Jentashapir Journal of Cellular and Molecular Biology最新文献

{"title":"Analysis of Factors Affecting Fetal Fraction in Cell-Free DNA Test","authors":"Behdad Amirgholami, Sara Masihi, Reza Samie, Hamid Galehdari, Gholam Reza Shariati","doi":"10.5812/jjcmb-145025","DOIUrl":"https://doi.org/10.5812/jjcmb-145025","url":null,"abstract":"Background: Noninvasive prenatal testing (NIPT) serves as a screening method to assess the risk of chromosomal abnormalities in the fetus, including trisomy 18, 21, and 13. The reliability and success of this test are influenced by the proportion of circulating cell-free DNA obtained from the feto-placental unit, known as the fetal fraction (FF). Objectives: Our study aims to investigate the fetal and maternal factors affecting FF. Methods: Our research involved 1 150 patients referred for NIPT due to various reasons such as maternal age over 35, high-risk screening tests in the first or second trimester, history of trisomy in previous pregnancies, or patient request. Patients completed a questionnaire providing variables including maternal and fetal age, body mass index (BMI), smoking status, multiple pregnancies, and medication use such as Heparin or enoxaparin. Noninvasive prenatal testing was conducted on blood samples using Ion proton technology by Premaitha of the UK. The results, including fetal sex, trisomy risk, fetal fraction, and abnormal sex chromosomes, were analyzed using IBM SPSS 27 to assess the relationship between FF percentage and other variables. Results: The study included 1150 NIPT cases, with maternal ages ranging from 13 to 48 years, BMI from 15 to 70, and gestational age from 10 to 27 weeks. Among these, 96.4% were singleton pregnancies and 3.6% were twin pregnancies. Spontaneous pregnancies accounted for 88.9%, while 9.2% were IVF and 1.2% were IUI. Fetal sex distribution was 45.9% female and 54.1% male. FF ranged from 4% to 20.7%, with a mean FF of 11.07. No significant correlation was found between maternal age, fetal age, maternal BMI, trisomies, and FF. Conclusions: We can see that maternal age has no significant correlation with the fetal fraction. Although fetal age increases, the fetal fraction does too, but this increase was not significant. Body mass index, the route of pregnancy, and the sex of the fetus had no effect on FF. Despite NIPS being safer and yielding better results, other factors can still influence the outcomes of NIPS. Therefore, this test remains a screening tool, and clinical counseling should be conducted both before and after the test.","PeriodicalId":475807,"journal":{"name":"Jentashapir Journal of Cellular and Molecular Biology","volume":"141 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140223894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Effect of Lovastatin and Selegiline on the Differentiation of Bone Marrow Stromal Cells Into Neuron-Like Cells","authors":"Alireza Abdanipour, Behnam Karami, Mohammad Javad Fridoni, Momeneh Mohamadi, F. Fakheri","doi":"10.5812/jjcmb-141092","DOIUrl":"https://doi.org/10.5812/jjcmb-141092","url":null,"abstract":"Background: The effect of selegiline as an oxidase inhibitor on cell differentiation into neuron-like cells has been demonstrated by altering gene expression. Based on the results of studies on the role of statins in neurotrophin regulation, in this study, we examined the effect of lovastatin (HMG-CoA reductase inhibitor) on the differentiation of bone marrow mesenchymal cells (BMSCs) into neuron-like cells. Selegiline is an irreversible inhibitor of monoamine oxidase (MAO) type B. Since dopamine in the human brain is metabolized primarily by MAO-B, selegiline increases dopamine levels in the central nervous system. In addition to inhibiting MAO-B, selegiline also inhibits the uptake of dopamine and norepinephrine into presynaptic nerves and increases dopamine turnover. Methods: Bone marrow mesenchymal cells were collected from 28-day-old rats and cultured under standard conditions on the medium. The experimental groups in this study were as follows: BMSCs (control); BMSCs induced with 20 µM selegiline for 24 hours (experiment 1); BMSCs induced with 6 µM lovastatin for 24 hours (experiment 2); BMSCs were induced with 20 µM selegiline for 24 hours and 6 µM lovastatin for the next 24 hours (experiment 3). Real-time RT-PCR was performed to determine the mRNA levels of the nestin and NF-68 genes. Results: Real-time RT-PCR results showed that nestin and NF-68 mRNA levels were significantly increased in the co-treatment group (experiment 3) compared to the other experimental groups (P < 0.05). Conclusions: Based on the increased expression of nestin and NF-68 genes, the presence of lovastatin has a synergistic effect on neuronal differentiation and optimization of stem cell therapeutic approaches.","PeriodicalId":475807,"journal":{"name":"Jentashapir Journal of Cellular and Molecular Biology","volume":"53 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138995445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and Simulation of Docking Peptide Labeled with Technetium-99m (Tc-99m) with Human Epidermal Growth Factor Receptor 2 (HER2) in HER2-Positive Breast Cancer Cells","authors":"Mohammad mehdi Reyhani, Amir abbas Minaeefar, Fereshteh Dadfar, Kourosh Bamdad","doi":"10.5812/jjcmb-139817","DOIUrl":"https://doi.org/10.5812/jjcmb-139817","url":null,"abstract":"Background: Breast cancer is the first fatal disease in women and the second leading cause of death in humans, after heart disease. In approximately 20% of these patients, breast cancer cells have a higher-than-normal level of a protein called human epidermal growth factor receptor 2 (HER2) on their surface. These cancers, called HER2-positive, tend to grow and spread faster than other breast cancers. Objectives: This study aimed to evaluate the binding of the peptide LTVSPWY labeled with the HER2 receptor compared to the non-labeled peptide and simulate its docking. Methods: The LTVSPWY peptide was first labeled with the 99-radioactive technetium (Tc-99m) and optimized for molecular docking with the HER2 receptor in HyperChem software. Then, the HEX 6 software was used to check how the compounds were connected to the active site of the HER2. Finally, data was analyzed by HEX, LIGPLOT, and other programs. Results: During docking in HEX, the labeled peptide showed a good link with the HER2 protein receptor. The energy of this kind of transplantation was higher than the nonlabeled peptide bond with the HER2 protein receptor. In fact, this combination had the lowest level of connection energy with the receptor, which reflects the success of docking. Conclusions: In the analysis of the post-docking data, we observed the ligand was connected to the HER2 receptor from 5 major sites at different intervals, indicating a suitable bond to the receptor ligand.","PeriodicalId":475807,"journal":{"name":"Jentashapir Journal of Cellular and Molecular Biology","volume":"22 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138605767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppressing Effect of Human Wharton’s Jelly Mesenchymal Stem Cell Secretomes on Oxidative Stress Induced by Breast Cancer Cell Line SK-BR3","authors":"Fatemeh Ahmadpour, Afrooz Karimi, Mohammd mahdi Saadatmandfar, S. Karimi","doi":"10.5812/jjcmb-141019","DOIUrl":"https://doi.org/10.5812/jjcmb-141019","url":null,"abstract":"Background: The main reason for treatment failure and the primary cause of breast cancer deaths is metastasis. Cancer features, such as epithelial to mesenchymal transition (EMT), invasiveness, stemness, and ability to metastasize, are significantly influenced by oxidative stress. Objectives: The primary objective of this work was to evaluate the effects of human Wharton’s jelly mesenchymal stem cell secretomes (hWJMSCs-Se) on oxidant contents and development of the breast cancer SK-BR3 cell line and alterations in EMT markers genes after treatment. Methods: SK-BR3 cells received 48 hours of treatment with 10, 25, or 50 μg/mL hWJMSCs-Se. The MTT test and colony formation were used to evaluate the SK-BR3 cells’ viability and proliferation capability. By using annexin V/propidium iodide (PI) staining, apoptosis was determined. The messenger ribonucleic acid (mRNA) expression levels in genes associated with antioxidants were additionally assessed. Antioxidant enzyme activity was checked after SK-BR3 treatment with hWJMSCs-Se. Results: In the hWJMSCs-Se-treated SK-BR3 cells, colony counts, and viability percentages decreased significantly with time and concentration. The treated cells displayed considerably greater apoptotic indices when compared to the control. Catalase (CAT), superoxide dismutase (SOD) activities, and glutathione (GSH) content were significantly greater in the hWJMSCs-Se-exposed SK-BR3 cells. The Vimentin gene and N-cadherin gene were significantly elevated in the treated cells, and E-cadherin and β-catenin decreased conversely. Conclusions: The present study suggests that the use of hWJMSCs in the treatment of human epidermal growth factor receptor 2 (HER2)-positive malignancies provides an innovative solution for cancer therapy. As the oxidant level and EMT pathway decreased, breast cancer cell growth was significantly restricted, and mortality increased.","PeriodicalId":475807,"journal":{"name":"Jentashapir Journal of Cellular and Molecular Biology","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138607144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}