Journal of Agricultural Chemistry and Biotechnology (Print)最新文献

Factors Affecting Biodegradation of the Insecticides by Cyanobacterial Strains, Isolated from Soil Contaminated with Pesticides 农药污染土壤中蓝藻菌株生物降解杀虫剂的影响因素

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-11-08 DOI: 10.21608/jacb.2023.242992.1069

Aida Afify, F. Hauka, H. El- Zawawy, A. Abou Elatta

{"title":"Factors Affecting Biodegradation of the Insecticides by Cyanobacterial Strains, Isolated from Soil Contaminated with Pesticides","authors":"Aida Afify, F. Hauka, H. El- Zawawy, A. Abou Elatta","doi":"10.21608/jacb.2023.242992.1069","DOIUrl":"https://doi.org/10.21608/jacb.2023.242992.1069","url":null,"abstract":"This research aim to study the effect of some factors (concentration of insecticide, temperature and pH levels) affecting biodegradation of insecticides by cyanobacteria. The superior isolates out of twelve cyanobacterial isolates, which isolated from soil contaminated with insecticides were Nostoc muscorum and Anabaena oryzae . Results indicated that an increase in dry weight and N 2 fixation was recorded with increasing chlorpyrifos concentration from 0 up to150 ppm. These results were noted with Anabaena oryzae . On the other hand, there was an increase in the average of dry weight and N 2 fixation with increasing the concentration of carbofuran from zero up to 80 ppm. The highest nitrogen content was recorded in Nostoc muscorum. The biodegradation of the pesticide (chlorpyrifos and carbofuran in liquid cultures) by cyanobacterial isolates was evidenced by complete discoloration of cyanobacterial growth at 12 days of incubation. The fixed nitrogen and dry weight of Anabaena oryza e and Nostoc muscorum grown at different concentrations of chlorpyrifos (0, 50, 100, 150 and 200) and different temperature degrees (20,25,30 and 35°C) as well as different pH levels (5, 6, 7 and 8) were determined. Results indicated that the maximum uptake of chlorpyrifos by Anabaena oryzae and Nostoc muscorum was recorded at 30°C and pH 7.0. Moreover, the maximum removal of carbofuran by Anabaena oryza e and Nostoc muscorum at different concentrations (0, 40, 80 and 120) was recorded at 25°C and pH 7.0","PeriodicalId":475818,"journal":{"name":"Journal of Agricultural Chemistry and Biotechnology (Print)","volume":"57 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135431440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Bacterial Strains for Bio-Hydrogen Production from Domestic Wastewater in Microbial Electrolysis Cells 微生物电解池中生活废水生物制氢菌株的评价

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-10-17 DOI: 10.21608/jacb.2023.238316.1067

Aida Afify, A. Abd El Gwad, N. Abd-Elrahman

引用次数: 0

Molecular and Genotoxic Effects of Sildenafil Citrate and Tramadol on Testis and Sperm Quality in Rat Males: Role of Apoptosis 枸橼酸西地那非和曲马多对雄性大鼠睾丸和精子质量的分子和基因毒性作用:凋亡的作用

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-09-17 DOI: 10.21608/jacb.2023.228192.1063

E. A. Eltohamy, A. M. Hassan, Hoda Elgarhy, ِA. serag

引用次数: 0

Identification of Salinity Tolerant Gene in Six Wheat Genotypes (Triticum aestivum) Via SSR and Sequencing Technique 6个小麦基因型(Triticum aestivum)耐盐基因的SSR和测序鉴定

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-09-17 DOI: 10.21608/jacb.2023.227447.1062

S. Demais,, M. Abd EL Sabour, M. Bekhit, K. Gad, T. Salim

引用次数: 0

The Rule of Salinity Stress in Activation of Retrotransposition Rate in some Bread Wheat Genotypes. 盐胁迫对部分面包小麦基因型逆转录转位激活的影响规律。

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-09-17 DOI: 10.21608/jacb.2023.228750.1064

S. Demais,, M. Abd EL Sabour, M. bakhit, K. Gad, T. Salim

{"title":"The Rule of Salinity Stress in Activation of Retrotransposition Rate in some Bread Wheat Genotypes.","authors":"S. Demais,, M. Abd EL Sabour, M. bakhit, K. Gad, T. Salim","doi":"10.21608/jacb.2023.228750.1064","DOIUrl":"https://doi.org/10.21608/jacb.2023.228750.1064","url":null,"abstract":"The genomes of the six wheat genotypes under study; Masr1, Shandaweel1, Line1. Line2, Giza171 and Sakha94 were extracted. Ten IRAP primers were applied for detecting the rule of salinity stress in activation of retrotransposition rate in the studied bread wheat genotypes. The wheat genotypes were irrigated with 100 mM, 150 mM and 200 mM of NaCl or distilled water for control. IRAP technique developed all the ten markers in the wheat under the different levels of salinity. IRAP-2175 primer showed a number of new bands with molecular weights ranged from 400 bp to 550 bp giving a new retrotransposition locations due to the treatment with different concentrations of salinity. The second primer (IRAP-2198) revealed that bands with molecular sizes ranged from 470 bp to 1160 bp obtained a new retrotransposition appearance. The third IRAP marker was revealed by the IRAP-2197 primer with molecular sizes of 215 bp to 342 bp under all the three levels of salinity. The results revealed different patterns between control and treatments and the high levels of salinity led to new retrotransposition. This study revealed that PCR technique; like IRAP can reflect the activation of retrotransposition due to high salt levels. The obtained results were encouraging and it is better to use this technique due to their advantage; easy, fast, cheap and effectiveness.","PeriodicalId":475818,"journal":{"name":"Journal of Agricultural Chemistry and Biotechnology (Print)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135259641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L. 化学诱变剂对葱根尖细胞的细胞毒作用。

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-09-10 DOI: 10.21608/jacb.2023.219711.1057

T. Salim, M. Bekhit, Fathia Soudy

{"title":"Cytotoxic Effects of Chemical Mutagens in Root Tip Cells of Allium cepa L.","authors":"T. Salim, M. Bekhit, Fathia Soudy","doi":"10.21608/jacb.2023.219711.1057","DOIUrl":"https://doi.org/10.21608/jacb.2023.219711.1057","url":null,"abstract":"One of the excellent plant models for identifying various chromosomal abnormalities is Allium cepa L. In order to create genetic diversity, chemical mutagens such as; Ethyl methanesulfonate (EMS), Ethidium bromide (EtBr) and Sodium azide (SA) were used to mutagenize the plant's germplasm. The present study was carried out to determine how these chemicals affected the Mitotic Index (MI) and phase, chromosomal abnormalities, and frequency of micronuclei in the root tip cells of A. cepa Mutagens, in general, alter the genetic structure of the organisms, which is reflected in changes to a variety of features. Further, the genome's sensitivity was discovered through cytological examination when the three mutagens were used. The chromosomal entity was impacted by a variety of clastogenic and nonclastogenic alterations, as well as mitodepressive effects. The increase in dose/concentration of all the mutagens was shown to increase the frequency of both mitotic aberrant cells and mitotic chromosomal abnormalities in the treated root tip cells. Both chemical mutagens were shown to be less effective than EtBr at disrupting mitotic cell activity and primarily causing clastogenic chromosomal abnormalities. Numerous chromosomal aberrations, including stickiness and clumping of chromosomes with varying frequencies, fragments, laggards, bridges, precocious movements, micronuclei formation at telophases, and multipolar anaphases and telophases, were reported to be present in the mitotic aberrant cells. Anaphases, telophases, disorganised metaphases, desynchronized metaphases, and persisting nucleoli during","PeriodicalId":475818,"journal":{"name":"Journal of Agricultural Chemistry and Biotechnology (Print)","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136073564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial and Chemical Evaluation of Drinking Water Quality at El- Gharbia Governorate, Egypt 埃及加尔比亚省饮用水水质的细菌和化学评价

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-09-01 DOI: 10.21608/jacb.2023.230824.1066

Aida Afify, A. AbdAllah

{"title":"Bacterial and Chemical Evaluation of Drinking Water Quality at El- Gharbia Governorate, Egypt","authors":"Aida Afify, A. AbdAllah","doi":"10.21608/jacb.2023.230824.1066","DOIUrl":"https://doi.org/10.21608/jacb.2023.230824.1066","url":null,"abstract":"This study was conducted at El-Gharbia Governorate to evaluate the quality of water for domestic purposes. The samples of water were collected from different sites during 2012 /2013 in order to determine and identify the bacterial isolates. In addition, certain physical and chemical parameters like temperature, pH, turbidity, electrical conductivity, total of some chemicals (hardness, partial hardness, calcium, magnesium, calcium hardness, magnesium hardness, SO 4 , silica, iron, manganese, aluminum, phosphoras, ammonia), Nitrite, Nitrate and Chlorides were determined to evaluate quality of Nile, ground and tap water at five sites namely: Tanta, Elmehala-Elkobra, Mehalt Abo Ali, Samanod and Mehalt-Roh. In a previous study at 2016 about 60 bacterial isolates were detected on selective media after isolation from the three sources of drinking water. These bacterial isolates were belonging to genera: Staphylococcus, Aeromonas, Proteus, Serratia and Pantoea. In this study, the Vitek Microbial system was used for identified the bacterial genera speices as: Staphylococcus aureus, Aeromonas hydrophila, Proteus mirabilis, Serratia plymythica and Pantoea agglomerans . The physical and chemical analyses showed that water qualities of three water sources are convenient for both aquatic life and domestic purposes as the normal range within a River Nile and tap water but in ground water are different.","PeriodicalId":475818,"journal":{"name":"Journal of Agricultural Chemistry and Biotechnology (Print)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135690981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbofuran Relevance and their Cyanobacterial Degradation in Rice Fields 稻田中呋喃的相关性及其蓝藻降解

Journal of Agricultural Chemistry and Biotechnology (Print) Pub Date : 2023-08-01 DOI: 10.21608/jacb.2023.227084.1061

Aida Afify, F. Hauka, H. El- Zawawy, A. Abou Elatta

{"title":"Carbofuran Relevance and their Cyanobacterial Degradation in Rice Fields","authors":"Aida Afify, F. Hauka, H. El- Zawawy, A. Abou Elatta","doi":"10.21608/jacb.2023.227084.1061","DOIUrl":"https://doi.org/10.21608/jacb.2023.227084.1061","url":null,"abstract":"Carbofuran is widely used for controlling pests on rice plant. These pesticide pollute the environment. Cyanobacteria can be used to get rid of pesticides such as carbofuran able to remove from agricultural fields. Therefore, the main objective of this research is to assess the biodegradation of the carbofuran by Anabaena oryzae and Nostoc muscorum these are cyanobacterial strains in the rice fields which polluted by this insecticide. For determination of biodegradation carbofuran, nitrogen fixation rate, dry weight formation, total count of cyanobacteria, absorbance measurements by spectrophotometer in addition gas chromatography–mass spectrometry (GC-MS) used to perform carbofuran, residues. The data showed the ability of cyanobacteria to degrade all components of carbofuran by cyanobacteria and the mixture of Nostoc muscorum and Anabaena oryzae had improved in dry weight and increased the nitrogen fixation in environment. Furthermore, the growth and yield for rice plant after inoculation with the mixture treatment from cyanobacterial strains were increased compared to the control. In addition, it highlights the need for cyanobacteria application in the contaminated area is a better candidate for biological decomposition of insecticides","PeriodicalId":475818,"journal":{"name":"Journal of Agricultural Chemistry and Biotechnology (Print)","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134997316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1