{"title":"FATTY-ACID COMPOSITION OF IMPORTED NUTS SOLD IN THE MARKETS OF THE CITY OF ASTANA","authors":"Auteleyeva L. T., Maikanov B.S., Smagulova A. S.","doi":"10.51452/kazatuvc.2023.3(003).1505","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1505","url":null,"abstract":"Nuts are high in protein, unsaturated fats, fiber, vitamins, and minerals. 70-80% of the fatty acids present in nuts and seeds are essential fatty acids, which are components of the plasma membrane and contain lecithin, a lipoprotein found in brain cells. The findings of investigations on the fatty acid makeup of several types of imported nuts in Astana's marketplaces are presented in this article. In this study, using the HPLC method, 17 samples of nuts imported from Uzbekistan, Iran, China have been analyzed. The experimental part was carried out in an accredited testing laboratory of Nutritest LLP, Almaty. As a result of the research, nut samples taken from the Shapagat, Alem, Sharyn, Astanalyk Bazaar, Eurasia-1 markets exceeded the norms of fatty acid composition from 1 to 20% in all samples. In addition, differences were found in the fatty acid profile of nuts of different types and origin. Peanuts from China, for example, have the highest quantities of palmitic acid, whereas cashews from Iran have the highest levels of stearic acid. Almonds also have the highest oleic acid values, while walnuts have the highest linoleic acid levels. These results may be useful in understanding the differences between nuts and choosing the most appropriate options for consumption based on individual needs and nutritional recommendations","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jakupov I. Т., Abultdinova A. B., Mamytbekova G. К., Zharkimbaeva Z. Z., Yeszhanova G. T., Zabrodin A. G.
{"title":"THE EFFECTIVENESS OF UTERINE DISEASE DIAGNOSIS METHODS IN COWS DEPENDING ON POSTPARTUM DAYS","authors":"Jakupov I. Т., Abultdinova A. B., Mamytbekova G. К., Zharkimbaeva Z. Z., Yeszhanova G. T., Zabrodin A. G.","doi":"10.51452/kazatuvc.2023.3(003).1520","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1520","url":null,"abstract":"The article presents the results of the effectiveness of methods for diagnosing the uterus in cows on different days after calving. In the postpartum period in cows under conditions of weakened immunity and dystocia, inflammatory processes in the uterus can develop, most commonly manifesting in clinical and subclinical forms of endometritis. Our research shows that the rectal diagnostic method is impact in the first 30 day in milk (DIM), particularly when clinical signs are evident. In 60 DIM, the vaginal method demonstrated about 58.8% effectiveness, by the using on \"Metrasta-tum\" device determine the degree of uterine involution and assess the obtained dis-charges. After 61 DIM the probability of detecting endometritis by laboratory methods increased from 6.3 to 25%. In this study, laboratory methods such as the Nagornyi-Kalinovsky test (NKT) and the Whiteside method (WM) were employed. Subclinical forms of inflammatory processes in the uterus in the absence of obvious visible signs imply the use of laboratory methods based on identifying inflammatory markers. The definition of effective and useful methods is an urgent task and requires additional re-search.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Almagambetov K. Kh., Sarmurzina Z. S., Chshelkina A. A., Zhakenov D. Sh., Sadykova B. J., Bisenova G. N., Shaikhin S. M.
{"title":"VIABILITY AND ANTAGONISM OF CRYOPRESERVED LACTIC ACID BACTERIA","authors":"Almagambetov K. Kh., Sarmurzina Z. S., Chshelkina A. A., Zhakenov D. Sh., Sadykova B. J., Bisenova G. N., Shaikhin S. M.","doi":"10.51452/kazatuvc.2023.3(003).1500","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1500","url":null,"abstract":"Preserving and advancing bioresources involving industrial microorganisms is of paramount importance for every nation. However, long-term storage of these strains often leads to diminished viability and biological activity. Thus, it is crucial to investigate the properties of cryopreserved strains stored at -80°C in a 10% glycerol solution within low-temperature refrigerators. This study aimed to comparatively analyze the viability of 129 lactic acid bacteria strains, including Lactobacillus sp., Lactococcus sp., and Pediococcus sp., cryopreserved from 2006 to 2020. Among them, 93 Lactobacillus sp. strains were categorized into three groups based on storage dates (2006-2007, 2013-2014, and 2017-2020). Viability titers were determined using the standard serial dilution method, counting microorganisms in CFU/ml. Regardless of the storage duration or species affiliation, the study identified lactic acid bacteria strains exhibiting both high (107 - 109 CFU/ml) and low (104-106 CFU/ml) viability titers. Additionally, the antagonistic activity of 33 Lactobacillus sp. strains was investigated using the delayed antagonism method, subdividing them into 17 strains with sufficiently high viability titers and 16 strains with low viability titers. The results revealed that 20% of strains with high viability titers and 27% with low viability titers exhibited relatively high antagonistic activity (with a zone of inhibition ranging from 10 to 18 mm). In both groups, strains with low antagonistic activity (with a zone of inhibition measuring 5-9 mm), particularly against Gram-positive and Gram-negative bacterial test-cultures, predominated. Significantly, 51% of lactobacilli strains demonstrated pronounced antagonism against Candida albicans ATCC- 885-653 test-culture. These findings underscore the practical importance of the study, emphasizing the necessity to analyze and select optimal concentrations of intracellular or extracellular cryopreservatives and to determine the initial viabilitytiter when storing strains for cryopreservation. Tailoring cryopreservation solutions to each strain can enhance the preservation of their original properties, ultimately improving overall preservation quality.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alieva G. K., Rychshanova R. M., Murzakayeva G. К., Mendybayeva A. M.
{"title":"ANTIBIOTIC RESISTANCE OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM ANIMALS AND BIRDS IN THE TERRITORY OF KOSTANAY REGION","authors":"Alieva G. K., Rychshanova R. M., Murzakayeva G. К., Mendybayeva A. M.","doi":"10.51452/kazatuvc.2023.3(003).1508","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1508","url":null,"abstract":"This article reveals the features of the resistance of Staphylococcus аureus strains isolated from animals, birds and animal products in the territory of Kostanay region. Staphylococci can infect any tissue or organ of an animal, causing more than 100 different diseases. According to literary sources, more than a dozen species of non-pathogenic or conditionally pathogenic staphylococci are isolated from many agricultural and domestic animals and birds. For the first time in the Kostanay region, the analysis of antibiotic resistance of staphylococcus strains isolated in livestock farms and animal products was carried out.The analysis of antibiotic resistance of staphylococcus strains isolated in livestock farms of Kostanay region and animal products was carried out. The study showed that the largest number of isolates of Staphylococcus аureus showed high resistance to beta-lactam antibiotics by an average of 55.3%, fluoroquinolones by 47.1% and tetracyclines by 45.6%.Of the 69 studied isolates of Staphylococcus аureus, antibiotic resistance was shown by 43 (62.3%) isolates, 26 (37.7%) isolates were sensitive to all groups of AMD.It was found that the largest number of isolates is 62.3% in the group of beta-lactams resistant to various antibiotics, the least resistant strains are 7.9% in the group of aminoglycosides and 20.3% in the group of sulfonamides.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. S. Akibekov, A. М. Gajimuradova, A. Kh. Zhumalin, S. M. Duisenov, N. G. Gubaidullin, N. A. Askarova
{"title":"SERINE PROTEASE OF TRICHINELLA SPIRALIS AND ITS POSSIBLE APPLICATION IN THE EARLY DIAGNOSIS OF TRICHINELLOSIS IN ANIMALS","authors":"O. S. Akibekov, A. М. Gajimuradova, A. Kh. Zhumalin, S. M. Duisenov, N. G. Gubaidullin, N. A. Askarova","doi":"10.51452/kazatuvc.2023.3(003).1490","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1490","url":null,"abstract":"Serine proteases of parasites play a significant role in the infection of a host organism. This is especially reflected in the process of immunosuppression, in particular, during IgG hydrolysis. The importance of the performed functionensures their presence at all stages of development, which makes them key targets for early diagnosis of trichinellosis. In this study, we demonstrated that serineprotease transcripts are detected in 83% of cases when mice are infected with BALB/c on days 7 and 14 after infection and are identified by molecular methods.Two groups of mice were infected with different doses of larvae (100 and 250 larvae), it was shown that the dosage did not affect the effectiveness of serine protease detection in mice, which would provide a better diagnostic effect. The transcripts presence confirms the possibility of using serine protease as a protein for the diagnosis of trichinellosis in animals and humans.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. K. Abdrakhmanov, Y. Y. Mukhanbetkaliyev, А. А. Mukhanbetkaliyeva, K. K. Beisembayev, A. A. Bayniyazov, S. Narbayev, K. K. Yessenbayev
{"title":"EPIDEMIOLOGICAL MONITORING THE PESTE DES PETITS RUMINANTS IN THE REPUBLIC OF KAZAKHSTAN","authors":"S. K. Abdrakhmanov, Y. Y. Mukhanbetkaliyev, А. А. Mukhanbetkaliyeva, K. K. Beisembayev, A. A. Bayniyazov, S. Narbayev, K. K. Yessenbayev","doi":"10.51452/kazatuvc.2023.3(003).1501","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1501","url":null,"abstract":"Peste des petits ruminants (PPR) is a highly contagious viral disease of sheep and goats, as well as wild small ruminants, occurring mainly in acute or subacute clinical forms. PPR causes great economic damage to the small-scale sheep and goat farming and as the whole states. The article presents epidemiological monitoring and analysis of preventive measures carried out in Kazakhstan against PPR. The country’s territory is conditionallydivided into two safe zones (territories with and without vaccination). Specific PPR prevention is carried out in a safe zone with vaccination (buffer zone), which includes five regions and a city of republican significance (Almaty, Zhambyl, Zhetysu, Kyzylorda, Turkestan regions and Shymkent). In 2018-2022 from 3225570 to 6733974 sheep and goats were vaccinated in buffer zones annually. At the same time, vaccination coverage from thetotal number of susceptible animals was 15.2-36.8%.Planned monitoring diagnostic studies have confirmed the epidemiological well-being of the country in terms of PPR. Over the past 5 years, 86,830 serological and 482 molecular genetic studies have been conducted, with negative results in all cases. Serological monitoring of «risk zones of possible infection» for the presence of antibodies to the PPR virus in susceptible pets also confirmed the absence of infection in the studied territories.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"157 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135427305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glimåker A., Getya A., Kravchenko O., Zamaratskaia G.
{"title":"WASTE AND BY-PRODUCTS FROM THE MEAT INDUSTRY AS A SOURCE OF BIOACTIVE COMPOUNDS","authors":"Glimåker A., Getya A., Kravchenko O., Zamaratskaia G.","doi":"10.51452/kazatuvc.2023.3(003).1507","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1507","url":null,"abstract":"The aim of this review was to summarize existing knowledge on bioactive peptides from the waste and by-products from meat industry and identify future directions of research. Reducing food waste and transforming it into food and feed is a step toward the achievement of economic development while reducing adverse effects on the environment. Meat production and consumption have increased in recent years. Above 20% of meat is wasted in Europe throughout the different steps in the production chain. Meat waste and by-products are rich sources of proteins, bioactive compounds, essential amino acids, vitamins and minerals, and have a potential to be further used for dietary and non-dietary purposes. Bioactive peptides often have health-promoting effects such as antioxidant, anti-hypertensive, anti-inflammatory, antimicrobial and antitumor activities when ingested and absorbed by human beings. Some bioactive peptides were shown to reduce the risk of development of cancer, diabetes and cardiovascular disease, which are among the most common diseases currently. Several methods to isolate bioactive peptides from meat waste and by-products were developed. Enzymatic hydrolysis can generate hydrolyzates and certain bioactive peptides from larger proteins, and the hydrolyzates can be used as dietary supplements or as an additive to increase the protein content of food. Bioactive peptides can be isolated from the hydrolyzates and used as functional food components, dietary supplements or medicines. We concluded that wastes and by-products from the meat industry present an opportunity for the generation of health-promoting bioactive compounds which can be successfully used in food and other relevant industries. However, existing research mainly is mainly focused on theinfluence of individual bioactive peptides on certain health parameters and is mainly performed using in vitro methods. Thus, future research should be directed to assess the long-term effects of bioactive compounds as a component of the whole diet.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. S. Tursynbayev, N.N. Akhmetsadykov, Zh. M. Batanova, Khussainov D. M., Krykbayev Y. A.
{"title":"DEVELOPMENT OF THE TECHNOLOGY OF DEEP CULTIVATION OF A TRANSFORMED B. SUBTILIS STRAIN","authors":"N. S. Tursynbayev, N.N. Akhmetsadykov, Zh. M. Batanova, Khussainov D. M., Krykbayev Y. A.","doi":"10.51452/kazatuvc.2023.3(003).1494","DOIUrl":"https://doi.org/10.51452/kazatuvc.2023.3(003).1494","url":null,"abstract":"Deep cultivation of recombinant cultures in bioreactors makes it possible to accumulate a large number of specific antigens used in the production of immunobiological preparations and diagnostic test systems in a short period of time, taking into account stimulating and limiting growth factors. The main purpose of the research was to determine the influence of the factors of seed concentration, pH level and the level of dissolved oxygen on deep cultivation, with the control of accumulation kinetics by turbidimetry. During the research, it was found that the most optimal inoculation concentration is 1 million cells/cm3 with a maximum final concentration of B. subtilis 4x106 cells/cm3, the most optimal pH level is a dynamic mode from 8.0 to 6.0 with a maximum final concentration of 5x106 cells/cm3, the most optimal level of dissolved oxygen is stationary mode with a minimum oxygen level of 25%, with a maximum final concentration of 4x106 cells/cm3.Bacteriological, technological and biochemical methods were used in the work to develop the modes of deep cultivation of the transformed B. subtilis strain with control of pH parameters and the level of dissolved oxygen.The scientific significance of the research is based on the development of deep cultivation, with an assessment of external growth factors and their influence on the kinetics of accumulation of the transformed B. subtilis strain, which will provide effective conditions for the growth and production of microorganisms. The practical significance of the research is based on the possibility of optimizing the biotechnological stages of the cultivation of the transformed B. subtilis strain, which will positively affect the efficiency of submerged cultivation as the main method for the accumulation of specific antigens.","PeriodicalId":476095,"journal":{"name":"HERALD OF SCIENCE OF S SEIFULLIN KAZAKH AGRO TECHNICAL RESEARCH UNIVERSITY Veterinary sciences","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135428577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}