. D. Souza, Juliana Martins Aguiar","doi":"10.1590/S1984-29612021080","DOIUrl":"https://doi.org/10.1590/S1984-29612021080","url":null,"abstract":"Abstract Helminths and protozoa are major causes of diseases in domestic animals, and many can also cause infections in humans. Knowledge of intestinal parasitoses affecting domestic animals is important for the implementation of appropriate preventive measures. The objective of this study was to evaluate the occurrence of gastrointestinal parasites in fecal samples of dogs and cats attended at the Veterinary Hospital of the Metropolitan University of Santos, SP, Brazil. We also attempted to determine whether such infection was associated with sex, age, or the presence of diarrhea. We analyzed 100 fecal samples: 85 from dogs and 15 from cats. Among the dogs, 31.8% of the samples were positive, and to 40.0% among the cats. Infection was not associated with sex or age. However, among the dogs, parasitism showed a significant association with the presence of diarrhea (P = 0.013). The helminths Ancylostoma spp. and the protozoa Giardia duodenalis were the most frequent parasites in this research. Although they present unknown species and assemblages, they are parasites with a zoonotic potential of great importance in public health. Therefore, it is essential that pets are properly diagnosed and treated against gastrointestinal parasitic infection to prevent the spread of diseases.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"30 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83312525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. S. Cavalcante, S. Morais, W. André, J. V. Araújo-Filho, C. Muniz, L. O. Rocha, W. Ribeiro, Ana Livya Moreira Rodrigues, L. Oliveira, C. Bevilaqua, M. Ramos
{"title":"Chemical constituents of Calotropis procera latex and ultrastructural effects on Haemonchus contortus","authors":"G. S. Cavalcante, S. Morais, W. André, J. V. Araújo-Filho, C. Muniz, L. O. Rocha, W. Ribeiro, Ana Livya Moreira Rodrigues, L. Oliveira, C. Bevilaqua, M. Ramos","doi":"10.1590/s1984-29612020045","DOIUrl":"https://doi.org/10.1590/s1984-29612020045","url":null,"abstract":"Abstract This study aimed to evaluate the anthelmintic and ultrastructural effects of Calotropis procera latex on Haemonchus contortus. C. procera latex was twice centrifuged at 10,000×g and dialyzed to obtain a fraction rich in proteins, named LP (latex protein), and at 3,000 rpm to obtain a fraction rich in secondary metabolites, named LNP (latex non-protein). Specimens of H. contortus exposed to LNP, LP and PBS in the Adult Worm Motility Test (AWMT) were submitted to scanning (SEM) and transmission (TEM) electron microscopy to verify changes in their ultrastructure. Phytochemical tests in the LNP indicated the presence of phenols, steroids, alkaloids and cardenolides. High-Performance Liquid Chromatography (HPLC) characterized the presence of the compounds gallic acid and quercetin in the LNP. The protein content in the LP was 43.1 ± 1.1 mg/mL and 7.7 ± 0.3 mg/mL in LNP. In AWMT, LNP and LP inhibited the motility of 100% of the nematodes, with LNP being more effective than LP and ivermectin more effective than both (p <0.05). Cuticle changes were observed by SEM and TEM in nematodes treated with LP and LNP. Calotropis procera latex has anthelmintic effects against H. contortus, causing damage to its cuticle and other alterations in its ultrastructure.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"1 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. D. J. E. Dias, S. C. D. S. Clemente, Marcelo Knoff
{"title":"Nematoides anisaquídeos e cestoides Trypanorhyncha de importância em saúde pública em Aluterus monoceros (Linnaeus, 1758) no Estado do Rio de Janeiro, Brasil","authors":"F. D. J. E. Dias, S. C. D. S. Clemente, Marcelo Knoff","doi":"10.1590/S1984-29612010000200005","DOIUrl":"https://doi.org/10.1590/S1984-29612010000200005","url":null,"abstract":"One hundred specimens of unicorn leatherjacket, Aluterus monoceros purchased from markets of municipalities of Niteroi and Rio de Janeiro from May to August 2006. The fishes were measured, necropsied, fileted and analysed their organs. Sixteen fishes were parasitized by nematode Anisakidae: Anisakis spp. and Contracaecum sp. with respectively, 1 and 16% of prevalence, 2 and 3.31 of mean intensity, and 0.02 and 0.53 of mean abundance. Two larvae of Anisakis sp. were found in mesentery of one fish and Contracaecum sp. was found in liver and mesentery with 1 to 9 specimens of range of infection. Fifty-one fishes were parasitized on the liver and mesentery by metacestodes of Trypanorhyncha. The collected species were Floriceps saccatus and Callitetrarhynchus speciosus with respectively, 45 and 6% of prevalence, 3.17 and 2.83 of mean intensity, and 1.43 and 0.06 of mean abundance, the range of infection by F. saccatus was 1 to 20 and by C. speciosus was 1 to 5. Anisakis sp. and these two species of Trypanothyncha were reported in A. monoceros for the first time.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"72 1","pages":"94-97"},"PeriodicalIF":1.3,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90581656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diana Cuglovici Abrão, S. Abrão, C. H. C. Viana, C. R. Valle
{"title":"Utilização do método Famacha no diagnóstico clínico individual de haemoncose em ovinos no Sudoeste do Estado de Minas Gerais","authors":"Diana Cuglovici Abrão, S. Abrão, C. H. C. Viana, C. R. Valle","doi":"10.1590/S1984-29612010000100014","DOIUrl":"https://doi.org/10.1590/S1984-29612010000100014","url":null,"abstract":"The aim of this study was to evaluate the efficacy of Famacha method in individual clinical diagnosis of haemonchosis in sheep from the south-west of Minas Gerais. Fifty crossbred Santa Ines ewes were evaluated during 5 months as for conjunctival mucous colour (Famacha method), Packed Cell Volume (PCV), Faecal Egg Counts (FEC), body score and clinic alterations. The score Famacha observed was from 1 to 3, the body score from 2 to 5, the PCV from 20 to 47%, and FEC from 0 to 7,750. The correlations between the parameters evaluated, except Famacha with body score, were highly significant (P < 0.0001). The main genus identified in the coprocultures was Haemonchus (87 - 100% of total larvae, ± 93.5%). We observed reduction of 87.33% in the use of anthelmintics on the property. It was concluded that the Famacha method, associated with other diagnostic methods, is a useful tool to detect hemonchosis in sheep.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"57 1","pages":"68-70"},"PeriodicalIF":1.3,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88508557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toxoplasma gondii: diagnóstico da infecção experimental e natural em pombos (Columba livia) por técnicas sorológicas, biológicas e moleculares.","authors":"Fernanda Sartori Lima de Godoi","doi":"10.11606/D.42.2009.TDE-01022010-094111","DOIUrl":"https://doi.org/10.11606/D.42.2009.TDE-01022010-094111","url":null,"abstract":"","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"48 1","pages":"237-243"},"PeriodicalIF":1.3,"publicationDate":"2009-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77708895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedro Paulo da Costa Alves Filho, L. Barros, J. O. Campos, Vania Braga, I. Cazorla, George Rêgo Albuquerque, S. Carvalho
{"title":"Parasitas zoonóticos em fezes de cães em praças públicas do município de Itabuna, Bahia, Brasil","authors":"Pedro Paulo da Costa Alves Filho, L. Barros, J. O. Campos, Vania Braga, I. Cazorla, George Rêgo Albuquerque, S. Carvalho","doi":"10.1590/S1984-29612008000400007","DOIUrl":"https://doi.org/10.1590/S1984-29612008000400007","url":null,"abstract":"The contamination of public squares by zoonotic potential parasites was evaluated at the urban areas in the municipality of Itabuna Brasil. For such, 119 fecal samples of dogs were collected at 10 public squares. After that, these feces samples were transported to the Parasitology Laboratory in the UESC and they were analyzed by Mariano and Carvalho's method. Of the total analized samples, 56.3% show some parasitic evolutive form. Ancylostoma sp. was the most frequently (47.9%), followed by Strongyloides stercortalis (6.7%), Toxocara canis and Trichuris vulpis eggs (4.2%) each, Endolimax nana cysts (2.5%), and Giardia intestinalis and Entamoeba coli cysts (0.8%) each.","PeriodicalId":49617,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"93 1","pages":"206-209"},"PeriodicalIF":1.3,"publicationDate":"2008-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80270618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}