Acta odontológica latinoamericana最新文献

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In vitro cytotoxicity of resin cement and its influence on the expression of antioxidant genes 树脂水泥的体外细胞毒性及其对抗氧化基因表达的影响

Acta odontológica latinoamericana Pub Date : 2023-08-28 DOI: 10.54589/aol.36/2/120

Priscila FA Moralez, Kamila R Kantovitz, Elizabeth F Martinez, Lucas N Teixeira, Ana PD Demasi

{"title":"In vitro cytotoxicity of resin cement and its influence on the expression of antioxidant genes","authors":"Priscila FA Moralez, Kamila R Kantovitz, Elizabeth F Martinez, Lucas N Teixeira, Ana PD Demasi","doi":"10.54589/aol.36/2/120","DOIUrl":"https://doi.org/10.54589/aol.36/2/120","url":null,"abstract":"Aim: This study evaluated cytotoxicity and antioxidant gene expression of resin cements on human gingival fibroblasts (hGF). Materials and Method: RelyX UltimateTM(RXU), VariolinkTMII(VLII), and RelyXU200TM(RXU200) resin cements were incubated with culture medium for 24 h to obtain eluates. Then, the eluates were applied over hGF to assess cell viability at 24 h, 48 h, and 72 h and antioxidant gene expression at 24 h. hGF cultures non-exposed to the eluates were used as Control. Data were submitted to ANOVA and Bonferroni tests (α≤0.05). Results: RXU and RXU200 reduced the number of viable cells in 24 h. Longer exposure to cement extracts caused cell death. Gene expression showed peroxiredoxin 1 (PRDX1) induction by all resin cement types, and superoxide dismutase 1 (SOD1) induction by RXU200 and VLII. Moreover, RXU200 induced not only PRDX1 and SOD1, but also glutathione peroxidase 1 (GPX1), catalase (CAT), and glutathione synthetase (GSS). Conclusions: All resin cements showed toxicity, and induced antioxidant genes in hGF. Antioxidant gene induction is at least partly associated with cytotoxicity of tested cements to oxidative stress experience.","PeriodicalId":486248,"journal":{"name":"Acta odontológica latinoamericana","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135134513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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