Middle east journal of science最新文献

筛选
英文 中文
ASSESSING THE POTENTIAL PUBLIC HEALTH RISK OF Clostridium botulinum TOXIN GENES IN CANNED FOOD : A LABORATORY EXPERIENCE IN TÜRKİYE 评估罐头食品中肉毒梭菌毒素基因的潜在公共卫生风险:土耳其的实验室经验
Middle east journal of science Pub Date : 2024-06-05 DOI: 10.51477/mejs.1437831
Mehmet Yılmaz, A. Yiğin, Serap Kılıç Altun, M. E. Aydemir
{"title":"ASSESSING THE POTENTIAL PUBLIC HEALTH RISK OF Clostridium botulinum TOXIN GENES IN CANNED FOOD : A LABORATORY EXPERIENCE IN TÜRKİYE","authors":"Mehmet Yılmaz, A. Yiğin, Serap Kılıç Altun, M. E. Aydemir","doi":"10.51477/mejs.1437831","DOIUrl":"https://doi.org/10.51477/mejs.1437831","url":null,"abstract":"Due to the ability to synthesize a highly potent neurotoxin, Clostridium botulinum poses an important risk to food safety. Foodborne botulism is a neuroparalytic disease caused by the ingestion of neurotoxins produced by Clostridium botulinum. Botulism commonly occurs as a result of consuming canned foods. In this study, the aim was to investigate the presence of C. botulinum and detect the genes producing A, B, E, and F toxins in C. botulinum isolates in homemade and commercial canned foods using the rapid, sensitive, and reliable Real-Time PCR technique. A total of 81 canned samples were collected, including 51 commercial and 30 homemade cans. The canned samples were initially enriched in tryptone peptone glucose yeast (TPGY) medium. Subsequently, DNA isolation was performed using the foodproof® StarPrep Two Kit. After the isolation process, Real-Time PCR was conducted using the foodproof® Clostridium botulinum Detection LyoKit and 5’ Nuclease (Biotecon, R60240) kit. Only 2 samples tested positive among all the samples. Both positive samples were identified to contain neurotoxin type A. They were both commercial canned foods—one was a mixed vegetable garnish, and the other was roasted red pepper. Both positive samples were packaged in glass containers. Consequently, it was concluded that commercially available canned foods in Turkey could still pose a potential risk to public health due to the presence of C. botulinum. Therefore, it was recommended to perform quality control analyses through food inspections in companies involved in canned food production and sales.","PeriodicalId":476738,"journal":{"name":"Middle east journal of science","volume":"18 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141385201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信