المجلة المصرية للبيولوجيا التجريبية. علم النبات最新文献

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Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study 白桦茸提取物对金黄色葡萄球菌和鼠伤寒沙门菌的体外抑菌活性研究

المجلة المصرية للبيولوجيا التجريبية. علم النبات Pub Date : 2023-01-01 DOI: 10.5455/egyjebb.20230921012834

Majdah Tuwaijri, Sayed Souad

{"title":"Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study","authors":"Majdah Tuwaijri, Sayed Souad","doi":"10.5455/egyjebb.20230921012834","DOIUrl":"https://doi.org/10.5455/egyjebb.20230921012834","url":null,"abstract":"Inonotus obliquus (Chaga mushroom) is a herbal and edible mushroom widely distributed in different regions, especially temperate regions. Chaga parasites on the trunk of angiosperms and grows, forming an irregular black mass. The medical importance of Chaga has been reported from ancient cultures to data due to its activity against a wide range of diseases. In the present study, the ethyl acetate extract of the Chaga mushroom has been tested against two bacterial pathogens, Staphylococcus aureus ATCC25923 and Salmonella typhimurium ATCC14028. The antimicrobial activity of the extract showed high activity with inhibition zone diameters of 25 mm for S.aureus and 28 mm for S.typhimurium and minimum inhibitory concentration (MICs) of 3.125 µg/ml for both bacterial pathogens. GC-MS analysis of the extract revealed several compounds with seven major compounds; they are 3-pentanone, propyl butyrate, Ethyl propionate, Trimethylsilymethanol, Neopentyl glycol, n-butyl acetate, and 1-butanol-3-methyl acetate. The antibacterial mode of action of Chaga extract was also elucidated by studying the effect of the extract on cell membrane permeability and recoded high effect on bacterial cell membrane permeability, which was confirmed by transmission electron microscope (TEM) images that revealed leakage of bacterial cell content outside the cells and death.","PeriodicalId":485445,"journal":{"name":"المجلة المصرية للبيولوجيا التجريبية. علم النبات","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and Ultrastructure Studies on Human Sperms in cases of Unexplained Infertility Undergoing Intracytoplasmic Sperm Injection (ICSI) 不明原因不孕患者行胞浆内单精子注射(ICSI)的精子分子及超微结构研究

المجلة المصرية للبيولوجيا التجريبية. علم النبات Pub Date : 2023-01-01 DOI: 10.5455/egysebz.20230907033641

Azza Attia, Heba Nada, Yasser Orief, Ahmed Etman, Ahmed Abdelkareem

{"title":"Molecular and Ultrastructure Studies on Human Sperms in cases of Unexplained Infertility Undergoing Intracytoplasmic Sperm Injection (ICSI)","authors":"Azza Attia, Heba Nada, Yasser Orief, Ahmed Etman, Ahmed Abdelkareem","doi":"10.5455/egysebz.20230907033641","DOIUrl":"https://doi.org/10.5455/egysebz.20230907033641","url":null,"abstract":"Infertility is a global health problem, affects 25% of couples. It is described as the incapacity of a couple to conceive a child after 12 months of continued sexual intercourse. Unexplained infertility is described and diagnosed as the patients’ semen analysis is within normal values and the patient’ partner examinations revealed no causes of infertility. The study aimed to find out the causes of unexplained infertility in men using ICSI. This was done through evaluation of the oxidative stress (OS) parameters, semen, molecular analysis (Caspase-3 activity and DNA integrity), detecting the immunological antisperm antibody (ASA) in semen samples; in addition to the ultrastructural evaluations of spermatozoa using TEM examination. Regarding to OS analysis, the results showed significant increases in the concentrations of MDA in the seminal fluids of patients, while SOD and GSH activities revealed significant decreases, comparing to the control value. Catalase contents showed no significant differences. Cspase-3, DNA integrity (genetic diversity) as well as Antisperm antibody (ASA) analysis revealed significant increases in samples of unexplained ferirtility patients, compairing to the control. The electron micrographs showed the appearance of head and tail abnormalities, where there were marked deformities in size of head region, loss of chromatin contents and an appearance of intranuclear cytoplasmic retention (INCR). The axoneme showed incomplete number of microtubules and marked loss of the central axonemal doublets; in addition to the dysplasia and disorganization of the mitochondrial and the the fibrous sheath.","PeriodicalId":485445,"journal":{"name":"المجلة المصرية للبيولوجيا التجريبية. علم النبات","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135651863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0