Organoid (Online)最新文献

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Optimization of Matrigel concentration for salivary gland organoid culture 唾液腺类器官培养基质浓度的优化
Organoid (Online) Pub Date : 2023-10-25 DOI: 10.51335/organoid.2023.3.e14
Eunhoo Choi, Sangho Roh
{"title":"Optimization of Matrigel concentration for salivary gland organoid culture","authors":"Eunhoo Choi, Sangho Roh","doi":"10.51335/organoid.2023.3.e14","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e14","url":null,"abstract":"Background: Research on salivary gland tissue is important, both for maintaining good oral health and for understanding the properties and functions of secretory cells with similar developmental mechanisms and functions. However, research on the pathogenesis of salivary gland disease and the development of therapeutic agents is very insufficient, and no studies have yet reported effective approaches to changes that occur according to the concentration of Matrigel, which is used to mimic the interactions between cells and the extracellular matrix, in three-dimensional organoid culture systems.Methods: We aimed to identify the most suitable concentration of Matrigel for analyzing the morphology and differentiation patterns of organoids prepared using salivary submandibular gland-derived cells after inducing differentiation with or without a specific chemical, Y-27632.Results: In both low and high Matrigel concentration conditions, morphological differences were found between the control group and the Y-27632 treatment group, and the budding structure was also significantly higher in the Y-27632 treatment group. However, more distinct patterns of differentiation appeared in the high-concentration condition.Conclusion: The concentration of Matrigel in the 3-dimensional organoid culture system had a significant effect on mimicking the actual in vivo environment.","PeriodicalId":471660,"journal":{"name":"Organoid (Online)","volume":"3 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135168924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural biopolymer-based hydrogels as designer matrices for organoid cultures 基于天然生物聚合物的水凝胶作为类器官培养的设计基质
Organoid (Online) Pub Date : 2023-09-25 DOI: 10.51335/organoid.2023.3.e13
Yun Kee Jo
{"title":"Natural biopolymer-based hydrogels as designer matrices for organoid cultures","authors":"Yun Kee Jo","doi":"10.51335/organoid.2023.3.e13","DOIUrl":"https://doi.org/10.51335/organoid.2023.3.e13","url":null,"abstract":"Matrigel, a mouse sarcoma-derived extract, is considered the gold standard for organoid cultures. However, it has several drawbacks, including inconsistent and ill-defined composition, varying quality between batches, and potential cancer-related health risks. These factors highlight the need to develop chemically defined alternatives to Matrigel. Natural biopolymers derived from living organisms have emerged as promising substitutes capable of creating chemically defined extracellular matrix (ECM)-mimicking materials to support organoids in a 3-dimensional (3D) environment. This article provides an overview of natural biopolymeric hydrogel-based bioengineering approaches for constructing 3D matrices resembling artificial ECM for organoid cultures. It discusses the latest developments in utilizing natural biopolymers to direct the growth, differentiation, and maturation of organoids, along with their translational applications in the fields of bioengineering and biomedicine. Additionally, the article offers perspectives on multidisciplinary research on natural biopolymer-based hydrogels for more practical applications as next-generation matrices for organoid cultures.","PeriodicalId":471660,"journal":{"name":"Organoid (Online)","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135771081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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