{"title":"Response of Some Agronomical and Physiological Traits of Dracocephalum kotschyi Boiss., under Irrigation and Dryland Farming System in Northern East of Iran","authors":"M. Shaabani, M. Azizi, A. Jafari, S. Mousavi","doi":"10.22092/JMPB.2020.122075","DOIUrl":"https://doi.org/10.22092/JMPB.2020.122075","url":null,"abstract":"The Dracocephalum kotschyi Boiss.is a wild-growing flowering plant belonging to the Lamiaceae family and has been used as a medicinal herb for rheumatoid diseases, headaches, congestion, stomach disorders, liver treatment. In order to study the effects of dryland farming system on some agronomical and physiological traits of D. kotschyi, two separate experiments were conducted in three locations of, Mashad (Golmakan station), Quchan (5km in the northeast of city) and Bojnord (Sisab station) under normal irrigation and dryland farming systems in 2018. The collected data were combined analysis over three locations and mean comparisons were made using Tukey method. The results showed significant effects of locations for all of the traits except carbohydrate contents. Effect of farming system was also significant for all traits. Farming system by location interaction effects were significant for aerial fresh and dry weigh, 1000 seeds weight, proline, DPPH, flavonoid, protein, chlorophyll a, carotenoid, nitrogen and potassium. Result showed the location of Quchan had significantly higher mean values for many of traits followed by Mashad. In comparisons between two method of cultivations, the lower and higher values of plant height (20.6 vs. 25.3 cm), main branch (13.1 vs. 14.9 per plant), leaf area (38.9 vs. 48.3 mm2), flower number (14.6 vs. 23.1 per plant), fresh weight (295 vs. 888 g/plant), dry weight (80 vs. 244 g/plant), 1000 seeds weight (0.27 vs. 0.35 g), Protein (9.74 vs. 11.06%), chlorophyll a (7.47 vs. 8.19 mg g-1FW), carotenoid (0.08 vs. 0.11 mg g-1FW), nitrogen (1.43 vs. 1.77%) and phosphorus (13.89 vs. 20.79 ppm) were obtained in dryland and irrigation farming, respectively. In contrast, the higher and lower values of internode length (4.11 vs. 3.71 cm), proline (1.44 vs. 0.77 mg g-1FW), DPPH (0.39 vs. 0.19 mg g-1FW), phenol (0.83 vs. 0.67 mg g-1FW), flavonoid (0.60 vs. 0.47 mg g-1FW), carbohydrate (8.2 vs. 6.2 mg g-1FW), and potassium (4.2 vs. 3.7%) were obtained in dryland farming system.","PeriodicalId":41224,"journal":{"name":"Journal of Medicinal Plants and By-products-JMPB","volume":"9 1","pages":"59-69"},"PeriodicalIF":0.6,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49104927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic Diversity Assessment Between Different Populations of Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. in Iran using RAPD, ISSR and R-ISSR Markers.","authors":"Mahdi Nikraad, S. M. H. Hejazi, M. Pezhmanmehr","doi":"10.22092/JMPB.2019.119385","DOIUrl":"https://doi.org/10.22092/JMPB.2019.119385","url":null,"abstract":"The present study was conducted to investigate genetic diversity between and within of six populations with different individual numbers of cultivated and non-cultivated provenances of Moringa peregrina (Forssk.) Fiori and Moringa oleifera Lam. using RAPD, ISSR and various combinations of RAPD and ISSR primers in one multiplex PCR (R-ISSR). 10 primers that produced clear and reproducible fragments after screening of 30 primers were selected for further analysis. A set of 10 primers generated 96 bands ranging in size from 150 to 1600 bp, corresponding to an average of 16 bands per primer and out of which 100 % were polymorphic among 26 individuals. The PIC values ranged from 0.16 to 0.31 and MI values ranged from 2.16 to 4.65 per primer. The primer R-ISSR (H876+A17) had the highest PIC (0.31) and MI (4.65) values. A maximum and minimum genetic similarity values were observed between populations (I and V) in M. oleifera (0.98) and populations (III and IV) in M. peregrina (0.52) respectively. The Gst value was 0.7, indicating that 61% of the genetic diversity resided within the populations. Clustering analysis using average algorithm based on Nei's unbiased genetic distance, classified the Moringa Adans. populations into five major groups. The PCOA data confirmed the results of clustering. The results of this study revealed that R-ISSR markers could be efficiently used for genetic differentiation of the Moringa individuals. The primers used in this article are useful to detection of a high level of polymorphism and it can be used to guide future breeding studies and management of Moringa genus.","PeriodicalId":41224,"journal":{"name":"Journal of Medicinal Plants and By-products-JMPB","volume":"8 1","pages":"53-65"},"PeriodicalIF":0.6,"publicationDate":"2019-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46942804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}