Ecotoxicology and Environmental Contamination最新文献

Impact of Exposure Duration on DNA Damage Scores in the Comet Assay Using Allium cepa L. and Lactuca sativa L. 彗星试验中暴露时间对薤白和乳草 DNA 损伤评分的影响

Ecotoxicology and Environmental Contamination Pub Date : 2024-07-23 DOI: 10.5132/eec.2024.01.04

L. Andrade-Vieira, Franceli Aparecida da Cruz, Graciele Lurde Silveira, Silvana Marcussi

{"title":"Impact of Exposure Duration on DNA Damage Scores in the Comet Assay Using Allium cepa L. and Lactuca sativa L.","authors":"L. Andrade-Vieira, Franceli Aparecida da Cruz, Graciele Lurde Silveira, Silvana Marcussi","doi":"10.5132/eec.2024.01.04","DOIUrl":"https://doi.org/10.5132/eec.2024.01.04","url":null,"abstract":"The single-cell gel electrophoresis (SCGE), or comet assay, is a simple method that uses microgel and electrophoresis to evaluate damage in the DNA of the cells. The first protocol adapted for plants was described in the 1990s, however, a universal standardized procedure is not available to date. This study aimed to evaluate the influence of exposure time in the comet assay in plant models Allium cepa L. and Lactuca sativa L. Root tips of both plant models were exposed to methyl methanesulfonate (MMS), a known genotoxicant, for 2 h to 36 h. The alkaline version of the comet assay for plant samples was performed, and the DNA damage was assessed. Arbitrary units (AU) were calculated and compared to the negative control. MMS induced DNA damage (P < 0.05) after 2, 6, 10, 14, 28, 32 and 36 h of exposure in A. cepa, and at 2, 8, 10, 14 and 36 h for L. sativa. The highest AU values for A. cepa were observed after 4 h of exposure to MMS and for L. sativa, after 24 h of exposure. The optimal exposure times considering both genotoxic and mutagenic risk were indicated.","PeriodicalId":479767,"journal":{"name":"Ecotoxicology and Environmental Contamination","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141813372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation Kinetics of Antifouling Biocides in Sediment During the Spiking Equilibrium Phase 添加平衡阶段沉积物中防污杀生物剂的降解动力学

Ecotoxicology and Environmental Contamination Pub Date : 2024-07-20 DOI: 10.5132/eec.2024.01.05

Bruno Galvão de Campos, F. Perina, F. Abreu, Gilberto Fillmann, D. M. D. S. Abessa

{"title":"Degradation Kinetics of Antifouling Biocides in Sediment During the Spiking Equilibrium Phase","authors":"Bruno Galvão de Campos, F. Perina, F. Abreu, Gilberto Fillmann, D. M. D. S. Abessa","doi":"10.5132/eec.2024.01.05","DOIUrl":"https://doi.org/10.5132/eec.2024.01.05","url":null,"abstract":"The new generation of antifouling biocides, as well as other emerging contaminants, has not yet been included in sediment quality guidelines or present standard protocols for sediment spiking. Most of these biocides have short half-lives in water, but little information is available regarding their degradation in sediments. Thus, there is a need to establish a reliable duration of the equilibrium phase for sediment spiking prior to sediment toxicity testing to determine the actual exposure concentrations during ecotoxicological tests. This study aimed to evaluate the degradation of DCOIT, Irgarol, Diuron, and Dichlofluanid during a spiking equilibrium phase of 24 h at three different time intervals (0, 6, and 24 h) and concentrations (10, 100, and 1000 ng g-1), by applying kinetic degradation models. The models presented a better fit for the 1000 ng g-1 treatments, in which the half-lives of DCOIT and Diuron were < 5 h, Dichlofluanid < 2 h, and Irgarol < 6h. Our results also indicate that, except for Dichlofluanid, the degradation rates of the other antifouling biocides were reduced dramatically after 6 h of equilibrium. Therefore, an equilibrium phase of 24 h (or greater than 6 h) was considered viable for sediment spiking. Our findings provide valuable information to guide future sediment toxicity tests using these compounds.","PeriodicalId":479767,"journal":{"name":"Ecotoxicology and Environmental Contamination","volume":"115 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insecticides Cypermethrin and Chlorpyrifos Exposure: Effects on Sperm Motility and Fertility in Cyprinus carpio (Cypriniformes, Cyprinidae) 接触杀虫剂氯氰菊酯和毒死蜱：对鲤形目鲤科鱼精子活力和生育力的影响

Ecotoxicology and Environmental Contamination Pub Date : 2024-07-19 DOI: 10.5132/eec.2024.01.03

Federico Argemi, Andrés Atilio Porta, D. Streit Jr, Rômulo Batista Rodrigues, J. Benato, F. L. Nostro

{"title":"Insecticides Cypermethrin and Chlorpyrifos Exposure: Effects on Sperm Motility and Fertility in Cyprinus carpio (Cypriniformes, Cyprinidae)","authors":"Federico Argemi, Andrés Atilio Porta, D. Streit Jr, Rômulo Batista Rodrigues, J. Benato, F. L. Nostro","doi":"10.5132/eec.2024.01.03","DOIUrl":"https://doi.org/10.5132/eec.2024.01.03","url":null,"abstract":"The agricultural production of the Argentine Pampa is based on intensive cultures, and the most widely used insecticides correspond to cypermethrin (pyrethroid) and chlorpyrifos (organophosphate), among others. Sperm motility and fertility assessments are essential topics for biology and population dynamics studies on fish species. In this study the alterations of these endpoints after exposure to commercial formulation of both insecticides were used to analyze the effects on common carp sperm. After the in vitro exposure at nominal concentrations of 0.25, 0.5, 1, and 2 μg/L of cypermethrin, a significant reduction in motility in 0.5 μg/L concentrations was observed respect control group. The same nominal concentrations were tested for chlorpyrifos, and the same significative effect was detected at 2.0 μg/L. Fertilization rate was significantly reduced at concentrations from 0.5 μg/L cypermethrin exposure, and from 1.0 μg/L in the case of chlorpyrifos. Eggs LC50 / 24 h (95% confidence limit) exposed to cypermethrin was 0.57 μg/L (0.36 - 0.88), and exposed to chlorpyrifos was 2.45 μg/L (1.13-5.29). However, is still debatable how these insecticides impact on fish reproduction, using this in vitro scenario we found a clear reduction in sperm motility and fertility.","PeriodicalId":479767,"journal":{"name":"Ecotoxicology and Environmental Contamination","volume":" 509","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141823506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time-dependent Biochemical Responses of the Zoanthid Zoanthus sp. Exposed to Polyvinyl Chloride Microplastics 暴露于聚氯乙烯微塑料中的藻类Zoanthus sp.随时间变化的生化反应

Ecotoxicology and Environmental Contamination Pub Date : 2024-07-19 DOI: 10.5132/eec.2024.01.02

Luis Felipe Albertoni, Giorgi Dal Pont, L. R. Souza-Bastos, A. Ostrensky, João Henrique Alliprandini da Costa, Helen Sadauskas-Henrique

引用次数: 0