Synthetic Biology and Engineering最新文献

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Rapid Production of High-Titer d-Mannitol and Gluconate Catalyzed by a Combination of Whole-Cell and an Enzyme at High Temperatures 全细胞和酶在高温下催化快速生产高滴度d-甘露醇和葡萄糖酸盐

Synthetic Biology and Engineering Pub Date : 2025-07-01 DOI: 10.70322/sbe.2025.10011

Xinyue Liu, Pingping Han, Y.‐H. Percival Zhang

{"title":"Rapid Production of High-Titer d-Mannitol and Gluconate Catalyzed by a Combination of Whole-Cell and an Enzyme at High Temperatures","authors":"Xinyue Liu, Pingping Han, Y.‐H. Percival Zhang","doi":"10.70322/sbe.2025.10011","DOIUrl":"https://doi.org/10.70322/sbe.2025.10011","url":null,"abstract":"d-Mannitol and d-gluconate are value-added biobased chemicals with diverse applications in food, medical, and chemical industries. d-Mannitol can be hydrogenated from hexoses (e.g., d-fructose) catalyzed by microbial fermentation, whole-cell biocatalysis, and purified-enzyme cascade biocatalysis. Here we designed a cell–enzyme system comprised of the whole cells co-expressing both hyperthermophilic mannitol dehydrogenase (MDH) and glucose dehydrogenase (GDH) as well as a hyperthermophilic xylose isomerase (XI). The whole cells have its inherent NAD enabled to implement NAD-self sufficient coupled redox reactions without externally-added NAD and aeration. Four cases of whole cells co-expressed MDH and GDH in E. coli BL21(DE3) were compared and optimized by expressing two genes separately or in tandem and changing gene alignment. Also, two-step biotransformation was developed to convert 300 g/L glucose to 129 g/L mannitol and 161 g/L gluconate in a pH-controlled bioreactor at 70 °C. This cell–enzyme system had a high volumetric productivity (10.7 g/L/h mannitol and 13.4 g/L/h gluconate) and a high product yield (91.7%). This study implied that using hyperthermophilic enzymes and cell–enzyme system could open great opportunities for industrial biomanufacturing.","PeriodicalId":494707,"journal":{"name":"Synthetic Biology and Engineering","volume":"1 1","pages":"10011-10011"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciepublish.com/index/article/view_full_text/id/611","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Quorum Sensing Systems Engineering for Enhanced iso-Butylamine Production in Escherichia coli 提高大肠杆菌异丁胺产量的群体感应系统工程

Synthetic Biology and Engineering Pub Date : 2025-01-01 DOI: 10.70322/sbe.2025.10008

Mingxiong Liu, Yang Li, P. H. Yu, Hongxin Fu, Jufang Wang

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引用次数: 1